Recombinant Human Transforming growth factor beta activator LRRC32 (LRRC32), partial

Code CSB-YP618886HU
MSDS
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Source Yeast
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Code CSB-EP618886HU
MSDS
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Source E.coli
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Code CSB-EP618886HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP618886HU
MSDS
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Source Baculovirus
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Code CSB-MP618886HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
LRRC32
Uniprot No.
Alternative Names
D11S833E; GARP; Garpin; Glycoprotein A repetitions predominant; Leucine rich repeat containing 32; Leucine-rich repeat-containing protein 32; LRC32_HUMAN; LRRC32
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Key regulator of transforming growth factor beta (TGFB1, TGFB2 and TGFB3) that controls TGF-beta activation by maintaining it in a latent state during storage in extracellular space. Associates specifically via disulfide bonds with the Latency-associated peptide (LAP), which is the regulatory chain of TGF-beta, and regulates integrin-dependent activation of TGF-beta. Able to outcompete LTBP1 for binding to LAP regulatory chain of TGF-beta. Controls activation of TGF-beta-1 (TGFB1) on the surface of activated regulatory T-cells (Tregs). Required for epithelial fusion during palate development by regulating activation of TGF-beta-3 (TGFB3).
Gene References into Functions
  1. GARP is a surface molecule of regulatory T cells with roles in the regulatory function and TGF-beta releasing [review] PMID: 27095576
  2. Data show that the Treg activation marker GARP (glycoprotein A repetitions predominant) is expressed on primary melanoma. PMID: 27248166
  3. High GARP expression is associated with pancreatic cancer and liver metastases from colorectal cancer. PMID: 26885615
  4. study showed that stimulated, human B lymphocytes produce active TGF-beta1 from surface GARP/latent TGF-beta1 complexes with isotype switching to IgA production. PMID: 28607112
  5. GARP plays an important role in the pathogenesis of atopic dermatitis. PMID: 27884290
  6. CD4(+) CD25(+) GARP(+) Treg cells are defective in dilated cardiomyopathy patients and GARP seems to be a better molecular definition of the regulatory phenotype. PMID: 28207945
  7. these results define the oncogenic effects of the GARP-TGFbeta axis in the tumor microenvironment PMID: 27913437
  8. LRRC32 expression is significantly upregulated in human masticatory mucosa during wound healing PMID: 28005267
  9. GARP deficiency leads to accumulation of sphingolipid synthesis intermediates, changes in sterol distribution, and lysosomal dysfunction. PMID: 26357016
  10. since GARP functions as a transporter of transforming growth factor beta (TGFbeta), a cytokine with broad pleiotropic traits, GARP transcriptional attenuation by alternative promoters might provide a mechanism regulating peripheral TGFb PMID: 26584734
  11. GARP is regulated by miRNAs and controls latent TGF-beta1 production by human regulatory T cells. PMID: 24098777
  12. we investigated in detail miR-142-3 pregulation of GARP expression in regulatory CD25(+) CD4 T cells PMID: 23650616
  13. Findings support the idea that GARP is a new latent TGFbeta-binding protein that regulates the bioavailability of TGFbeta and provides a cell surface platform for alphaV integrin-dependent TGFbeta activation. PMID: 22278742
  14. There are 2 independent signals, one in C11orf30 and the other in LRRC32, that are strongly associated with serum IgE levels. C11orf30-LRRC32 region may represent a common locus for atopic diseases via pathways involved in regulation of serum IgE levels PMID: 22070912
  15. GARP is a key receptor controlling FOXP3 in T(reg) cells following T-cell activation in a positive feedback loop assisted by LGALS3 and LGMN PMID: 19453521
  16. the processing and expression of LRRC32 PMID: 21615933
  17. on chromosome 11q13.5 near the leucine-rich repeat containing 32 gene (LRRC32, also known as GARP) associated with asthma risk PMID: 21907864
  18. GARP is a regulatory T cell specific cell surface molecule that mediates suppressive signals and induces Foxp3 expression PMID: 18628982
  19. Platelets and activated Tregs co-express latent TGF-beta and GARP on their membranes. PMID: 19651619
  20. Expression of GARP on activated regulatory T cells correlates with their suppressive capacity. PMID: 19666573
  21. Data show that latent TGF-beta, i.e. both LAP and mature TGF-beta, binds to GARP, which is present on the surface of stimulated Treg clones but not on Th clones. PMID: 19750484

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Subcellular Location
Cell membrane; Single-pass type I membrane protein. Cell surface.
Tissue Specificity
Preferentially expressed in regulatory T-cells (Tregs).
Database Links

HGNC: 4161

OMIM: 137207

KEGG: hsa:2615

STRING: 9606.ENSP00000260061

UniGene: Hs.151641

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