Human Leptin,LEP ELISA kit

Instructions
Code CSB-E04649h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name leptin
Alternative Names FLJ94114 ELISA Kit; LEP ELISA Kit; LEP_HUMAN ELISA Kit; LEPD ELISA Kit; Leptin (murine obesity homolog) ELISA Kit; Leptin (obesity homolog, mouse) ELISA Kit; Leptin ELISA Kit; Leptin Murine Obesity Homolog ELISA Kit; Leptin Precursor Obesity Factor ELISA Kit; OB ELISA Kit; Obese protein ELISA Kit; Obese, mouse, homolog of ELISA Kit; Obesity ELISA Kit; Obesity factor ELISA Kit; Obesity homolog mouse ELISA Kit; Obesity Murine Homolog Leptin ELISA Kit; OBS ELISA Kit; OTTHUMP00000212285 ELISA Kit
Abbreviation LEP
Uniprot No. P41159
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.060 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision

 

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Linearity

 

To assess the linearity of the assay, samples were spiked with high concentrations of human Leptin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

 

Typical Data

 

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

Q&A and Customer Reviews

 Q&A
Q:

Is the kit Human Leptin, LEP ELISA kit (with catalogue number CSB-E04649h), applicable in urine samples?
If yes, could you send us any reposts based on the sensitivity, detection range, sample volume?

A:
Thanks for your inquiry. We suggest you test the sample types mentioned in the manual. If you want to test urine samples, you can do a pretest first because the component of urine is very complicated.

Target Data

Function Key player in the regulation of energy balance and body weight control. Once released into the circulation, has central and peripheral effects by binding LEPR, found in many tissues, which results in the activation of several major signaling pathways
Gene References into Functions
  1. chemerin and leptin are elevated and omentin-1 and visfatin levels are decreased in Gestational diabetes mellitus women complicated by obesity. PMID: 29337272
  2. study demonstrated novel molecular events for leptin-induced inflammation in ligamentum flavum (LF) tissue by promoting IL-6 expression and thus might have potential implications for clarifying the mechanism underlying LF fibrosis and hypertrophy. PMID: 29436483
  3. Leptin protein was significantly elevated in pre-liver transplantation alcoholics and was lowered post-transplantation. PMID: 29912265
  4. The AA genotype of leptin rs7799039 is associated with metabolic syndrome and higher serum leptin levels in Egyptian women PMID: 27966294
  5. In critically ill heart failure patients, the circadian rhythm of plasma leptin concentration seems to be preserved during the first but not during the third day after admission. PMID: 29527917
  6. a potential link between gastric leptin and microbial-derived metabolites in the context of obesity and diabetes PMID: 29851973
  7. Results show that LEP rs7799039 allele A was slightly associated with reduced total and LDL cholesterol suggesting that LEP genetic variant may be useful biomarker of cardiometabolic risk in obese patients. PMID: 28975585
  8. The aim of this study was to determine serum ghrelin and leptin levels in obese and lean Saudi women with Polycystic ovary syndrome and to investigate their relationship to the metabolic profiles in these women. PMID: 30131073
  9. The serum leptin level does not differ significantly between non-obese OSA patients with moderate/severe and snoring/mild OSA. PMID: 29174427
  10. Leptin concentrations are higher in the obese group irrespective of their glucose tolerance PMID: 29108900
  11. Leptin-to-adiponectin ratio and IL-6 were elevated in men with prostate cancer. Leptin, chemerin and IL-6 were associated with Gleason score. The relationships between leptin, chemerin and IL-6 were dependent on each other. PMID: 29465157
  12. Results provide new evidences for a modulatory effect of leptin in regulation of ovarian cancer cell invasion by stimulating MMP7 expression via ERK and JNK pathways. PMID: 28885729
  13. this study shows that leptin regulates the pro-inflammatory response in human epidermal keratinocytes PMID: 29468452
  14. AMH, IGF1 and leptin levels in follicular fluid have no relation to the fertility disorders caused by endometriosis or fallopian tube damage, though they are biomarkers for anovulatory fertility disorders. PMID: 29595066
  15. Our data provide evidence that leptin-mediated OPN upregulation promote TH2 inflammation in AR and this process is achieved through the alpha4 integrin and PI3K/AKT signaling pathways. PMID: 29885866
  16. By functional analysis based on a set of 1129 proteins from 494 obese subjects study identified and validated FAM46A as a trans regulator for leptin. PMID: 29234017
  17. Cord blood klotho levels were inversely correlated with leptin and insulin levels at birth PMID: 29648999
  18. Estradiol and physical activity were stronger predictors of leptin at menses, while sexual activity was a stronger predictor of leptin at ovulation. These findings suggest that predictors of serum leptin, and possibly energy storage and expenditure, vary across the menstrual cycle. PMID: 29454804
  19. leptin, leptin receptor and apelin receptor genes are associated with susceptibility to coronary artery disease and hypertension PMID: 29883719
  20. Serum levels of leptin (and adiponectin) were significantly and negatively associated with bone mineral density in patients with knee osteoarthritis. PMID: 29343264
  21. This study provides evidence that polymorphisms in the LEP and LEPR genes are associated with the magnitude of the effects of regular physical activity on glucose and LDL-C levels, respectively. In addition, we found the association of the G allele of the LEPR polymorphism with body mass and BMI PMID: 29373433
  22. Increased levels of mucosal leptin may interact with mast cells and the nervous system to contribute to the pathogenesis of diarrhea-predominant irritable bowel syndrome. PMID: 29358881
  23. Data suggest that expression of aquaporin-9 in term placenta is up-regulated by leptin. These studies used placental explants following term birth via cesarean section. PMID: 28942694
  24. by analyzing different estrogen receptor-alpha(ER-a)-positive and ER-a-negative breast cancer cell lines, we defined the role of CCN5 in the leptin-mediated regulation of growth and invasive capacity. PMID: 29370782
  25. LEP-rs7799039 was significantly associated with an increased risk for excessive gestational weight gain. PMID: 29241578
  26. Higher serum leptin levels can be used as a risk factor for childhood obesity. PMID: 29412799
  27. H. pylori infection decreased serum concentrations of leptin and obestatin in Mexican schoolchildren, suggesting alterations in regulating appetite and energy homeostasis. PMID: 28422951
  28. Data suggest that the stimulatory effects of leptin on proliferative activity of human normal prostate and prostate cancer cell lines require the expression of leptin receptor variant 1 in the affected cells. PMID: 29115533
  29. The findings suggest that the LEP G2548A genetic polymorphism may increase the susceptibility of cancers in non-Hodgkin's lymphoma (meta-analysis). PMID: 25536622
  30. Features of poor prognosis such as tumor invasion, lymph node involvement and distant metastasis have been recorded in several cancer types with higher levels of leptin and/or Ob-R. This review will describe the current scenario in a precise manner. In general, obesity indicates poor prognosis in cancer patients. [Review] PMID: 29158066
  31. Clinical data on secondary hyperparathyroidism, mainly derived from patients with chronic kidney disease, indicate a potential inverse association between leptin (LEP) and parathyroid hormone (PTH) in some, but not all studies. [REVIEW] PMID: 28730419
  32. Data suggest that transient increases in plasma adiponectin levels and transient decreases in plasma leptin levels occur following high-intensity interval exercise in overweight and obese young women. These studies were conducted in Turkey in formerly sedentary women ages 20-30 years. PMID: 28857629
  33. There was no simple, linear association of leptin and physical activity in adults with anorexia nervosa. PMID: 29099750
  34. Neoplastic cells appeared unable to downregulate NOX5 mRNA expression under leptin. Leptin emerged as a potential activator of ROS production in human epithelial mammary cells, where the ROS production was apparently linked to NOX5 activation. This novel finding could shed light on the potential role of obesity-associated hyperleptinemia in mammary cells via the activation of NOX enzymes PMID: 29048637
  35. Data suggest that serum leptin may be a potential biomarker for predicting sarcopenic obesity and physical performance in knee osteoarthritis. PMID: 28374624
  36. Leptin synergizes with IL-1beta in inducing ELF3 expression in chondrocytes. PMID: 29550824
  37. The nitrogen stable isotopic ratio in hair is positively associated with serum leptin levels. PMID: 29081462
  38. Heterozygous genotype AG of Single Nucleotide Polymorphism -2548 G/A of leptin gene (LEP) may reduce the risk of endometrial cancer independently of overall adiposity of an individual. Allele A alone of the studied polymorphism may be a risk factor of this malignancy in obese women, whereas allele G may decrease the hazard in these patients. PMID: 28964972
  39. Pregnant adolescents with leptin concentration over 20 ng/mL showed a greater gestational weight gain. Leptin concentration correlated with length and weight of the newborn. PMID: 28953229
  40. LEP and LEPR single-nucleotide polymorphisms are not associated with genetic susceptibility to systemic lupus erythematosus. PMID: 28244652
  41. The upregulation of miR27 inhibits the pathogenesis of osteoarthritis by targeting leptin and inhibiting the NF-kappaB signaling pathway. PMID: 28627582
  42. There was a direct association between the polymorphisms in LEP rs7799039 and ADIPOQ rs1501299 with overweight/obesity, and these genotypes affected the response to breast cancer chemotherapeutic treatment in Mexican women PMID: 28416193
  43. Habitual intake of SFA, MUFA, and n-3 fatty acids were associated with leptin gene expression in visceral and subcutaneous adipose tissues, suggesting an important role of quality and quantity of fatty acids intake in adipose tissue to regulate leptin expression. PMID: 29031395
  44. The studies established a potential link between leptin and adipocyte insulin responsiveness in an NOS2 dependent manner. PMID: 28739528
  45. Leptin serum levels drop following bariatric surgery and is predictive of disease remission in type 2 diabetes mellitus patients. PMID: 28734574
  46. alterations in leptin and SCD-1 in HCU patients PMID: 28801090
  47. leptin may have a role in androgenetic alopecia in men PMID: 28423238
  48. study is the first to describe the mechanism of leptin-promoted lymphangiogenesis by upregulating VEGF-C expression in chondrosarcomas. PMID: 27345723
  49. The study suggested that leptin accelerated the epithelial mesenchymal transition of A549cells through inhibiting autophagy via PI3K/Akt/mTOR pathway. PMID: 29524411
  50. Studied adiponectin (ADIPOQ), leptin (LEP), leptin receptor (LEPR), and resistin (RETN) single nucleotide polymorphisms and their association in obesity. Results showed that ADIPOQ 4522CPMID: 28195351
  51. the association between adipokine levels and bone mineral density varies according to the menopause and central obesity status. PMID: 27873074
  52. Mendelian randomization study estimating the causal effect of leptin on Alzheimer disease (AD) did not find an effect of genetically predicted circulating leptin and sOB-R levels on AD. As such, this study suggests that leptin is unlikely to be a major contributor to AD, although the wide confidence intervals preclude a definitive assessment. PMID: 28419997
  53. Leptin levels were not altered in dementia. PMID: 28421328
  54. Leptin, and leptin receptor polymorphisms and breast cancer risk in a Chinese cohort of women. PMID: 29336592
  55. Obesity was associated with higher expression of NILCO molecules (Notch-IL1-leptin) in type II endometrial cancer. PMID: 28659656
  56. SLL might be beneficial as a useful biomarker in preclinical setting of NSCLC to guide detecting the lung cancer subtypes as well as monitoring the patients PMID: 28643734
  57. These data confirm that variations in the Lep gene as well as in the Lepr gene could play a role in weight gain. PMID: 28628399
  58. The discovery of the obese gene and the demonstration that its encoded protein leptin can reverse obesity due to genetic deficiency of the hormone were landmark discoveries in endocrinology and metabolism. Regarding leptin's role in physiology, it is now established that falling leptin levels are a key signal of the starved state in mice and humans. PMID: 28648981
  59. Patients with AS had higher serum TNF-alpha and leptin concentrations than controls PMID: 28534699
  60. Low plasma leptin level at intensive care unit entry was associated with the occurrence of delirium in critically ill patients. PMID: 28495595
  61. In female patients with panic disorder, lower leptin serum levels were associated with greater severity of psychopathological manifestations. PMID: 28551488
  62. Serum leptin may be a biomarker for adiposity in infancy in preterm very-low-birth-weight infants and could predict subsequent risk for abnormalities of metabolism, obesity, and cardiovascular risk. PMID: 28099428
  63. Data suggest that, in type 1 diabetes, elevated leptin is related to decreased cardiopulmonary fitness/exercise tolerance in adolescents/young adults; this relationship is independent of insulin resistance and adiposity/body mass index. This study was conducted in Colorado. PMID: 28336216
  64. Leptin treatment increased cell cycle progression and proliferation, and the expression of Notch receptors, ligands and targeted molecules in pancreatic tumor cells. PMID: 27999190
  65. placental leptin and LEPR protein expression may influence placental growth and thus, birth weight. PMID: 28351089
  66. Depression was associated with a decrease and increase in leptin respectively in normal-overweight and obese men and women diabetes patients. PMID: 28197877
  67. The genotype-risk associations were examined between LEP (rs2167270) and obesity-related phenotypes and other comorbidities in Sucre, Venezuela. The relation to BMI for the G/G phenotype was 25.04 +/- 4.20 kg/m2, for A/A/ it was 25.87 +/- 4.08 kg/m2, and for G/A it was 27.02 +/- 4.08 kg/m2. PMID: 27622441
  68. these findings indicate that leptin may promote tumor growth and metastasis by stimulating IL-8 production in tumor-associated macrophage PMID: 27588409
  69. Serum leptin levels were decreased following individualized resistance exercise in lean women with fibromyalgia. PMID: 28288611
  70. Review/Meta-analysis: genetic polymorphisms in leptin, adiponectin and their receptors affect the development and progression of prostate cancer. PMID: 27768592
  71. In prevalent kidney transplant recipients, lower serum leptin was an independent predictor of death. PMID: 27666945
  72. Taken together, these results suggest that leptin, in concert with other stimuli in the micro milieu, may contribute to the development of psoriasis. PMID: 27488462
  73. These findings are consistent with higher levels of serum leptin being associated with asthma regardless of age, and low adiponectin levels being associated with asthma in adults only. PMID: 27473714
  74. These findings suggest that specific MFN2 mutations cause tissue-selective mitochondrial dysfunction with increased adipocyte proliferation and survival and confirm a novel form of excess adiposity with paradoxical suppression of leptin expression. PMID: 28414270
  75. The G-allele of leptin gene polymorphism (G2548A) was associated with increased BP levels during pregnancy and the postpartum. PMID: 28077420
  76. Study demonstrates that fasting induces changes in DNA methylation. This was shown in LEP and ADIPOQ promoters in subcutaneous adipose tissue among normal birth weight (NBW) but not low birth weight (LBW) subjects. The altered epigenetic flexibility in LBW subjects might contribute to their differential response to fasting, adipokine levels, and increased risk of metabolic disease. PMID: 28439315
  77. We conclude that elevated plasma leptin levels are associated with impaired left ventricular diastolic function in patients with coronary artery disease independently of obesity and other confounding variables. PMID: 27524739
  78. The results of our study suggest an association between adiponectin gene rs266729 as well as leptin gene rs2167270 polymorphisms and Gestational diabetes mellitus PMID: 28050671
  79. This study shows that leptin is measurable in exhaled breath condensate (EBC) in children and that exhaled breath condensate-leptin levels are significantly higher in the obese subjects and in asthmatic ones compared with healthy subjects. PMID: 28952460
  80. Leptin is the most important adipokine in anorexia nervosa (AN). Leptin and free leptin index are the only factors that are influenced not only by the fat content. PMID: 28604943
  81. Women spent almost 60% of their time sitting throughout pregnancy. In cross-sectional analyses, an association of sedentary time at 24weeks was found with increased total cholesterol and HDL. More sedentary time was associated with lower IL-6 at 24weeks and with higher IL-10, TNF-alpha and leptin levels at 32weeks of pregnancy. PMID: 27591509
  82. When stratified by menopausal status free leptin index and soluble leptin receptor remained as risk factors irrespective of menopausal status while LEPR gene K109R A/G polymorphism remained as a risk factor only in the postmenopausal group. PMID: 28190851
  83. Infants carrying the GG genotype of the LEP rs7799039 polymorphism were 2.12 times more likely to be born large for gestational age (LGA) than those carrying the GA + AA genotypes. Newborns carrying the TG or GG genotype of the ADIPOQ rs2241766 polymorphism were 1.88 times more likely to be born LGA than those carrying the TT genotype. No association was found between the FTO gene polymorphism and newborn weight status. PMID: 27392994
  84. Analysis of human aortic valve stenosis leaflets revealed de novo leptin synthesis. PMID: 27143353
  85. serum levels of leptin were positively associated with RA activity level in patients with RA, suggesting that this adipokine may act as pro-inflammatory cytokine in this disease. PMID: 27690205
  86. Results demonstrated that leptin promoted breast cancer cells epithelial-mesenchymal transition, visibly activated the PI3K/AKT signaling pathway, and upregulated PKM2 expression. PMID: 27769315
  87. Elevated level of soluble tumor necrosis factor receptors 1 and lower level of leptin are associated with higher developmental outcomes in infants between the ages of 6 and 24 months. PMID: 28238825
  88. In the infertile me, serum leptin level was significantly and inversely correlated with BMI. PMID: 28164564
  89. we speculate that up-regulation of leptin and ARMS2 forms part of an important survival mechanism to compensate for placental growth discordance in monochorionic diamniotic twin pregnancies PMID: 28303777
  90. The results indicated that globular adiponectin inhibited the prooncogenic effects of leptin via AdipoR2-mediated suppression of UHRF1 PMID: 28285359
  91. Endoplasmic reticulum stress-induced CHOP activation in the brain is a mechanistic link in the palmitate-induced negative regulation of leptin and IGF1. PMID: 27555288
  92. OB-Rb, RhoA/ROCK, PI3K/AKT, JAK/STAT pathways and NF-kB activation are involved in leptin-induced upA expression. PMID: 28104444
  93. Our data suggest that genetic variability in the LEP gene might be associated with male infertility warranting further confirmation and mechanistic investigations. PMID: 27813378
  94. Higher leptin concentration was associated with increased risk of impaired physical function in older adults. PMID: 27515676
  95. disturbance of reciprocal relationships between adipsin and leptin in obesity is associated with the development of insulin resistance PMID: 28864895
  96. The leptin SNP rs7799039 (G2548A) was associated to high sensitivity-C-reactive protein levels at both ages. PMID: 28350535
  97. High leptin/adiponectin ratio in pregnancy, particularly in those with gestational diabetes, is associated with an unfavorable CVD risk profile during follow-up. PMID: 28068986
  98. leptin-adiponectin imbalance, as reflected by an increase in leptin/adiponectin ratio, was found to be a better diagnostic biomarker for MS than leptin or adiponectin alone. PMID: 29020116
  99. Leptin knockdown suppressed MUC5AC production and secretion induced by IL-13 in human bronchial epithelial cells. PMID: 28942146
  100. Leptin levels increase significantly within 3 months of fetal pancreatic stem cell transplant in patients with type 1 diabetes mellitus. PMID: 28260466

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Involvement in disease Leptin deficiency (LEPD)
Subcellular Location Secreted
Protein Families Leptin family
Tissue Specificity Adipose tissue is the main source of leptin it is also produced by other peripheral tissues such as the skeletal muscle (PubMed:7789654, PubMed:16052473, PubMed:12448771). Expressed by intercalated and striated tracts of submandibular and parotid salivary
Database Links

HGNC: 6553

OMIM: 164160

KEGG: hsa:3952

STRING: 9606.ENSP00000312652

UniGene: Hs.194236

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