Recombinant Human Glutamate [NMDA] receptor subunit epsilon-3 (GRIN2C), partial

Code CSB-YP621798HU
MSDS
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Source Yeast
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Code CSB-EP621798HU
MSDS
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Source E.coli
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Code CSB-EP621798HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP621798HU
MSDS
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Source Baculovirus
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Code CSB-MP621798HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
GRIN2C
Uniprot No.
Alternative Names
GluN2C; Glutamate [NMDA] receptor subunit epsilon-3; Glutamate Receptor Ionotropic N Methyl D Aspartate 2C; Glutamate receptor ionotropic, NMDA 2C; Glutamate receptor, ionotropic, N methyl D aspartate 2C; Grin2c; N Methly D Aspartate Receptor Channel Subunit Epsilon 3; N Methyl D Aspartate Receptor Subtype 2C; N methyl D aspartate receptor subunit 2C; N-methyl D-aspartate receptor subtype 2C; NMDAR2C; NMDE3_HUMAN; NR2C; OTTHUMP00000041930
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Component of NMDA receptor complexes that function as heterotetrameric, ligand-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Channel activation requires binding of the neurotransmitter glutamate to the epsilon subunit, glycine binding to the zeta subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+). Sensitivity to glutamate and channel kinetics depend on the subunit composition (Probable). Plays a role in regulating the balance between excitatory and inhibitory activity of pyramidal neurons in the prefrontal cortex. Contributes to the slow phase of excitatory postsynaptic current, long-term synaptic potentiation, and learning.
Gene References into Functions
  1. ultra-rare variants with loss of function (frameshift, nonsense or splice site) in NMDARs genes like GRIN2C and GRIN2D may contribute to possible risk of schizophrenia PMID: 29317596
  2. Findings indicate that SNPs in the GRIN2C gene is associated with altered cue-induced brain activation that is related to craving for alcohol and relapse risk. PMID: 26289945
  3. these findings highlight the isoform-specific structural and functional differences within the 14-3-3 family of proteins, which determine GluN2C binding and its essential role in targeting the receptor to the cell surface PMID: 26229101
  4. The major depression subjects exhibited significantly higher expression levels of the NMDA receptor subunit genes GRIN2C. PMID: 24925192
  5. TNFalpha significantly upregulates NMDA-R2C mRNA expression, in differentiated, confluent, normal keratinocytes but not in involved or uninvolved psoriatic keratinocyte monolayers PMID: 24102971
  6. The authors identified and cloned sequences of NMDA receptor subunits 2A, 2B, and 2C from rat. They characterized the properties of the receptors composed of heteromeric 1-2A and heteromeric 1-2C and found that the latter had reduced sensitivity to magnesium and smaller conductance compared to the former. PMID: 1350383
  7. Functional compensation could occur to counteract the loss of one allele in GRIN2C and GRIN3 family genes. PMID: 22833210

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Subcellular Location
Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein.
Protein Families
Glutamate-gated ion channel (TC 1.A.10.1) family, NR2C/GRIN2C subfamily
Tissue Specificity
Mainly expressed in brain with predominant expression is in the cerebellum, also present in the hippocampus, amygdala, caudate nucleus, corpus callosum, subthalamic nuclei and thalamus. Detected in the heart, skeletal muscle and pancreas.
Database Links

HGNC: 4587

OMIM: 138254

KEGG: hsa:2905

STRING: 9606.ENSP00000293190

UniGene: Hs.436980

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