Human Leptin,LEP ELISA kit

Code CSB-E04649h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Human LEP ELISA Kit was designed for the quantitative measurement of Human LEP protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.060 ng/mL.

Target Name leptin
Alternative Names FLJ94114 ELISA Kit; LEP ELISA Kit; LEP_HUMAN ELISA Kit; LEPD ELISA Kit; Leptin (murine obesity homolog) ELISA Kit; Leptin (obesity homolog, mouse) ELISA Kit; Leptin ELISA Kit; Leptin Murine Obesity Homolog ELISA Kit; Leptin Precursor Obesity Factor ELISA Kit; OB ELISA Kit; Obese protein ELISA Kit; Obese, mouse, homolog of ELISA Kit; Obesity ELISA Kit; Obesity factor ELISA Kit; Obesity homolog mouse ELISA Kit; Obesity Murine Homolog Leptin ELISA Kit; OBS ELISA Kit; OTTHUMP00000212285 ELISA Kit
Abbreviation LEP
Uniprot No. P41159
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.060 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision

 

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Linearity

 

To assess the linearity of the assay, samples were spiked with high concentrations of human Leptin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

 

Typical Data

 

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

Materials provided
  • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human LEP antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
  • Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
  • One vial Biotin-labeled LEP antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
  • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
  • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody solution.
  • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
  • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
  • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
  • One vial TMB Substrate (10 ml/bottle) --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
  • One vial Stop Solution (10 ml/bottle) --- Stop the color reaction. The solution color immediately turns from blue to yellow.
  • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
  • An instruction manual
Materials not provided
  • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
  • An incubator can provide stable incubation conditions up to 37°C±5°C.
  • Centrifuge
  • Vortex
  • Squirt bottle, manifold dispenser, or automated microplate washer
  • Absorbent paper for blotting the microtiter plate
  • 50-300ul multi-channel micropipette
  • Pipette tips
  • Single-channel micropipette with different ranges
  • 100ml and 500ml graduated cylinders
  • Deionized or distilled water
  • Timer
  • Test tubes for dilution
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

Customer Reviews and Q&A

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 Q&A
Q:

Is the kit Human Leptin, LEP ELISA kit (with catalogue number CSB-E04649h), applicable in urine samples?
If yes, could you send us any reposts based on the sensitivity, detection range, sample volume?

A:
Thanks for your inquiry. We suggest you test the sample types mentioned in the manual. If you want to test urine samples, you can do a pretest first because the component of urine is very complicated.

Target Background

Function
(From Uniprot)
Key player in the regulation of energy balance and body weight control. Once released into the circulation, has central and peripheral effects by binding LEPR, found in many tissues, which results in the activation of several major signaling pathways. In the hypothalamus, acts as an appetite-regulating factor that induces a decrease in food intake and an increase in energy consumption by inducing anorexinogenic factors and suppressing orexigenic neuropeptides, also regulates bone mass and secretion of hypothalamo-pituitary-adrenal hormones. In the periphery, increases basal metabolism, influences reproductive function, regulates pancreatic beta-cell function and insulin secretion, is pro-angiogenic for endothelial cell and affects innate and adaptive immunity. In the arcuate nucleus of the hypothalamus, activates by depolarization POMC neurons inducing FOS and SOCS3 expression to release anorexigenic peptides and inhibits by hyperpolarization NPY neurons inducing SOCS3 with a consequent reduction on release of orexigenic peptides. In addition to its known satiety inducing effect, has a modulatory role in nutrient absorption. In the intestine, reduces glucose absorption by enterocytes by activating PKC and leading to a sequential activation of p38, PI3K and ERK signaling pathways which exerts an inhibitory effect on glucose absorption. Acts as a growth factor on certain tissues, through the activation of different signaling pathways increases expression of genes involved in cell cycle regulation such as CCND1, via JAK2-STAT3 pathway, or VEGFA, via MAPK1/3 and PI3K-AKT1 pathways. May also play an apoptotic role via JAK2-STAT3 pathway and up-regulation of BIRC5 expression. Pro-angiogenic, has mitogenic activity on vascular endothelial cells and plays a role in matrix remodeling by regulating the expression of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs). In innate immunity, modulates the activity and function of neutrophils by increasing chemotaxis and the secretion of oxygen radicals. Increases phagocytosis by macrophages and enhances secretion of pro-inflammatory mediators. Increases cytotoxic ability of NK cells. Plays a pro-inflammatory role, in synergy with IL1B, by inducing NOS2 wich promotes the production of IL6, IL8 and Prostaglandin E2, through a signaling pathway that involves JAK2, PI3K, MAP2K1/MEK1 and MAPK14/p38. In adaptive immunity, promotes the switch of memory T-cells towards T helper-1 cell immune responses. Increases CD4(+)CD25(-) T-cell proliferation and reduces autophagy during TCR (T-cell receptor) stimulation, through MTOR signaling pathway activation and BCL2 up-regulation.
Gene References into Functions
  1. chemerin and leptin are elevated and omentin-1 and visfatin levels are decreased in Gestational diabetes mellitus women complicated by obesity. PMID: 29337272
  2. study demonstrated novel molecular events for leptin-induced inflammation in ligamentum flavum (LF) tissue by promoting IL-6 expression and thus might have potential implications for clarifying the mechanism underlying LF fibrosis and hypertrophy. PMID: 29436483
  3. Leptin protein was significantly elevated in pre-liver transplantation alcoholics and was lowered post-transplantation. PMID: 29912265
  4. The AA genotype of leptin rs7799039 is associated with metabolic syndrome and higher serum leptin levels in Egyptian women PMID: 27966294
  5. In critically ill heart failure patients, the circadian rhythm of plasma leptin concentration seems to be preserved during the first but not during the third day after admission. PMID: 29527917
  6. a potential link between gastric leptin and microbial-derived metabolites in the context of obesity and diabetes PMID: 29851973
  7. Results show that LEP rs7799039 allele A was slightly associated with reduced total and LDL cholesterol suggesting that LEP genetic variant may be useful biomarker of cardiometabolic risk in obese patients. PMID: 28975585
  8. The aim of this study was to determine serum ghrelin and leptin levels in obese and lean Saudi women with Polycystic ovary syndrome and to investigate their relationship to the metabolic profiles in these women. PMID: 30131073
  9. The serum leptin level does not differ significantly between non-obese OSA patients with moderate/severe and snoring/mild OSA. PMID: 29174427
  10. Leptin concentrations are higher in the obese group irrespective of their glucose tolerance PMID: 29108900
  11. Leptin-to-adiponectin ratio and IL-6 were elevated in men with prostate cancer. Leptin, chemerin and IL-6 were associated with Gleason score. The relationships between leptin, chemerin and IL-6 were dependent on each other. PMID: 29465157
  12. Results provide new evidences for a modulatory effect of leptin in regulation of ovarian cancer cell invasion by stimulating MMP7 expression via ERK and JNK pathways. PMID: 28885729
  13. this study shows that leptin regulates the pro-inflammatory response in human epidermal keratinocytes PMID: 29468452
  14. AMH, IGF1 and leptin levels in follicular fluid have no relation to the fertility disorders caused by endometriosis or fallopian tube damage, though they are biomarkers for anovulatory fertility disorders. PMID: 29595066
  15. Our data provide evidence that leptin-mediated OPN upregulation promote TH2 inflammation in AR and this process is achieved through the alpha4 integrin and PI3K/AKT signaling pathways. PMID: 29885866
  16. By functional analysis based on a set of 1129 proteins from 494 obese subjects study identified and validated FAM46A as a trans regulator for leptin. PMID: 29234017
  17. Cord blood klotho levels were inversely correlated with leptin and insulin levels at birth PMID: 29648999
  18. Estradiol and physical activity were stronger predictors of leptin at menses, while sexual activity was a stronger predictor of leptin at ovulation. These findings suggest that predictors of serum leptin, and possibly energy storage and expenditure, vary across the menstrual cycle. PMID: 29454804
  19. leptin, leptin receptor and apelin receptor genes are associated with susceptibility to coronary artery disease and hypertension PMID: 29883719
  20. Serum levels of leptin (and adiponectin) were significantly and negatively associated with bone mineral density in patients with knee osteoarthritis. PMID: 29343264
  21. This study provides evidence that polymorphisms in the LEP and LEPR genes are associated with the magnitude of the effects of regular physical activity on glucose and LDL-C levels, respectively. In addition, we found the association of the G allele of the LEPR polymorphism with body mass and BMI PMID: 29373433
  22. Increased levels of mucosal leptin may interact with mast cells and the nervous system to contribute to the pathogenesis of diarrhea-predominant irritable bowel syndrome. PMID: 29358881
  23. Data suggest that expression of aquaporin-9 in term placenta is up-regulated by leptin. These studies used placental explants following term birth via cesarean section. PMID: 28942694
  24. by analyzing different estrogen receptor-alpha(ER-a)-positive and ER-a-negative breast cancer cell lines, we defined the role of CCN5 in the leptin-mediated regulation of growth and invasive capacity. PMID: 29370782
  25. LEP-rs7799039 was significantly associated with an increased risk for excessive gestational weight gain. PMID: 29241578
  26. Higher serum leptin levels can be used as a risk factor for childhood obesity. PMID: 29412799
  27. H. pylori infection decreased serum concentrations of leptin and obestatin in Mexican schoolchildren, suggesting alterations in regulating appetite and energy homeostasis. PMID: 28422951
  28. Data suggest that the stimulatory effects of leptin on proliferative activity of human normal prostate and prostate cancer cell lines require the expression of leptin receptor variant 1 in the affected cells. PMID: 29115533
  29. The findings suggest that the LEP G2548A genetic polymorphism may increase the susceptibility of cancers in non-Hodgkin's lymphoma (meta-analysis). PMID: 25536622
  30. Features of poor prognosis such as tumor invasion, lymph node involvement and distant metastasis have been recorded in several cancer types with higher levels of leptin and/or Ob-R. This review will describe the current scenario in a precise manner. In general, obesity indicates poor prognosis in cancer patients. [Review] PMID: 29158066
  31. Clinical data on secondary hyperparathyroidism, mainly derived from patients with chronic kidney disease, indicate a potential inverse association between leptin (LEP) and parathyroid hormone (PTH) in some, but not all studies. [REVIEW] PMID: 28730419
  32. Data suggest that transient increases in plasma adiponectin levels and transient decreases in plasma leptin levels occur following high-intensity interval exercise in overweight and obese young women. These studies were conducted in Turkey in formerly sedentary women ages 20-30 years. PMID: 28857629
  33. There was no simple, linear association of leptin and physical activity in adults with anorexia nervosa. PMID: 29099750
  34. Neoplastic cells appeared unable to downregulate NOX5 mRNA expression under leptin. Leptin emerged as a potential activator of ROS production in human epithelial mammary cells, where the ROS production was apparently linked to NOX5 activation. This novel finding could shed light on the potential role of obesity-associated hyperleptinemia in mammary cells via the activation of NOX enzymes PMID: 29048637
  35. Data suggest that serum leptin may be a potential biomarker for predicting sarcopenic obesity and physical performance in knee osteoarthritis. PMID: 28374624
  36. Leptin synergizes with IL-1beta in inducing ELF3 expression in chondrocytes. PMID: 29550824
  37. The nitrogen stable isotopic ratio in hair is positively associated with serum leptin levels. PMID: 29081462
  38. Heterozygous genotype AG of Single Nucleotide Polymorphism -2548 G/A of leptin gene (LEP) may reduce the risk of endometrial cancer independently of overall adiposity of an individual. Allele A alone of the studied polymorphism may be a risk factor of this malignancy in obese women, whereas allele G may decrease the hazard in these patients. PMID: 28964972
  39. Pregnant adolescents with leptin concentration over 20 ng/mL showed a greater gestational weight gain. Leptin concentration correlated with length and weight of the newborn. PMID: 28953229
  40. LEP and LEPR single-nucleotide polymorphisms are not associated with genetic susceptibility to systemic lupus erythematosus. PMID: 28244652
  41. The upregulation of miR27 inhibits the pathogenesis of osteoarthritis by targeting leptin and inhibiting the NF-kappaB signaling pathway. PMID: 28627582
  42. There was a direct association between the polymorphisms in LEP rs7799039 and ADIPOQ rs1501299 with overweight/obesity, and these genotypes affected the response to breast cancer chemotherapeutic treatment in Mexican women PMID: 28416193
  43. Habitual intake of SFA, MUFA, and n-3 fatty acids were associated with leptin gene expression in visceral and subcutaneous adipose tissues, suggesting an important role of quality and quantity of fatty acids intake in adipose tissue to regulate leptin expression. PMID: 29031395
  44. The studies established a potential link between leptin and adipocyte insulin responsiveness in an NOS2 dependent manner. PMID: 28739528
  45. Leptin serum levels drop following bariatric surgery and is predictive of disease remission in type 2 diabetes mellitus patients. PMID: 28734574
  46. alterations in leptin and SCD-1 in HCU patients PMID: 28801090
  47. leptin may have a role in androgenetic alopecia in men PMID: 28423238
  48. study is the first to describe the mechanism of leptin-promoted lymphangiogenesis by upregulating VEGF-C expression in chondrosarcomas. PMID: 27345723
  49. The study suggested that leptin accelerated the epithelial mesenchymal transition of A549cells through inhibiting autophagy via PI3K/Akt/mTOR pathway. PMID: 29524411
  50. Studied adiponectin (ADIPOQ), leptin (LEP), leptin receptor (LEPR), and resistin (RETN) single nucleotide polymorphisms and their association in obesity. Results showed that ADIPOQ 4522CPMID: 28195351

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Involvement in disease Leptin deficiency (LEPD)
Subcellular Location Secreted.
Protein Families Leptin family
Tissue Specificity Adipose tissue is the main source of leptin. It is also produced by other peripheral tissues such as the skeletal muscle. Expressed by intercalated and striated tracts of submandibular and parotid salivary gland intralobular ducts. Detected by fundic epit
Database Links

HGNC: 6553

OMIM: 164160

KEGG: hsa:3952

STRING: 9606.ENSP00000312652

UniGene: Hs.194236

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