Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

ABCC1

Uniprot No.

P33527

Research Area

Signal Transduction

Alternative Names

ABC 29; ABC29; ABCC 1; ABCC; Abcc1; ATP binding cassette sub family C (CFTR/MRP) member 1; ATP binding cassette sub-family C member 1; ATP binding cassette subfamily C member 1; ATP binding cassette transporter variant ABCC1delta ex13; ATP binding cassette transporter variant ABCC1delta ex13&14; ATP binding cassette transporter variant ABCC1delta ex25; ATP binding cassette transporter variant ABCC1delta ex25&26; ATP binding cassette, sub-family C (CFTR/MRP), member 1; ATP-binding cassette sub-family C member 1; DKFZp686N04233; DKFZp781G125; GS X; GSX; Leukotriene C(4) transporter; LTC4 transporter; MRP 1; MRP; MRP1; MRP1_HUMAN; Multidrug resistance associated protein 1; Multidrug resistance protein; Multidrug resistance-associated protein 1; Multiple drug resistance associated protein; Multiple drug resistance protein 1

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

1248-1531aa

Target Protein Sequence

VRMSSEMETNIVAVERLKEYSETEKEAPWQIQETAPPSSWPQVGRVEFRNYCLRYREDLDFVLRHINVTINGGEKVGIVGRTGAGKSSLTLGLFRINESAEGEIIIDGINIAKIGLHDLRFKITIIPQDPVLFSGSLRMNLDPFSQYSDEEVWTSLELAHLKDFVSALPDKLDHECAEGGENLSVGQRQLVCLARALLRKTKILVLDEATAAVDLETDDLIQSTIRTQFEDCTVLTIAHRLNTIMDYTRVIVLDKGEIQEYGAPSDLLQQRGLFYSMA kDaGLV
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

35.9 kDa

Protein Length

Partial

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The production of this recombinant Human ABCC1 protein is just like all recombinant proteins. The process involved transfecting E.coli cells with DNA vector containing the template of recombinant DNA. The E.coli cells containing the template were then cultured so that they could transcribe and translate the ABCC1 protein. N-terminal 6xHis tag was used in the process. The purity is 0.9 determined by SDS-PAGE.

Multidrug resistance-associated protein 1 (MRP1/ABCC1) is a member of the ATP-binding cassette (ABC) transporter superfamily which contains 49 members in human that are divided into 7 subfamilies, named from ABCA to ABCG. (MRP1/ABCC1 is the first identified member of ABCC subfamily which belongs to ATP-binding cassette (ABC) transporter superfamily. It is ubiquitously expressed in almost all human tissues and transports a wide spectrum of substrates including drugs, heavy metal anions, toxicants, and conjugates of glutathione, glucuronide and sulfate. With the advance of sequence technology, many MRP1/ABCC1 polymorphisms have been identified. Accumulating evidences show that some polymorphisms are significantly associated with drug resistance and disease susceptibility. Recent advances showed the role and mechanism of MRP1/ABCC1 polymorphisms in drug resistance, toxicity, disease susceptibility and severity, prognosis prediction, and methods to select and predict functional polymorphisms.

Target Background

Function

Mediates export of organic anions and drugs from the cytoplasm. Mediates ATP-dependent transport of glutathione and glutathione conjugates, leukotriene C4, estradiol-17-beta-o-glucuronide, methotrexate, antiviral drugs and other xenobiotics. Confers resistance to anticancer drugs by decreasing accumulation of drug in cells, and by mediating ATP- and GSH-dependent drug export. Hydrolyzes ATP with low efficiency. Catalyzes the export of sphingosine 1-phosphate from mast cells independently of their degranulation. Participates in inflammatory response by allowing export of leukotriene C4 from leukotriene C4-synthezing cells.

Gene References into Functions

Study suggest that increase in MRP1 gene expression is associated with being a nonsmoker, adenocarcinoma, advanced clinical stages and a poor response to chemotherapy in patients with nonsmall cell lung cancer.[meta-analysis] PMID: 30132569
Cysteine residues were introduced into TMs 8, 14, 15, and 16 of 3Cys DeltaMRP1. PMID: 29572332
Efflux of different arsenic metabolites by MRP1 is likely influenced by multiple factors. PMID: 29752257
Peripheral blood MRP1 expression is increased in patients with refractory epilepsy PMID: 26099600
Due to the downregulation of MDR1 and MRP1, the intracellular accumulation of ADM was increased in the transfected cells compared with that in the parental K562/ADM cells..Our research revealed that protein expression of the ERK signaling pathway was inhibited by downregulating TRIB2, indicating that the ERK pathway was involved in cell drug resistance and proliferation. PMID: 29436678
icaritin (ICT) decreased the mRNA and protein levels of multidrug resistance-associated protein 1 (MRP1) in MG-63 doxorubicin-resistant (MG-63/DOX) cells. PMID: 29425587
Enhanced BRAF-mediated NRF2 gene transcription and Histone Acetyltransferases-mediated NRF2 protein acetylation contributes to ABCC1-mediated chemoresistance and glutathione-mediated survival in acquired topoisomerase II poison-resistant cancer cells. PMID: 29080842
Pomolic acid also down-modulated the activity of the multidrug resistance associated protein 1 (MRP1) and inhibited migration of Glioblastoma (GBM) cells. These results show that PA acts on several pathways of GBM drug resistance and therefore may be of potential interest for the treatment of this tumor PMID: 28849227
Our data present for the very first time the high negative correlation between MRP1 protein expression in LSC and AML patients' remission. It does strongly suggest that MRP1 expression in LSC is an adverse prognostic marker in patients with de novo AML. PMID: 28805931
No significant correlation was observed between rs504348 genotypes and cystic fibrosis severity. A significantly higher expression of ABCC1 mRNA was induced by CF plasma compared to healthy control plasma. PMID: 28800122
MRP1 is regulated by CLIC1, leading to CLIC1-mediated development of chemoresistance in choriocarcinoma cells. PMID: 27983917
High MRP1 expression is associated with resistance to doxorubicin in breast cancer. PMID: 27878697
Our data indicates that hypomethylation of the ABCC1 promoter is not cancer type-specific but occurs in cancer cell lines of different origins. PMID: 27689338
Our work demonstrates for the first time that the adaptation to endoplasmic reticulum(ER) stress in cancer cells produces a MDR phenotype. The PERK/Nrf2/MRP1 axis is responsible for the resistance to ER stress and chemotherapy, and may represent a good therapeutic target in aggressive and resistant tumors. PMID: 28499449
Data show that miR-145 level decreased in breast cancer tissues, breast cancer cell lines and doxorubicin resistant MCF-7 cells, and inversely correlated with multidrug resistance-associated protein 1 (MRP1) expression level. PMID: 27487127
PG accumulation assay is a new, unique tool for the parallel determination of the function of the ABCG2, ABCB1, and ABCC1 multidrug transporters PMID: 29342177
ABCC1 exports corticosterone but not cortisol. We show that ABCC1 is expressed in adipose tissue and that ABCC1 inhibition increases intracellular corticosterone, but not cortisol, and induces glucocorticoid-responsive gene transcription in adipocytes. PMID: 27535620
GSK1904529A did not alter the expression level of MRP1, induced a G0/G1 phase cell cycle arrest. Our results indicated that GSK1904529A significantly increased the sensitivity of MRP1 overexpressing cells to chemotherapeutic agents. PMID: 28266043
High MRP1 expression is associated with ovarian cancer. PMID: 27739315
These results show that multidrug resistant leukemia cell microparticle regulation of ABCC1 in recipient cells is governed by the transfer of both miR-326 and ABCB1 from donor cells PMID: 28050891
Higher expression of ABCG2 and MRP1, and lower expression of CK19 in papillary thyroid carcinomas with a solid component were associated with higher tumor recurrence rate and shorter disease-free survival. PMID: 28411177
Visfatin increases the nuclear translocation of Akt and its binding with ABCC1. These data showed that visfatin can decrease doxorubicin sensitivity of Non-small-cell lung cancer cells via activation of Akt/MRP1. PMID: 28762597
Studied hedgehog signaling effect thru ATP-binding cassette (ABC) transporters in hepatoma drug sensitivity. Results suggest Hedgehog signaling transcription factor Gli-2 to be the primary regulator for drug sensitivity of hepatoma through the ABCC1 transporter. PMID: 28414325
This study demonstrated that mitochondrial MRP-1 is expressed in the outer mitochondrial membrane and is a client protein of HSP90beta. PMID: 26722004
High ABCC1 expression is associated with hepatic fibrosis. PMID: 28098912
This study shows direct binding between MRP1 Linker 1 (L1MRP1) domain and alpha- and beta-tubulin subunits, using a scanning peptide approach PMID: 27908733
In summary, chaetominine strongly reverses drug resistance by interfering with the PI3K/Akt/Nrf2 signaling, resulting in reduction of MRP1-mediated drug efflux and induction of Bax/Bcl-2-dependent apoptosis in an ADR-resistant K562/Adr leukemia cell line. PMID: 27038543
cross-talk between MRP1 glycosylation and phosphorylation occurs. PMID: 27297967
meta-analysis results strongly suggest that MRP1 is overexpressed in both neurons and astrocytes of intractable epilepsy patients, inhibition of MRP1 may enhance antiepileptic drug efficacy by increasing local drug availability PMID: 26000815
Our results show MRP1 as a potential biomarker of resistance to treatment with irinotecan when found in CTCs from mCRC patients. PMID: 26950035
Taxus chinensis var.-mediated downregulation of MRP1, MDR1, and LRP might contribute to the reversal of drug resistance in non-small cell lung cancer stem cells. PMID: 27706681
miR-145 accelerated MRP1 mRNA degradation by directly targeting its 3'-UTR and therefore caused increased cisplatin toxicity in gallbladder cancer cells. Lower miR-145 and higher MRP1 expression levels predicted poor prognosis in GBC patients who received chemotherapy. PMID: 26852750
Caveolin-1 affects ESCC MDR by regulating the expressions of P-gp and MRP1. PMID: 26768616
Report ABCC1 induction by genistein in breast cancer cells may result in drug resistance in tumors treated with substrates of this transport. PMID: 27033456
Results suggest that the LRIG1could negatively control MRP-1 and the apoptosis to improve the sensitivity of VP16-related chemotherapy. PMID: 27183435
Data, including data from studies in knockout mice, suggest that MRP1 is involved in development of vascular endothelial dysfunction in diabetic angiopathy; MRP1 appears to be required for oxidative stress in endothelial cells due to hyperglycemia. PMID: 26908299
The expression of ABCB1, ABCC1 and ABCG2 on primary human T-cells correlates with the efficacy of JAK inhibitor ruxolitinib treatment in regard to T-cell activation and proliferation. PMID: 26589910
Positive expressions of MRP and TOP2A in the tumor tissue are associated with increased risk of developing brain metastases in non-small cell lung cancer (NSCLC). PMID: 26617887
the expression and localization of the ATP-Binding Cassette (ABC) transporters BCRP (ABCG2), P-gp (ABCB1) and MRP1 (ABCC1) in human umbilical cords, is reported. PMID: 26407213
Report decreased function MRP1 activity in methotrexate treated rheumatoid arthritis patients. PMID: 26715450
the specific COX-2 inhibitor meloxicam can increase the intracellular accumulation of doxorubicin and enhance doxorubicin-induced cytotoxicity in A549 cancer cells by reducing the expression of MRP1 and MRP4. PMID: 26600514
The scanning study identified 46 polymorphic variants by screening 30 exons of the ABCC1 gene with adjacent intronic fragments in 190 unrelated Polish individuals. A large fraction of variants represented rare or even completely novel ones. PMID: 26395522
ILK may have an important role in the progression of NSCLC, possibly through up-regulation of Snail and MRP1. PMID: 25964055
MRP1 knockdown down-regulates the deposition of collagen and leads to a reduced hypertrophic scar fibrosis PMID: 26092470
MRP1 activity showed no significant change I primary colorectal cancer patients. PMID: 25885226
Chronic arsenic exposure negatively correlates with the expression of MRP1 in bronchoalveolar lavage cells in patients with lung cancer. PMID: 26031227
The present study investigated the time course and dose dependency of the induction of three efflux proteins, P-gp, MRP1 and MRP5, in response to gemcitabine exposure in Capan-2 pancreatic cancer cell line at transcriptional and translational levels. PMID: 25564970
Our data suggested that miR-7 mediated small cell lung cancer chemoresistance by repressing MRP1/ABCC1 PMID: 26108539
study shows that genetic variability in the nucleotide binding domain 1 has a significant impact on the ABCC1 transcript level in the target tissue and may modify survival of breast cancer patients PMID: 25078270
The level of FBW7 expression in CNE2 cells was correlated with CDDP chemosensitivity. siRNA-mediated upregulation of FBW7 expression downregulated the expression of MRP, significantly increasing drug sensitivity in CNE2 cells. PMID: 25586348

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Subcellular Location

Cell membrane; Multi-pass membrane protein.

Protein Families

ABC transporter superfamily, ABCC family, Conjugate transporter (TC 3.A.1.208) subfamily

Tissue Specificity

Lung, testis and peripheral blood mononuclear cells.

Database Links

HGNC: 51

OMIM: 158343

KEGG: hsa:4363

STRING: 9606.ENSP00000382342

UniGene: Hs.391464

Code	CSB-EP001056HU
Abbreviation	Recombinant Human ABCC1 protein, partial
MSDS	MSDS Datasheet
Size	US$256
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Multidrug resistance-associated protein 1 (ABCC1), partial

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