Recombinant Human Myosin-binding protein C, fast-type (MYBPC2), partial

Code CSB-YP618884HU
MSDS
Size $250
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
MYBPC2
Uniprot No.
Research Area
Signal Transduction
Alternative Names
C protein, skeletal muscle fast isoform; C-protein; Fast MyBP C; Fast MyBP-C; MGC163408; MYBPC; Mybpc2; MYBPCF; Myosin binding protein C, fast type; Myosin-binding protein C, fast-type; MYPC2_HUMAN; skeletal muscle fast isoform
Species
Homo sapiens (Human)
Source
Yeast
Expression Region
739-1141aa
Target Protein Sequence
EPLHLIVEDVTDTTTTLKWRPPNRIGAGGIDGYLVEYCLEGSEEWVPANTEPVERCGFTVKNLPTGARILFRVVGVNIAGRSEPATLAQPVTIREIAEPPKIRLPRHLRQTYIRKVGEQLNLVVPFQGKPRPQVVWTKGGAPLDTSRVHVRTSDFDTVFFVRQAARSDSGEYELSVQIENMKDTATIRIRVVEKAGPPINVMVKEVWGTNALVEWQAPKDDGNSEIMGYFVQKADKKTMEWFNVYERNRHTSCTVSDLIVGNEYYFRVYTENICGLSDSPGVSKNTARILKTGITFKPFEYKEHDFRMAPKFLTPLIDRVVVAGYSAALNCAVRGHPKPKVVWMKNKMEIREDPKFLITNYQGVLTLNIRRPSPFDAGTYTCRAVNELGEALAECKLEVRVPQ
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
47.5kDa
Protein Length
Partial
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

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Target Background

Function
Thick filament-associated protein located in the crossbridge region of vertebrate striated muscle a bands. In vitro it binds MHC, F-actin and native thin filaments, and modifies the activity of actin-activated myosin ATPase. It may modulate muscle contraction or may play a more structural role.
Gene References into Functions
  1. the mean methylation level of CpGs was significantly higher in MYBPC3 than MYBPC2. PMID: 20740642
  2. The patients with a frameshift deletion mutation of MyBPC may develop LV systolic dysfunction and suffer from cardiovascular events through mid-life and beyond. PMID: 20605413
  3. Identification of interactions between domains of Myosin binding protein-C that are modulated by hypertrophic cardiomyopathy missense mutations. PMID: 12386147
  4. the CD-loop is a natively unfolded sequence with a likely coupling between folding and ligand binding. PMID: 17876814
  5. Role and quaternary structure of MyBPC in skeletal muscle. PMID: 18201573
  6. The distribution of MyBP-C in the A-bands of cardiac and skeletal muscles and compare this to the A-band structure in cardiac muscle of MyBP-C-deficient mice, was investigated. PMID: 18817784

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Protein Families
Immunoglobulin superfamily, MyBP family
Database Links

HGNC: 7550

OMIM: 160793

KEGG: hsa:4606

STRING: 9606.ENSP00000350332

UniGene: Hs.85937

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