Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

GRIA3

Uniprot No.

P42263

Research Area

Neuroscience

Alternative Names

AMPA 3; AMPA selective glutamate receptor 3; AMPA-selective glutamate receptor 3; dJ1171F9.1; GluA3; GLUK3; GluR 3; GLUR C; GLUR K3; GluR-3; GluR-C; GluR-K3; GLUR3; GLURC; Glutamate ionotropic receptor AMPA type subunit 3; Glutamate receptor 3; Glutamate receptor C; Glutamate receptor ionotrophic AMPA 3; Glutamate receptor ionotropic; Glutamate receptor subunit 3; Glutamate receptor; ionotropic; AMPA 3; GRIA 3; Gria3; GRIA3_HUMAN; Ionotrophic Glutamate Receptor; MRX94

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

151-250aa

Target Protein Sequence

SLLGHYKWEKFVYLYDTERGFSILQAIMEAAVQNNWQVTARSVGNIKDVQEFRRIIEEMDRRQEKRYLIDCEVERINTILEQVVILGKHSRGYHYMLANL
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

28.0kDa

Protein Length

Partial

Tag Info

N-terminal 6xHis-SUMO-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The gene responsible for the Human GRIA3 protein (151-250aa) is incorporated into a plasmid vector, forming recombinant plasmid. The resulting recombinant plasmid is introduced into e.coli cells, from which cells survive in the presence of a specific antibiotic are selected. The selected e.coli cells containing the recombinant plasmid are cultured under conditions that facilitate the expression of the gene of interest. A N-terminal 6xHis-SUMO tag is linked to the protein. After expression, affinity purification is used to isolate and purify the recombinant Human GRIA3 protein from the cell lysate. Denaturing SDS-PAGE is employed to resolve the resulting recombinant Human GRIA3 protein, revealing a purity greater than 90%.

Target Background

Function

Receptor for glutamate that functions as ligand-gated ion channel in the central nervous system and plays an important role in excitatory synaptic transmission. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate.

Gene References into Functions

Results suggest a role for GluA3 channel activity in the regulation of sleep behavior in both mice and humans. PMID: 29016847
miR-330-3p up-regulated the total DNA methylation in non-small cell lung cancer cells, and co-IP-demonstrated GRIA3 was directly related with DNMT1 and DNMT3A. GRIA3 is a direct target of miR-330-3p. PMID: 28629431
This study showed the lower GluA3 mRNA levels in pregnant women. PMID: 28284346
the impaired surface expression of homomeric GluA3 receptors is caused by nonproductive assembly and aggregation to which LBD residues Tyr-454 and Arg-461 strongly contribute. PMID: 26912664
This study demonstrated that the GRIA3 protein was altered in auditory cortex patient with schizophreia. PMID: 25433904
An association was observed in migraine patients with the GRIA3 single nucleotide polymorphism rs3761555. PMID: 23772601
The ionotrophic glutamate receptors AMPA3 and AMPA3 were decreased in hippocampus in patient with multiple sclerosis. PMID: 23595422
The rs557762 and the TT haplotype in the 11th haplotype block of the GRIA3 gene were associated with feelings of guilt in females. PMID: 22429480
the promoter methylation of the GMR2 and GMR5 genes greatly decreased the risk of schizophrenia, and the expression level of the GRM2, GRM5, and GRIA3 genes increased significantly in patients in comparison to healthy controls. PMID: 23149219
SNP rs687577 associated with sleep duration and depression risk in Finnish women PMID: 21966062
GRIA3 plays a role as a mediator of tumor progression in pancreatic cancer downstream CUX1. PMID: 20689760
Two variants in the regulative regions of GRIA1 (rs2195450) and GRIA3 (rs3761555) genes resulted strongly associated with MA (P = 0.00002 and P = 0.0001, respectively), but not associated with MO PMID: 20579352
AQP3 gene isn't the responsible gene for this pedigree with auditory neuropathy. PMID: 20564826
This study demonistrated that the level of metabotropic glutamate receptor 2/3 is elevated in the prefrontal cortex in patient of major depression disorder. PMID: 19945495
The mGlu3 receptor is located on a number of GABAergic interneurons throughout the brain. mGlu3 receptor is one of two primary mGlu receptors that modulate the function of glia. The mGlu3 receptor is present as a postsynaptic receptor as well. PMID: 19933774
T lymphocytes from normal individuals, an alloprimed human T cell clone, and human T leukemia (Jurkat) cells all express high levels of GluR3, identical in sequence with brain GluR3. PMID: 12682273
cleavage and release to the extracellular milieu of the GluR3B peptide may in principle increase its antigenicity PMID: 17202328
study provides the genetic and functional evidence that mutant iGluR3 with altered kinetic properties is associated with moderate cognitive impairment in humans PMID: 17989220
results support the involvement of genes GRIA3 and GRIK2 in antidepressant treatment-emergent suicidal ideation PMID: 18593792
Aberrant GRIA3 transcripts with multi-exon duplications in a family with X-linked mental retardation are reported. PMID: 19449417

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Involvement in disease

Mental retardation, X-linked 94 (MRX94)

Subcellular Location

Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein.

Protein Families

Glutamate-gated ion channel (TC 1.A.10.1) family, GRIA3 subfamily

Database Links

HGNC: 4573

OMIM: 300699

KEGG: hsa:2892

STRING: 9606.ENSP00000264357

UniGene: Hs.377070

Code	CSB-EP009900HU
Abbreviation	Recombinant Human GRIA3 protein, partial
MSDS	MSDS Datasheet
Size	$224
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Glutamate receptor 3 (GRIA3), partial

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