Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

IL26

Uniprot No.

Q9NPH9

Research Area

Immunology

Alternative Names

AK155; IL 26; IL-26; IL26; IL26_HUMAN; Interleukin 26; Interleukin-26; Protein AK155

Species

Homo sapiens (Human)

Source

Yeast

Expression Region

22-171aa

Target Protein Sequence

KHKQSSFTKSCYPRGTLSQAVDALYIKAAWLKATIPEDRIKNIRLLKKKTKKQFMKNCQFQEQLLSFFMEDVFGQLQLQGCKKIRFVEDFHSLRQKLSHCISCASSAREMKSITRMKRIFYRIGNKGIYKAISELDILLSWIKKLLESSQ
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.

Mol. Weight

19.6kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Customer Reviews and Q&A

■ Customer Reviews

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■ Q&A

I would like to use this recombinant protein for an in vitro assay. I don't want E.coli expressed protein because I cannot afford to have any endotoxin present in my experiment. I'm trying to activate cells that are sensitive to IL6. I would like to try samples from the three other expression systems (not E.coli) to see which one works best. Have received any feedback regarding the activity if this protein.
Please conform the information above and send any other information that you think might be helpful to me.

Thanks for your inquiry.
Since you don't want E.coli expressed protein because you cannot afford to have any endotoxin present in your experiment. Therefore, it is recommended to order the protein expressed by other eukaryotic expression systems. The eukaryotic expression system itself is free of endotoxin, so the content will be extremely low but we can't guarantee 100% endotoxin free. We can provide endotoxin removal service free of charge. The final quality control standard for endotoxin level is less than 1.0 EU/ug.
Regarding the activity, I am sorry that we have not detected or received feedback from other customers, and we can't guarantee to be 100% active.
In theory, what we provide is mature full length and we use the method of affinity chromatography to purify under a very mild environment, it should be active.
The Yeast expressed protein has been ordered many times by other customers before,you can order YP protein in a small size to have a try first.

Target Background

Function

May play a role in local mechanisms of mucosal immunity and seems to have a proinflammatory function. May play a role in inflammatory bowel disease. Activates STAT1 and STAT3, MAPK1/3 (ERK1/2), JUN and AKT. Induces expression of SOCS3, TNF-alpha and IL-8, secretion of IL-8 and IL-10 and surface expression of ICAM1. Decreases proliferation of intestinal epithelial cells. Is inhibited by heparin.

Gene References into Functions

this paper shows that IL-26 is overexpressed in Behcet's disease and enhances Th17 related -cytokines PMID: 28811236
The varIL26 genotype is associated with reduced PMN capacity to kill bacteria. A varIL26 genotype is associated with decreased levels of anti-TNF-alpha in CD patients. IL26 may help explain the role of bactDNA as a risk factor of flare in CD patients. PMID: 28879509
IL-26 activates STAT1/3 and leads to the induction of IL-6 and IL-8 expression in non-transformed cells derived from human colon. PMID: 28852311
IL-26 levels are higher in synovial fluid compared to plasma in spondyloarthritis. IL-26 was identified in axial facet joints of spondyloarthritis patients. Myofibroblasts from the spondyloarthritis synovium produce large amounts of IL-26. IL-26 induces bone mineralization in human osteoblasts. PMID: 28365787
this study shows that IL-26 is a unique cationic protein more similar to a soluble pattern recognition receptor than to conventional cytokines PMID: 28356384
this study shows that single nucleotide polymorphism near IL-26 gene is associated with familial vitiligo and existence of halo nevi in Estonian patients PMID: 26429320
Our study indicates that IL-26 is a potential biomarker of disease severity in pediatric asthma without signs of Th2-mediated inflammation. PMID: 27029915
IL-26 is emerging as a potentially important player in host defense and may also be a pathogenic factor in the chronic inflammatory disorders of humans. [Review] PMID: 26202572
The negative influence of IL-26 on the anti-mycobacterial activity and its constitutive presence in both serum and monocyte supernatants prompt a proposal that IL26 as a candidate gene for tuberculosis susceptibility. PMID: 25157980
IL-26 differentially modulates the infection by different enveloped viruses. PMID: 23875025
Elevated levels of IL-26 in human gastric cancer promote proliferation and survival by modulating STAT1/STAT3 signaling. PMID: 23704922
IL-26 appears as a novel proinflammatory cytokine, located upstream of the proinflammatory cascade, that may constitute a promising target to treat rheumatoid arthritis and chronic inflammatory disorders. PMID: 23055831
Identify a second enhancer element positioned between IL26 and IFNG required for both IL26 and IFNG expression. One function of this enhancer is to facilitate recruitment of RNA polymerase II to promoters of both genes. PMID: 22622197
common polymorphisms in the IFNgamma/IL-26 gene region may contribute to sex bias in susceptibility to rheumatoid arthritis, by distorting the propensity of female carriers versus male carriers to contract this disease. PMID: 14558082
The active receptor complex for IL-26 is a heterodimer composed of two receptor proteins: IL-20 receptor 1 and IL-10 receptor 2. Signaling through the IL-26 receptor results in activation of STAT1 and STAT3. PMID: 14764663
sensitivity to recombinant interleukin-26(IL-26) of various cell lines strictly correlated with the expression of IL-20 receptor 1 and blocking antibodies against either IL-10 receptor 2 or IL-20 receptor 1 inhibited IL-26-dependent signal transduction PMID: 15178681

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Subcellular Location

Secreted.

Protein Families

IL-10 family

Tissue Specificity

Expressed in HVS transformed T-cells but not other T-cell lines or primary stimulated T-cells. Expressed in colonic T-cells including Th17 inflammatory T-cells; the expression is significantly increased in serum of patients with Crohn's disease (at protei

Database Links

HGNC: 17119

OMIM: 605679

KEGG: hsa:55801

STRING: 9606.ENSP00000229134

UniGene: Hs.272350

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