Code | CSB-RP067974h |
Abbreviation | Recombinant Human LEP protein, partial |
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Size | $224 |
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The recombinant Human LEP protein synthesis in e.coli cells necessitates the incorporation of a DNA fragment encoding the Human LEP protein (29-164aa) into a plasmid vector, followed by the transformation of this vector into e.coli cells. After screening for positive cells, they are cultured and induced to express the LEP protein. The protein carries a N-terminal 6xHis tag. Cell lysis is performed to gather the recombinant Human LEP protein, which undergoes affinity purification and is then analyzed using SDS-PAGE and subsequent staining of the gel with Coomassie Brilliant Blue. The purity of the resulting recombinant Human LEP protein reaches up to 90%.
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