Code | CSB-RP003674h |
Abbreviation | Recombinant Human GPI protein, partial |
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Size | $224 |
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The recombinant Human GPI protein synthesis in e.coli cells necessitates the incorporation of a DNA fragment encoding the Human GPI protein (2-554aa) into a plasmid vector, followed by the transformation of this vector into e.coli cells. After screening for positive cells, they are cultured and induced to express the GPI protein. The protein carries a N-terminal 6xHis tag. Cell lysis is performed to gather the recombinant Human GPI protein, which undergoes affinity purification and is then analyzed using SDS-PAGE and subsequent staining of the gel with Coomassie Brilliant Blue. The purity of the resulting recombinant Human GPI protein reaches up to 90%.
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