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Mitogen-activated protein kinase 8 is a protein in humans that is encoded by MAPK8 gene. Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1. In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins.
The following MAPK8 reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.
MAPK8 Antibodies for Homo sapiens (Human)
| Code | Product Name | Species Reactivity | Application |
|---|---|---|---|
| CSB-PA013466GA01HU | MAPK8 Antibody |
Human,Mouse,Rat | ELISA,WB,IHC |
| CSB-PA222139 | MAPK8 Antibody |
Human,Mouse,Rat | ELISA,WB |
| CSB-PA236755 | MAPK8 Antibody |
Human,Mouse,Rat | ELISA,WB |
| CSB-PA176985 | MAPK8 Antibody |
Human,Rat | ELISA,WB,IHC |
| CSB-PA282702 | MAPK8 Antibody |
Human,Mouse,Rat | ELISA,WB,IHC |
| CSB-PA16759A0Rb | MAPK8 Antibody |
Human | ELISA, WB, IHC, IF |
| CSB-PA16759B0Rb | MAPK8 Antibody, HRP conjugated |
Human | ELISA |
| CSB-PA16759D0Rb | MAPK8 Antibody, Biotin conjugated |
Human | ELISA |
MAPK8 Antibodies for Danio rerio (Zebrafish) (Brachydanio rerio)
| Code | Product Name | Species Reactivity | Application |
|---|---|---|---|
| CSB-PA013466XA01DIL | mapk8 Antibody |
Danio rerio (Zebrafish) (Brachydanio rerio) | ELISA, WB (ensure identification of antigen) |
MAPK8 Proteins for Homo sapiens (Human)
| Code | Product Name | Source |
|---|---|---|
| CSB-YP013466HU CSB-EP013466HU CSB-MP013466HU CSB-EP013466HU-B |
Recombinant Human Mitogen-activated protein kinase 8(MAPK8) |
Yeast E.coli Mammalian cell In Vivo Biotinylation in E.coli |
| CSB-BP013466HU | Recombinant Human Mitogen-activated protein kinase 8(MAPK8),partial |
Baculovirus |
MAPK8 Proteins for Rattus norvegicus (Rat)
| Code | Product Name | Source |
|---|---|---|
| CSB-YP013466RA CSB-EP013466RA CSB-BP013466RA CSB-MP013466RA CSB-EP013466RA-B |
Recombinant Rat Mitogen-activated protein kinase 8(Mapk8) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
MAPK8 Proteins for Xenopus laevis (African clawed frog)
| Code | Product Name | Source |
|---|---|---|
| CSB-YP013466XBE CSB-EP013466XBE CSB-BP013466XBE CSB-MP013466XBE CSB-EP013466XBE-B |
Recombinant Xenopus laevis Mitogen-activated protein kinase 8(mapk8) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
MAPK8 Proteins for Mus musculus (Mouse)
| Code | Product Name | Source |
|---|---|---|
| CSB-YP013466MO CSB-EP013466MO CSB-BP013466MO CSB-MP013466MO CSB-EP013466MO-B |
Recombinant Mouse Mitogen-activated protein kinase 8(Mapk8) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
MAPK8 Proteins for Danio rerio (Zebrafish) (Brachydanio rerio)
| Code | Product Name | Source |
|---|---|---|
| CSB-YP013466DIL CSB-EP013466DIL CSB-BP013466DIL CSB-MP013466DIL CSB-EP013466DIL-B |
Recombinant Danio rerio Mitogen-activated protein kinase 8(mapk8) |
Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
The MAPK8 gene encodes mitogen-activated protein kinase 8, an enzyme also known as JNK1 [1][2]. JNK1 is ubiquitously expressed [2]. As a serine/threonine-protein kinase, MAPK8/JNK1 is involved in various processes such as cell proliferation, differentiation, migration, transformation, and apoptosis. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAPK/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1.
In turn, MAPK8/JNK1 phosphorylates many transcription factors, primarily components of AP-1 such as JUN, JDP2, and ATF2 and thus regulates AP-1 transcriptional activity, eventually inducing cell proliferation, differentiation and stress responses [3-6]. Recently, it was demonstrated that JNK1 signaling plays a crucial role in the central nervous system (CNS) and in the pituitary to control systemic glucose and lipid metabolism partially through regulation of hormones involved in growth and energy expenditure [7].
[1] Dérijard B, Hibi M, et al. JNK1: a protein kinase stimulated by UV light and Ha-Ras that binds and phosphorylates the c-Jun activation domain [J]. Cell. 1994, 76 (6): 1025–37.
[2] Davis RJ. Signal transduction by the JNK group of MAP kinases [J]. Cell 2000;103:239–252.
[3] Gupta S, Barrett T, et al. Selective interaction of JNK protein kinase isoforms with transcription factors [J]. EMBO J. 1996, 15 (11): 2760–70.
[4] Dérijard B, Hibi M, et al. JNK1: a protein kinase stimulated by UV light and Ha-Ras that binds and phosphorylates the c-Jun activation domain [J]. Cell 1994, 76: 1025–1037.
[5] Kyriakis J, Banerjee P, The stress-activated protein kinase subfamily of c-Jun kinases [J]. Nature 1994, 369: 156–160.
[6] Nishina H, Wada T, et al. Physiological roles of SAPK/JNK signaling pathway [J]. J Biochem. 2004 Aug;136(2):123-6.
[7] Belgardt BF1, Mauer J, et al. Novel roles for JNK1 in metabolism [J]. Aging (Albany NY). 2010 Sep;2(9):621-6.
