Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

KYNU

Uniprot No.

Q16719

Research Area

Signal Transduction

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

1-307aa

Target Protein Sequence

MEPSSLELPADTVQRIAAELKCHPTDERVALHLDEEDKLRHFRECFYIPKIQDLPPVDLSLVNKDENAIYFLGNSLGLQPKMVKTYLEEELDKWAKIAAYGHEVGKRPWITGDESIVGLMKDIVGANEKEIALMNALTVNLHLLMLSFFKPTPKRYKILLEAKAFPSDHYAIESQLQLHGLNIEESMRMIKPREGEETLRIEDILEVIEKEGDSIAVILFSGVHFYTGQHFNIPAITKAGQAKGCYVGFDLAHAVGNVELYLHDWGVDFACWCSYKYLNAGAGGIAGAFIHEKHAHTIKPARSEFFN
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

61.6 kDa

Protein Length

Full Length of Isoform 2

Tag Info

N-terminal GST-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The expression region of this recombinant Human KYNU covers amino acids 1-307. This KYNU protein is expected to have a theoretical molecular weight of 61.6 kDa. This protein is generated in a e.coli-based system. Fusion of the N-terminal GST tag into the KYNU encoding gene fragment was conducted, allowing for easier detection and purification of the KYNU protein in subsequent stages.

human kynureninase (KYNU) is a crucial enzyme in the tryptophan catabolic pathway, catalyzing the conversion of kynurenine to anthranilic acid. KYNU plays a pivotal role in regulating the levels of neuroactive kynurenine metabolites, influencing neurotransmission and immune responses. In neurobiology, KYNU is implicated in neurodegenerative diseases and psychiatric disorders due to its involvement in kynurenine metabolism. In immunology research, KYNU modulates immune tolerance and inflammation. Furthermore, KYNU's role in cancer metabolism underscores its significance in oncology. Investigating KYNU provides insights into tryptophan metabolism, offering potential applications in neurology, immunology, and cancer research, with implications for therapeutic strategies targeting these pathways.

Target Background

Function

Catalyzes the cleavage of L-kynurenine (L-Kyn) and L-3-hydroxykynurenine (L-3OHKyn) into anthranilic acid (AA) and 3-hydroxyanthranilic acid (3-OHAA), respectively. Has a preference for the L-3-hydroxy form. Also has cysteine-conjugate-beta-lyase activity.

Gene References into Functions

Our results suggest that tryptophan metabolism might dichotomously modulate immune responses, with KYNU as a switch between immunosuppressive versus inflammatory outcomes. PMID: 26725996
A rare variant at the KYNU gene is associated with essential hypertension in the Han Chinese population. PMID: 22012986
The Lys412Glu polymorphism of the KYNU gene in a hypertensive candidate chromosomal region is associated with essential hypertension in Han Chinese. PMID: 16080802

Involvement in disease

Hydroxykynureninuria (HYXKY); Vertebral, cardiac, renal, and limb defects syndrome 2 (VCRL2)

Subcellular Location

Cytoplasm, cytosol.

Protein Families

Kynureninase family

Tissue Specificity

Expressed in all tissues tested (heart, brain placenta, lung, liver, skeletal muscle, kidney and pancreas). Highest levels found in placenta, liver and lung. Expressed in all brain regions.

Database Links

HGNC: 6469

OMIM: 236800

KEGG: hsa:8942

STRING: 9606.ENSP00000264170

UniGene: Hs.470126

Code	CSB-EP621970HU(F2)
Abbreviation	Recombinant Human KYNU protein
MSDS	MSDS Datasheet
Size	$306
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Kynureninase (KYNU)

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