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| Code | CSB-BP013466HU |
| Product Type | Recombinant Protein |
| Size | US$250 |
| Uniprot No. | P45983 |
| Relevance | Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1. In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins. Loss of this interaction abrogates the acetylation required for replication initiation. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including p53/TP53 and Yes-associates protein YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Contributes to the survival of erythroid cells by phosphorylating the antagonist of cell death BAD upon EPO stimulation. Mediates starvation-induced BCL2 phosphorylation, BCL2 dissociation from BECN1, and thus activation of autophagy. Phosphorylates STMN2 and hence regulates microtubule dynamics, controlling neurite elongation in cortical neurons. In the developing brain, through its cytoplasmic activity on STMN2, negatively regulates the rate of exit from multipolar stage and of radial migration from the ventricular zone. Phosphorylates several other substrates including heat shock factor protein 4 (HSF4), the deacetylase SIRT1, ELK1, or the E3 ligase ITCH. Phosphorylates the CLOCK-ARNTL/BMAL1 heterodimer and plays a role in the regulation of the circadian clock (PubMed:22441692). |
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| Storage Buffer | Tris-based buffer,50% glycerol |
| Alias | JNK-46 Stress-activated protein kinase 1c Short name: SAPK1c Stress-activated protein kinase JNK1 c-Jun N-terminal kinase 1 |
| Species | Homo sapiens (Human) |
| Purity | Greater than 90% as determined by SDS-PAGE. |
| Sequence | SKRDNNFYSVEIGDSTFTVLKRYQNLKPIGSGAQGIVCAAYDAILERNVAIKKLSRPFQNQTHAKRAYRELVLMKCVNHKNIIGLLNVFTPQKSLEEFQDVYIVMELMDANLCQVIQMELDHERMSYLLYQMLCGIKHLHSAGIIHRDLKPSNIVVKSDCTLKILDFGLARTAGTSFMMTPYVVTRYYRAPEVILGMGYKENVDLWSVGCIMGEMVCHKILFPGRDYIDQWNKVIEQLGTPCPEFMKKLQPTVRTYVENRPKYAGYSFEKLFPDVLFPADSEHNKLKASQARDLLSKMLVIDASKRISVDEALQHPYINVWYDPSEAEAPPPKIPDKQLDEREHTIEEWKELIYKEVMDLEERTKNGVIRGQPSPLGAAVINGSQHPSSSSSVNDVSSMSTDPTLASDTDSSLEAAAGP |
| Research Area | Cancer |
| Source | Baculovirus |
| Gene Names | MAPK8 |
| Expression Region | 4-422aa |
| Tag Info | N-terminal 6xHis-tagged |
| Mol. Weight | 49.4kDa |
| Protein Description | Partial |
| Storage | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. |
| Notes | Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
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| Function | Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1. In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins. Loss of this interaction abrogates the acetylation required for replication initiation. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including p53/TP53 and Yes-associates protein YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Contributes to the survival of erythroid cells by phosphorylating the antagonist of cell death BAD upon EPO stimulation. Mediates starvation-induced BCL2 phosphorylation, BCL2 dissociation from BECN1, and thus activation of autophagy. Phosphorylates STMN2 and hence regulates microtubule dynamics, controlling neurite elongation in cortical neurons. In the developing brain, through its cytoplasmic activity on STMN2, negatively regulates the rate of exit from multipolar stage and of radial migration from the ventricular zone. Phosphorylates several other substrates including heat shock factor protein 4 (HSF4), the deacetylase SIRT1, ELK1, or the E3 ligase ITCH. Phosphorylates the CLOCK-ARNTL/BMAL1 heterodimer and plays a role in the regulation of the circadian clock |
| Subcellular Location | Cytoplasm, Nucleus |
| Protein Families | Protein kinase superfamily, CMGC Ser/Thr protein kinase family, MAP kinase subfamily |
| Database Links |
HGNC: 6881 OMIM: 601158 KEGG: hsa:5599 STRING: 9606.ENSP00000353483 UniGene: Hs.138211 |
| Pathway |
cAMP signaling pathway
ErbB signaling pathway MAPK signaling pathway Wnt signaling pathway Focal adhesion TNF signaling pathway Apoptosis Autophagy Necroptosis Protein processing in endoplasmic reticulum Ras signaling pathway Fc epsilon RI signaling pathway IL-17 signaling pathway NOD-like receptor signaling pathway Osteoclast differentiation Th1 and Th2 cell differentiation Th17 cell differentiation Toll-like receptor signaling pathway Adipocytokine signaling pathway AGE-RAGE signaling pathway in diabetic complications ER Stress pathway FoxO signaling pathway Insulin signaling pathway Neurotrophin signaling pathway |
MAPK8 Antibodies for Homo sapiens (Human)
| Code | Product Name | Species Reactivity | Application |
|---|---|---|---|
| CSB-PA013466GA01HU | MAPK8 Antibody | Human,Mouse,Rat | ELISA,WB,IHC |
| CSB-PA222139 | MAPK8 Antibody | Human,Mouse,Rat | ELISA,WB |
| CSB-PA236755 | MAPK8 Antibody | Human,Mouse,Rat | ELISA,WB |
| CSB-PA176985 | MAPK8 Antibody | Human,Rat | ELISA,WB,IHC |
| CSB-PA282702 | MAPK8 Antibody | Human,Mouse,Rat | ELISA,WB,IHC |
MAPK8 Antibodies for Human
| Code | Product Name | Species Reactivity | Application |
|---|---|---|---|
| CSB-PA16759A0Rb | MAPK8 Antibody | Human | ELISA, WB, IHC, IF |
| CSB-PA16759B0Rb | MAPK8 Antibody, HRP conjugated | Human | ELISA |
| CSB-PA16759C0Rb | MAPK8 Antibody, FITC conjugated | Human | ELISA |
| CSB-PA16759D0Rb | MAPK8 Antibody, Biotin conjugated | Human | ELISA |
MAPK8 Proteins for Homo sapiens (Human)
| Code | Product Name | Source |
|---|---|---|
| CSB-YP013466HU CSB-EP013466HU CSB-MP013466HU |
Recombinant Human Mitogen-activated protein kinase 8(MAPK8) | Yeast E.coli Mammalian cell |
Recombinant Mouse Nuclear respiRatory factor 1(Nrf1)
Express system: Yeast
Species: Mus musculus (Mouse)
Recombinant Human Glutathione S-transferase kappa 1(GSTK1)
Express system: E.coli
Species: Homo sapiens (Human)
Recombinant Macaca mulatta Growth hormone receptor(GHR),partial
Express system: E.coli
Species: Macaca mulatta (Rhesus macaque)
Recombinant Mouse Integrin alpha-L(Itgal),partial
Express system: E.coli
Species: Mus musculus (Mouse)
Recombinant Mouse L-dopachrome tautomerase(Dct),partial
Express system: E.coli
Species: Mus musculus (Mouse)
Recombinant Human Calcium-dependent phospholipase A2(PLA2G5)
Express system: Yeast
Species: Homo sapiens (Human)
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