Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

PSG1

Uniprot No.

P11464

Research Area

Tags & Cell Markers

Alternative Names

(PS-beta-G-1)(PSBG-1)(Pregnancy-specific glycoprotein 1)(CD66 antigen-like family member F)(Fetal liver non-specific cross-reactive antigen 1/2)(FL-NCA-1/2)(PSG95)(Pregnancy-specific beta-1 glycoprotein C/D)(PS-beta-C/D)(CD antigen CD66f)

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

35-419aa

Target Protein Sequence

QVTIEAEPTKVSEGKDVLLLVHNLPQNLTGYIWYKGQMRDLYHYITSYVVDGEIIIYGPAYSGRETAYSNASLLIQNVTREDAGSYTLHIIKGDDGTRGVTGRFTFTLHLETPKPSISSSNLNPRETMEAVSLTCDPETPDASYLWWMNGQSLPMTHSLKLSETNRTLFLLGVTKYTAGPYECEIRNPVSASRSDPVTLNLLPKLPKPYITINNLNPRENKDVLNFTCEPKSENYTYIWWLNGQSLPVSPRVKRPIENRILILPSVTRNETGPYQCEIRDRYGGIRSDPVTLNVLYGPDLPRIYPSFTYYRSGEVLYLSCSADSNPPAQYSWTINEKFQLPGQKLFIRHITTKHSGLYVCSVRNSATGKESSKSMTVEVSDWTVP
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

49.5 kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The production of recombinant Human PSG1 protein involves manipulating the expression of the desired gene in e.coli cells. The sequence (35-419aa) of foreign DNA is merged with an expression vector and subsequently introduced into e.coli cells. Positive cells are chosen and cultivated to induce the expression of the desired protein. A N-terminal 6xHis tag is fused to the protein. The resulting recombinant Human PSG1 protein undergoes purification through affinity purification, with a purity exceeding 85%, as confirmed by SDS-PAGE.

Target Background

Gene References into Functions

Inhibiting PSG1 can effectively reduce chemoresistance via a transforming growth factor-beta (TGF-beta)-related pathway in ER-negative breast cancer cells. PMID: 27653744
PSG stimulated IDO activity under the conditions of induction of the monocytes by interferon-gamma. PMID: 27595833
Under conditions of directed induction of phenotypic changes in CD4(+) lymphocytes to regulatory T cells (Treg), pregnancy-specific at high concentrations (10 and 100 mug/mL) increased FOXP3 expression. The evaluation of the spontaneous expression level of FOXP3 mRNA showed that pregnancy-specific beta1-glycoprotein (1 and 100 mug/mL) stimulated the expression of this factor in mononuclear cells and isolated CD4(+) lym... PMID: 27817017
results identify a non-placental site of PSG expression in the gut and associated tumors, with implications for determining whether PSGs have a role in tumor progression, and utility as tumor biomarkers. PMID: 26926266
the presence of GAGs on the surface of endothelial cells was required for the ability of PSG1 to induce tube formation PMID: 21193412
The aspartic acid at position 95 is not required for PSG1 activity but the amino acids implicated in the formation of a salt bridge within the N-domain are essential. PMID: 20335639
first trimester maternal serum marker of small-for-gestational age and preterm delivery PMID: 19911417
Human PSG1a induces alternative activation in human & mouse monocytes & suppresses the accessory-cell-dependent T-cell proliferation. PSG1a contributes to immunomodulation during pregnancy, with opposite effects on maternal innate & adaptative systems. PMID: 12223519
The differential expression of HLA-E and PSG1 in the sperm of fertile and infertile men is discussed. PMID: 18987160

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Subcellular Location

Secreted.

Protein Families

Immunoglobulin superfamily, CEA family

Database Links

HGNC: 9514

OMIM: 176390

KEGG: hsa:5669

STRING: 9606.ENSP00000244296

UniGene: Hs.709192

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Code	CSB-EP018849HU
Abbreviation	Recombinant Human PSG1 protein
MSDS	MSDS Datasheet
Size	$256
	Order now
Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Pregnancy-specific beta-1-glycoprotein 1 (PSG1)

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