Recombinant Human Mannosyl-oligosaccharide 1,2-alpha-mannosidase IA (MAN1A1), partial,Yeast

Code CSB-YP013383HU1
MSDS
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
MAN1A1
Uniprot No.
Alternative Names
MAN1A1; Mannosyl-oligosaccharide 1,2-alpha-mannosidase IA; Man(9-alpha-mannosidase; Man9-mannosidase; Mannosidase alpha class 1A member 1; Processing alpha-1,2-mannosidase IA; Alpha-1,2-mannosidase IA
Species
Homo sapiens (Human)
Source
Yeast
Protein Length
Full length protein
Tag Info
N-terminal His-tagged
Tag-Free
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

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Target Background

Function
Involved in the maturation of Asn-linked oligosaccharides. Progressively trim alpha-1,2-linked mannose residues from Man(9)GlcNAc(2) to produce Man(5)GlcNAc(2).
Gene References into Functions
  1. In situ proximity ligation assays of Golgi localization of alpha-mannosidase IA at giantin versus GM130-GRASP65 site, and absence or presence of N-glycans terminated with alpha3-mannose on trans-Golgi glycosyltransferases may be useful for distinguishing indolent from aggressive prostate cancer cells PMID: 28782625
  2. We observed significant lower values of GAL, FUC and tendency to decrease of alpha-mannosidase and GLU concentration in nasal polyps PMID: 23911047
  3. Complex N-glycans generated by enzymes such as a-mannosidase I inhibit the activation of naive T cells. PMID: 21577142
  4. overexpression of Golgi alpha1,2-mannosidase IA, IB, and IC also accelerates ERAD of terminally misfolded human alpha1-antitrypsin variant null (Hong Kong) (NHK), and mannose trimming from the N-glycans on NHK in 293 cells PMID: 17727818
  5. following HIV infection, there is an increased rate of catabolism of glycoconjugates in saliva resulting from changes in the proportions of the activity of isoenzymes A and B of N-acetyl-beta-hexosaminidase, beta-galactosidase and alpha-fucosidase PMID: 18217416
  6. ERManI, by functioning as a downstream effector target of EDEM1, represents a checkpoint activation paradigm by which the mammalian unfolded protein response coordinates the boosting of endoplasmic reticulum (ER)-associated degradation. PMID: 19258393

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Subcellular Location
Golgi apparatus membrane; Single-pass type II membrane protein.
Protein Families
Glycosyl hydrolase 47 family
Database Links

HGNC: 6821

OMIM: 604344

KEGG: hsa:4121

STRING: 9606.ENSP00000357453

UniGene: Hs.102788

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