Recombinant Human N-acetylmuramoyl-L-alanine amidase (PGLYRP2)

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Code CSB-YP846684HU
MSDS
Size $250
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of CSB-YP846684HU could indicate that this peptide derived from Yeast-expressed Homo sapiens (Human) PGLYRP2.
  • Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of CSB-YP846684HU could indicate that this peptide derived from Yeast-expressed Homo sapiens (Human) PGLYRP2.
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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
PGLYRP2
Uniprot No.
Research Area
Immunology
Alternative Names
PGLYRP2; PGLYRPL; PGRPL; UNQ3103/PRO10102; N-acetylmuramoyl-L-alanine amidase; EC 3.5.1.28; Peptidoglycan recognition protein 2; Peptidoglycan recognition protein long; PGRP-L
Species
Homo sapiens (Human)
Source
Yeast
Expression Region
22-576aa
Target Protein Sequence
SLPLLMDSVIQALAELEQKVPAAKTRHTASAWLMSAPNSGPHNRLYHFLLGAWSLNATELDPCPLSPELLGLTKEVARHDVREGKEYGVVLAPDGSTVAVEPLLAGLEAGLQGRRVINLPLDSMAAPWETGDTFPDVVAIAPDVRATSSPGLRDGSPDVTTADIGANTPDATKGCPDVQASLPDAKAKSPPTMVDSLLAVTLAGNLGLTFLRGSQTQSHPDLGTEGCWDQLSAPRTFTLLDPKASLLTMAFLNGALDGVILGDYLSRTPEPRPSLSHLLSQYYGAGVARDPGFRSNFRRQNGAALTSASILAQQVWGTLVLLQRLEPVHLQLQCMSQEQLAQVAANATKEFTEAFLGCPAIHPRCRWGAAPYRGRPKLLQLPLGFLYVHHTYVPAPPCTDFTRCAANMRSMQRYHQDTQGWGDIGYSFVVGSDGYVYEGRGWHWVGAHTLGHNSRGFGVAIVGNYTAALPTEAALRTVRDTLPSCAVRAGLLRPDYALLGHRQLVRTDCPGDALFDLLRTWPHFTATVKPRPARSVSKRSRREPPPRTLPATDLQ
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
62.0kDa
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description

The recombinant Human PGLYRP2 protein production in Yeast cells involves the integration of a DNA fragment encoding the Human PGLYRP2 protein (22-576aa) into a plasmid vector, which is then introduced into Yeast cells. Following screening, positive cells are cultured and induced to synthesize the PGLYRP2 protein. A N-terminal 6xHis tag is attached to the protein. Cell lysis is carried out to harvest the recombinant Human PGLYRP2 protein, which is purified through affinity purification and then analyzed using SDS-PAGE and subsequent staining of the gel with Coomassie Brilliant Blue. The purity of the resulting recombinant Human PGLYRP2 protein reaches over 90%.

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Target Background

Function
May play a scavenger role by digesting biologically active peptidoglycan (PGN) into biologically inactive fragments. Has no direct bacteriolytic activity.
Gene References into Functions
  1. This study demonistrated that PGLYRP2 single-nucleotide polymorphisms is risk of Parkinson's disease. PMID: 24838182
  2. Various types of human blood cells were tested for expression of the Tag7/PGRP-SA and TagL/PGRP-L proteins, which belong to the family of proteins possessing the lysozyme-like peptidoglycan recognition protein (PGRP) domain PMID: 12669421
  3. Serum N-acetylmuramoyl-l-alanine amidaseand liver PGLYRP2 are the same protein encoded by the pglyrp2 gene. PMID: 16054449
  4. PGLYRP2 may function in the skin and the eyes as an inducible scavenger of proinflammatory peptidoglycan. PMID: 16239516
  5. pglyrp2 expression in skin and liver is important for systemic and local innate immune responses to bacterial infections PMID: 16714290

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Subcellular Location
Secreted. Membrane.
Protein Families
N-acetylmuramoyl-L-alanine amidase 2 family
Tissue Specificity
Strongly expressed in liver and fetal liver, and secreted into serum. Expressed to a much lesser extent in transverse colon, lymph nodes, heart, thymus, pancreas, descending colon, stomach and testis. Isoform 2 is not detected in the liver or serum.
Database Links

HGNC: 30013

OMIM: 608199

KEGG: hsa:114770

STRING: 9606.ENSP00000345968

UniGene: Hs.282244

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