Recombinant Human RNA demethylase ALKBH5(ALKBH5)

Code CSB-YP744062HU
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Source Yeast
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Code CSB-EP744062HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP744062HU
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Source Baculovirus
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Code CSB-MP744062HU
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names ALKBH5
Uniprot No. Q6P6C2
Alternative Names ALKBH5; ABH5; OFOXD1RNA demethylase ALKBH5; EC; Alkylated DNA repair protein alkB homolog 5; Alpha-ketoglutarate-dependent dioxygenase alkB homolog 5
Species Homo sapiens (Human)
Expression Region 2-394
Protein Length Full Length of Mature Protein
Tag Info The following tags are available.
N-terminal His-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Dioxygenase that demethylates RNA by oxidative demethylation: specifically demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes. Can also demethylate N(6)-methyladenosine in single-stranded DNA (in vitro). Requires molecular oxygen, alpha-ketoglutarate and iron. Demethylation of m6A mRNA affects mRNA processing and export. Required for the late meiotic and haploid phases of spermatogenesis by mediating m6A demethylation in spermatocytes and round spermatids: m6A demethylation of target transcripts is required for correct splicing and the production of longer 3'-UTR mRNAs in male germ cells.
Gene References into Functions
  1. Overexpression of ALKBH5 in MIA-PaCa-2 and BxPC-3 cells, demonstrats that ALKBH5 could inhibit cell migration and invasion and ALKBH5 inhibits cell motility by demethylating and increasing expression of KCNK15- AS1. PMID: 30032148
  2. N(6)-Methyladenosine itself serves as a 'conformational marker', which induces different conformational outcomes in RNAs depending on sequence context. This critically impacts its interactions with several m6A-recognising proteins, including FTO and ALKBH5. PMID: 27156733
  3. ALKBH5 knockdown in breast cancer cells significantly decreased metastasis from breast to lungs in immunodeficient mice PMID: 27590511
  4. ALKBH5-FOXM1 pathway is critical for glioblastoma proliferation and tumorigenesis.ALKBH5 expression increases in glioblastoma stem-like cells and predicts poor survival in glioblastoma patients. PMID: 28344040
  5. HIF-dependent ALKBH5 expression mediates enrichment of BCSCs in the hypoxic tumor microenvironment PMID: 27001847
  6. The ALKBH5 gene may play a role in conferring risk of MDD in the Chinese population. PMID: 26047305
  7. Structures of human ALKBH5 demethylase reveal a unique binding mode for specific single-stranded N6-methyladenosine RNA demethylation. PMID: 24778178
  8. Modelling substrate into the active site of ALKBH5 reveals conserved residues important for recognition and demethylation mechanisms. PMID: 24489119
  9. findings provide a structural basis for understanding the substrate recognition specificity of Alkbh5 and offer a foundation for selective drug design against AlkB members PMID: 24616105
  10. ALKBH5 is a RNA demethylase and its action strongly suggests that the reversible methyladenosine modification has fundamental and broad functions in mammalian cells. PMID: 23177736
  11. ALKBH5 may have a role in the regulation of cellular responses to hypoxia as a class of HIF-transcriptional target gene PMID: 21264265

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Subcellular Location Nucleus speckle.
Protein Families AlkB family
Tissue Specificity Widely expressed, with highest expression in lung, followed by testis, pancreas, spleen and ovary.
Database Links

HGNC: 25996

OMIM: 613303

KEGG: hsa:54890

STRING: 9606.ENSP00000382091

UniGene: Hs.744130

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