Recombinant Human Macrophage metalloelastase (MMP12)

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Code CSB-EP014659HU
MSDS
Size $224
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP014659HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) MMP12.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP014659HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) MMP12.
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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
MMP12
Uniprot No.
Research Area
Cancer
Alternative Names
EC 3.4.24.65; HME; Macrophage elastase; Macrophage metalloelastase; Macrophage metaloelastase; Matrix metallopeptidase 12 (macrophage elastase); Matrix metalloprotease 12; Matrix metalloproteinase-12; ME; MGC138506; MME; MMP 12; MMP-12; Mmp12; MMP12_HUMAN
Species
Homo sapiens (Human)
Source
E.coli
Expression Region
106-470aa
Target Protein Sequence
GPVWRKHYITYRINNYTPDMNREDVDYAIRKAFQVWSNVTPLKFSKINTGMADILVVFARGAHGDFHAFDGKGGILAHAFGPGSGIGGDAHFDEDEFWTTHSGGTNLFLTAVHEIGHSLGLGHSSDPKAVMFPTYKYVDINTFRLSADDIRGIQSLYGDPKENQRLPNPDNSEPALCDPNLSFDAVTTVGNKIFFFKDRFFWLKVSERPKTSVNLISSLWPTLPSGIEAAYEIEARNQVFLFKDDKYWLISNLRPEPNYPKSIHSFGFPNFVKKIDAAVFNPRFYRTYFFVDNQYWRYDERRQMMDPGYPKLITKNFQGIGPKIDAVFYSKNKYYYFFQGSNQFEYDFLLQRITKTLKSNSWFGC
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
46.1kDa
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description

Boost your cancer research with our premium Recombinant Human MMP12 protein. Macrophage metalloelastase (MMP12), also known as MME or macrophage elastase, is a matrix metalloproteinase that plays a crucial role in extracellular matrix remodeling, tissue repair, and immune response. With implications in the progression of various cancers, MMP12 is a key target for understanding tumor invasion, angiogenesis, and metastasis.

Our Recombinant Human MMP12 protein has been meticulously produced in E. coli to guarantee optimal quality and performance. The protein sequence represents the full length of the mature protein (106-470 amino acids), ensuring that the native structure and functionality are retained. The N-terminal 6xHis-tag enables efficient purification while maintaining the integrity of the protein's activity. With a purity greater than 90% as determined by SDS-PAGE, our Recombinant Human MMP12 protein is the ideal choice for your research in the field of cancer biology.

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Target Background

Function
May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3.
Gene References into Functions
  1. Studied association of matrix metallopeptidase 12 (MMP12) -82 A>G promoter single nucleotide polymorphism (SNP) with bronchial asthma in a Bulgarian population. Results suggest the variant G allele of the MMP12 -82 A>G promoter polymorphism might considered protective for development of bronchial asthma. PMID: 29390099
  2. that MMP-12 might act as a potential target for the development of novel therapeutics of esophageal squamous cell carcinoma PMID: 29033183
  3. MMP-12 (-82A/G) polymorphisms associate with obesity risk and its severity. PMID: 29317790
  4. Significant association observed for MMP-12 (-1082 A/G) (OR 2.55; 95% CI 1.75 to 3.71) gene polymorphism and risk of ischemic stroke. [meta-analysis] PMID: 29906531
  5. The MMP12 down regulation by increasing oxygen concentration enables temporal expression control of MMP12 and involves several mechanisms including HIF-1alpha. These findings suggest MMP12 involved in trophoblast invasion during the first trimester. PMID: 28990117
  6. In the present report, hypoxia is shown to activate a HIF-KDM3A-MMP12 signaling cascade that promotes trophoblast invasion and trophoblast-directed uterine spiral artery remodeling. PMID: 27807143
  7. The current results suggest that MMP12 rs652438 but not MMP12 rs2276109 may affect the risk for Parkinson Disease PMID: 28655442
  8. M4 macrophages are a possible source for HDAC9 and MMP12 expression in advanced human carotid plaques. PMID: 28343758
  9. MMP12 levels were increased in gingival tissue of periodontitis patients. MMP12-producing monocyte-derived cells also had reduced CD200R surface levels. MMP12 production by these cells was induced by CSF2, and treatment with a CD200R ligand reduced CSF2-induced MMP12 production. This novel association of the CD200/CD200R pathway with MMP12 production by monocyte-derived cells may play a key role in PD progression. PMID: 29101312
  10. G allele of MMP12 -82 A > G promoter polymorphism as a protective factor for COPD in Bulgarian population PMID: 28692348
  11. We also found that MMP12 facilitated type I collagen induced platelet aggregation, adhesion and alpha granule secretion. Similarly, one short peptide, WYKG, facilitated type I collagen induced platelet alpha granule secretion. We conclude that platelet express MMP12 may facilitate platelet activation through shedding of CEACAM1. PMID: 28385529
  12. To conclude, Pseudomonas aeruginosa infection induced the expression of matrix metalloproteinases, and Pseudomonas aeruginosa type III secretion system appeared to be a key player in MMP-12 and MMP-13 expression, which is further controlled by NF-kappaB signaling. PMID: 27377745
  13. This study demonstrated that the MMP-12 levels was significantly higher in patients with atheromatous plaques than in those without plaques. PMID: 27345464
  14. High MMP12 expression is associated with gastric cancer. PMID: 28098914
  15. Study evaluated MMP-12 and TIMP-1, TIMP-2, TIMP-3, and TIMP-4 levels in 40 patients with asymptomatic and symptomatic critical carotid artery stenosis (CAS) with neurologic symptoms onset within the preceding 12 hours; results suggest that MMP-12 is related to critical CAS independently on symptoms, moreover, TIMP-3 and TIMP-4 seem to be specifically related to stroke PMID: 27746079
  16. MMP12 causes arterial stiffening in mice and suggest that it functions similarly in humans. PMID: 26608672
  17. Productive complex between MMP-12 and synthetic triple-helical collagen V, are revealed through paramagnetic NMR. PMID: 26887942
  18. These results proved that MMP-12 expression was increased in cutaneous melanoma and associated with tumor progression. It also provided the first evidence that MMP-12 level could be an independent prognostic marker for patients with cutaneous melanoma PMID: 26040769
  19. fluid shear stress induces the synthesis of Insulin growth factor-2 and vascular endothelial growth factor (VEGF) B and D, which in turn transactivate MMP-12. PMID: 25435370
  20. AG and GG genotypes of the MMP12 rs2276109 variant were significantly under-represented among patients with non-contact anterior cruciate ligament rupture compared to controls. PMID: 21410539
  21. HnRNP K can induce MMP12 expression and enzyme activity through activating MMP12 promoter, which promotes cell migration and invasion in nasopharyngeal carcinoma cells. PMID: 24885469
  22. We identified a novel association with an MMP12 locus in large artery atherosclerosis (rs660599; p = 2.5x10), with independent replication in a second population PMID: 25078452
  23. Thrombin-activated microglia expression of tumor necrosis factor-a, interleukin-1b and MMP-12 was significantly reduced through contact-dependent and paracrine mechanisms when the HAESCs were co-cultured with microglia cells PMID: 24424266
  24. Macrophage MMP12 regulates adipose tissue expansion, insulin sensitivity, and expression of inducible nitric oxide synthase. PMID: 24914938
  25. MMP12 is located in genes previously associated with chronic obstructive pulmonary disease susceptibility. PMID: 25006744
  26. In acute aortic dissection patients, macrophage activation is strongly related to the clinical outcome of atherosclerotic disease and the role of MMP-12 as a specific marker. PMID: 24432723
  27. In emphysema, MMP12 mRNA expression was inversely related to the diffusing capacity of the lung. PMID: 23900981
  28. These findings suggest that inhibiting extracellular MMP-12 could be a new avenue for the development of antiviral treatments PMID: 24784232
  29. role of matrix metalloproteinase 12 in non-small cell lung cancer PMID: 24398311
  30. MMP-12 proteolytic activity exists within the aorta specimens and blood samples from aortic dissection patients. PMID: 23642232
  31. The present study evaluated the association between these single nucleotide polymorphisms in MMP12 and MMp13 in patients with colorectal cancer (CRC) patients and healthy controls. PMID: 23898086
  32. MMP-12 expression was found to be significantly associated with extracapsular spread and correlated with nodal metastasis in head and neck squamous cell carcinoma. No relation was found between MMP expression and survival. PMID: 22907031
  33. the endogenous matrix metalloprotease-12 active form can be detected with a novel broad spectrum activity-based probe PMID: 23271741
  34. confers susceptibility to asthma and is associated with asthma severity in a Japanese population PMID: 23075521
  35. Suggest that the inter-individual variability in the MMP-12 gene variation may not be a risk factor for vulnerable plaques in the Chinese Han population. PMID: 22863605
  36. Studied the catalytic domain of MMP-12 in complex with the known inhibitor CGS27023A. A 3D (15)N-edited/(14)N,(12)C-filtered ROESY experiment was used to monitor and distinguish protein amide protons in fast exchange with bulk water. PMID: 22528292
  37. Our results establish microglia as a significant source of progranulin(PGRN), and MMP-12 as modulators of PGRN PMID: 22509390
  38. analysis of orally potent and selective small molecule inhibitor of MMP-12 for treatment of COPD and asthma in human and mouse models PMID: 22153340
  39. Sputum MMP-12 concentrations and activity in patients with chronic obstructive pulmonary disease are associated with the extent of emphysema PMID: 22305682
  40. MMP-12 participates in experimental small airway modelling and in emphysema [review] PMID: 21920892
  41. Provide in vivo evidence of an effect of MMP12 on fibrinogen level. PMID: 22119538
  42. Surfaces of MMP-12 far from its active site have been found to affect degradation and affinity for elastin substrates; main exosite 1 has been extended to Asp-124 that binds calcium while exosite 2 comprises residues near the back of the catalytic domain. PMID: 21967233
  43. rs652438 SNP alters MMP-12 activity with the A allele being more active, which is associated with increased macrophage infiltration and emphysema in the lungs of patients with COPD. These findings further implicate MMP-12 and this SNP in COPD PMID: 21730350
  44. MMP-12 mRNA may be a valuable prognostic marker for both overall survival and tumour recurrence of hepatocellular patients after liver resection. PMID: 21683576
  45. High expression of MMP-12 in squamous laryngeal carcinoma was related to significant hyperplasia. PMID: 17357518
  46. Data suggest that the matrix metalloproteinase-12 rs2276109 gene polymorphism may contribute to susceptibility to systemic sclerosis (SSc), and in particular to diffuse cutaneous SSc and pulmonary fibrosis. PMID: 20595276
  47. Overexpression of human macrophage metalloelastase is strongly correlated with the reduced angiogenesis and vascular invasion of gastric carcinoma, and may serve as a useful predictive indicator in patients with this disease. PMID: 20127415
  48. distribution of N357S genotypes not significantly different in chronic obstructive pulmonary disease PMID: 20160424
  49. Individuals with MMP-12 -82A/G and MMP-13 -77A/A might have higher risk of overall or special histological type of epithelial ovarian carcinoma development. PMID: 20376807
  50. The catalytic domain of MMP-12 binds to the triple helix and cleaves the typical sites -Gly(775)-Leu(776)- in alpha-2 type I collagen and -Gly(775)-Ile(776)- in alpha-1 type I and type III collagens and at multiple other sites in both collagen types. PMID: 19932771

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Subcellular Location
Secreted, extracellular space, extracellular matrix.
Protein Families
Peptidase M10A family
Tissue Specificity
Found in alveolar macrophages but not in peripheral blood monocytes.
Database Links

HGNC: 7158

OMIM: 601046

KEGG: hsa:4321

UniGene: Hs.1695

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