Recombinant Human Myosin light chain 3 (MYL3)

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Code CSB-EP015310HU
Size US$306
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
MYL3
Uniprot No.
Alternative Names
Cardiac myosin light chain 1; CMH8 ; CMLC1; ELC of myosin; Essential light chain of myosin; MLC1SB; MLC1V; MYL3; MYL3_HUMAN; Myosin light chain 1; Myosin light chain 1 slow twitch muscle B/ventricular isoform; Myosin light chain 1 slow, B; Myosin light chain 3; Myosin, light chain 1, ventricular; myosin, light chain 3, alkali, ventricular, skeletal, slow; myosin, light polypeptide 3, alkali; myosin, light polypeptide 3, alkali, ventricular, skeletal, slow; OTTHUMP00000165922; Slow skeletal ventricular myosin alkali light chain 3; slow-twitch muscle B/ventricular isoform; Ventricular/slow twitch myosin alkali light chain; VLC1
Species
Homo sapiens (Human)
Source
E.coli
Expression Region
1-195aa
Target Protein Sequence
MAPKKPEPKKDDAKAAPKAAPAPAPPPEPERPKEVEFDASKIKIEFTPEQIEEFKEAFMLFDRTPKCEMKITYGQCGDVLRALGQNPTQAEVLRVLGKPRQEELNTKMMDFETFLPMLQHISKNKDTGTYEDFVEGLRVFDKEGNGTVMGAELRHVLATLGERLTEDEVEKLMAGQEDSNGCINYEAFVKHIMSS
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
48.8kDa
Protein Length
Full Length
Tag Info
N-terminal GST-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description

The synthesis of the recombinant plasmid containing the gene encoding the Human MYL3 protein (1-195aa) is the first step to produce the recombinant Human MYL3 protein. After that, the recombinant plasmid is transformed into e.coli cells. e.coli cells capable of enduring a specific antibiotic are selected, demonstrating successful uptake of the recombinant plasmid. The e.coli cells containing the recombinant plasmid are cultured under conditions that encourage the expression of the gene of interest. A N-terminal GST tag is linked to the protein. Following expression, affinity purification is employed to isolate and purify the recombinant Human MYL3 protein from the cell lysate. Denaturing SDS-PAGE is applied to resolve the resulting recombinant Human MYL3 protein, indicating a purity level exceeding 90%.

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Target Background

Function
Regulatory light chain of myosin. Does not bind calcium.
Gene References into Functions
  1. In Familial Hypertrophic Cardiomyopathy, the MYL3 Arg94His variant was associated with high disease penetrance and substantial interventricular septal hypertrophy PMID: 26443374
  2. N-fragment is the binding domain of human ventricular LC1, whereas the C-fragment serves as a functional domain, which may be more involved in the modulation of the actin-activated ATPase activity of myosin PMID: 14516912
  3. Cell-permeable peptide containing the 15 amino acid N-terminal peptide from human ventricular light chain-1 (VLC-1) enhanced myocardial contractility. PMID: 17717642
Involvement in disease
Cardiomyopathy, familial hypertrophic 8 (CMH8)
Database Links

HGNC: 7584

OMIM: 160790

KEGG: hsa:4634

STRING: 9606.ENSP00000292327

UniGene: Hs.517939

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