Myostatin, also known as growth differentiation factor 8 (GDF8), is a protein that plays a key role in regulating muscle growth. It belongs to the TGF-β superfamily and is mainly produced in skeletal muscle cells. By inhibiting muscle cell growth and differentiation, myostatin helps maintain proper muscle mass.
This recombinant human MSTN protein is expressed in Mammalian cell and covers the 24-375aa amino acid region of the full-length protein. It includes an N-terminal 10xHis tag for easy purification. It is over 95% pure, has low endotoxin levels (<1.0 EU/μg), and shows verified biological activity.
The main potential applications of this recombinant protein include:
Note: The following applications are based on the known biological functions of this protein and scientific literature predictions. Our company has not validated all listed applications, and specific effects need to be verified by customers according to their experimental requirements. We recommend conducting small-scale preliminary experiments before formal studies.
1. Serving as a tool protein for in vitro functional studies to investigate the molecular mechanisms by which MSTN regulates skeletal muscle cell proliferation, differentiation, and muscle development—for example, through modulation of the SMAD2/3 signaling pathway and TET1 expression, which influences satellite cell differentiation and epigenetic modifications [1] [2];
2. Being used in muscle atrophy models related to chronic kidney disease, heart failure, and other conditions to study the role of MSTN in muscle wasting, protein degradation, and disease progression, as well as its potential as a biomarker or therapeutic target [3] [4];
3. Supporting the screening and validation of MSTN inhibitors or pathway-specific modulators to advance novel therapeutic strategies for muscle atrophy and metabolic disorders [3] [4];
4. Acting as a functional verification protein in animal breeding and gene editing research, helping to evaluate the impact of MSTN mutations on muscle growth, fat deposition, and production traits in livestock, thereby contributing to the molecular breeding of high-yield meat-producing animals [5].
In addition, its high purity and low endotoxin levels make this protein well-suited for various in vitro applications, such as cell culture, ELISA-based assays, and signaling pathway activation/inhibition studies.
References
1. Gao, L., Yang, M., Wei, Z., Gu, M., Yang, L., Bai, C., Wu, Y., & Li, G. MSTN Mutant Promotes Myogenic Differentiation by Increasing Demethylase TET1 Expression via the SMAD2/SMAD3 Pathway. International Journal of Biological Sciences. 2020; 16. https://doi.org/10.7150/ijbs.40551
2. Han, S., Gao, K., Chang, S., Choe, H., Paek, H., Quan, B., Liu, X., Yang, L., Lv, S., Yin, X., Quan, L., & Kang, J. miR-455-3p Is Negatively Regulated by Myostatin in Skeletal Muscle and Promotes Myoblast Differentiation.. Journal of agricultural and food chemistry. 2022 https://doi.org/10.1021/acs.jafc.2c02474
3. Bataille, S., Chauveau, P., Fouque, D., Aparicio, M., & Koppe, L. Myostatin and muscle atrophy during chronic kidney disease.. Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association. 2020 https://doi.org/10.1093/ndt/gfaa129
4. Paddock, S., & O'Meara, C. Steps towards therapeutically targeting the Activin Type II receptor for treating heart failure.. American journal of physiology. Heart and circulatory physiology. 2020 https://doi.org/10.1152/ajpheart.00004.2020
5. Finchum, R., Dilger, A., & Beever, J. PSIII-8 CRISPR editing of IGF2 and MSTN to enhance productivity. Journal of Animal Science. 2024 https://doi.org/10.1093/jas/skae234.577
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