Recombinant Human Prepronociceptin (PNOC)

Code CSB-YP018278HU
MSDS
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Source Yeast
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Code CSB-EP018278HU
MSDS
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Source E.coli
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Code CSB-EP018278HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP018278HU
MSDS
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Source Baculovirus
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Code CSB-MP018278HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PNOC
Uniprot No.
Alternative Names
N/OFQ; N23K; N23K/N27K; Nociceptin, included; nocistatin; Npnc1; OFQ; OFQ/N; Orphanin FQ; Orphanin FQ2; Pnoc; PNOC_HUMAN; ppN/OFQ; PPNOC; pre-pro-N/OFQ; prepronociceptin; pronociceptin; Propronociceptin
Species
Homo sapiens (Human)
Expression Region
98-127
Target Protein Sequence
MPR VRSLFQEQEE PEPGMEEAGE MEQKQLQ
Protein Length
Cytoplasmic domain
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Ligand of the opioid receptor-like receptor OPRL1. It may act as a transmitter in the brain by modulating nociceptive and locomotor behavior. May be involved in neuronal differentiation and development.; Blocks nociceptin action in pain transmission by inhibiting nociceptin-induced hyperalgesia and allodynia.; Has potent analgesic activity.
Gene References into Functions
  1. found differences in Neonatal Abstinence Syndrome outcomes depending on PNOC and COMT SNP genotype. PMID: 27983768
  2. The presence of the PNOC rs732636 A allele was associated with the worse Neonatal abstinence syndrome outcomes in infants. PMID: 26791055
  3. Data suggest that nociceptin plays a crucial role in progress of meiosis during spermatogenesis. [REVIEW] PMID: 25677772
  4. Data suggest that both nociceptin and nocistatin inhibit myometrial contractions and may play roles in perception of pain during delivery. [REVIEW] PMID: 25677774
  5. REVIEW: intracellular localization and physiological role of nociceptin and nocistatin PMID: 23454174
  6. Strong expression of PNOC was seen in neoplastic neurons of 7/7 brainstem gangliogliomas, but was significantly weaker in non-brainstem GGs. PMID: 22706982
  7. The presence of Orphanin, Oxytocin, ANP, eNOS and iNOS proteins was identified in the human umbilical cord.[Orphanin] PMID: 21744319
  8. A high expression of nociceptin in adenomyosis may be one of the casual factors of dysmenorrhea. The severity of dysmenorrhea is positively correlated with the expression of nociceptin in eutopic endometrial tissue. PMID: 21600132
  9. chronic ischemic conditions of atherosclerotic origin are associated with significantly lower plasma nociceptin levels PMID: 21310194
  10. PNOC expression level was 2.99 times higher in vitiligo involved skin when compared to healthy control skin. PMID: 20888736
  11. Results showed that vigorous and prolonged hyperventilation changes plasma nociceptin/orphanin FQ levels due to the direct effects of hypocapnic alkalosis or to different sympatho-adrenergic system responses. PMID: 20100531
  12. Peripheral blood levels are elevated in Wilson disease PMID: 12812047
  13. results show that nociceptin/NociR is present and functional in human neutrophils, and the results identify a novel dialogue pathway between neural and immune tissues PMID: 12950177
  14. The presence of two different forms of mature nocistatin (NST-17 and NST-30) and a possible N-terminal methionine cleaved NST-29 have been confirmed by both radioimmunoassay and mass spectrometry. PMID: 16043263
  15. Two SNPs in PNOC showed marginal association with alcoholism and one with illicit drug dependence. PMID: 17910740
  16. Peripheral blood mononuclear cells transcribe pre-pro-nociception/orphaninFQ. PMID: 18292431
  17. Unstable angina is associated with a significant increase in circulating nociceptin/orphanin FQ levels unrelated to intervening transient myocardial ischemia or hemodynamic changes. PMID: 19560501

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Subcellular Location
Secreted.
Protein Families
Opioid neuropeptide precursor family
Tissue Specificity
Predominantly expressed in the brain and spinal cord. Also expressed and secreted by peripheral blood neutrophils following degranulation.
Database Links

HGNC: 9163

OMIM: 601459

KEGG: hsa:5368

STRING: 9606.ENSP00000301908

UniGene: Hs.88218

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