Recombinant Human Protein Hook homolog 1 (HOOK1)

Code CSB-YP891526HU
MSDS
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Source Yeast
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Code CSB-EP891526HU
MSDS
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Source E.coli
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Code CSB-EP891526HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP891526HU
MSDS
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Source Baculovirus
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Code CSB-MP891526HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Uniprot No.
Alternative Names
A930033L17Rik; Abnormal spermatozoon head shape; azh; h-hook1; hHK1; HK1; HOOK 1; Hook homolog 1 (Drosophila); Hook1; HOOK1_HUMAN; MGC10642; OTTHUMP00000010548; OTTMUSP00000008480; Protein Hook homolog 1; RP23-80B16.4
Species
Homo sapiens (Human)
Expression Region
1-728
Target Protein Sequence
MEETQPPPQP KLPLCDSLMI WLQTFNTASP CQDVKQLTSG VAMAQVLHQI DAAWFNESWL SRIKEDVGDN WRIKASNVKK VLQGIMSYYH EFLGQQISEA LIPDLNQITE CSDPVELGRL LQLILGCAIN CEKKQEHIQN IMTLEESVQH VVMTAIQELM SKEILSSPPN DAVGELEQQL KRALEELQEA LAEKEELRQR CEELDMQVTT LQDEKNSLVS ENEMMNEKLD QLDGSFDDPN TVVAKKYFHA QLQLEQLQEE NFRLEAAKDD YRVHCEELEK QLIEFQHRND ELTSLAEETR ALKDEIDVLR ATSDKANKLE STVEIYRQKL QDLNDLRKQV KTLQETNMMY MHNTVSLEEE LKKANAARTQ LETYKRQVQD LHVKLSSESK RADTLAFEMK RLEEKHEALL KEKERLIEQR DTLKETNEEL RCSQVQQDHL NQTDASATKS YENLAAEIMP VEYREVFIRL QHENKMLRLQ QEGSENERIE ELQEQLEQKH RKMNELETEQ RLSKERIREL QQQIEDLQKS LQEQGSKSEG ESSSKLKQKL EAHMEKLTEV HEELQKKQEL IEDLQPDINQ NVQKINELEA ALQKKDEDMK AMEERYKMYL EKARNVIKTL DPKLNPASAE IMLLRKQLAE KERRIEILES ECKVAKFRDY EEKLIVSAWY NKSLAFQKLG MESRLVSGGG ACSDTGACTP ARSFLAQQRH ITNTRRNLSV KVPATTSD
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex). FHF complex promotes the distribution of AP-4 complex to the perinuclear area of the cell. Required for spermatid differentiation. Probably involved in the positioning of the microtubules of the manchette and the flagellum in relation to the membrane skeleton.
Gene References into Functions
  1. Hook1 knockdown promoted epithelial-mesenchymal transition and attenuated the sensitivity of hepatocellular carcinoma cells to doxorubicin. In summary, our results indicate that downregulation of Hook1 plays a pivotal role in hepatocellular carcinoma progression via epithelial-mesenchymal transition. Hook1 may be used as a novel marker and therapeutic molecular target in hepatocellular carcinoma PMID: 28718370
  2. Single-molecule motility assays using total internal reflection fluorescence microscopy indicate that both Hook1 and Hook3 effectively activate cytoplasmic dynein, inducing longer run lengths and higher velocities than the previously characterized dynein activator bicaudal D2 (BICD2). PMID: 27365401
  3. SHP2 positively regulates TGFbeta1-induced epithelial-mesenchymal transition modulated by its novel interacting protein Hook1. PMID: 25331952
  4. Hook1, a microtubule- and cargo-tethering protein, recognized the cytoplasmic tail of CD147 to help sort it and CD98 into Rab22a-dependent tubules associated with recycling. PMID: 23589492
  5. novel interactions between the microtubule-binding Hook1 and the large family of Rab GTPases also suggest a link between CLN3 function, microtubule cytoskeleton and endocytic membrane trafficking PMID: 15471887
  6. These data suggest that the FTS/Hook/FHIP complex functions to promote vesicle trafficking and/or fusion via the homotypic vacuolar protein sorting complex. PMID: 18799622

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Subcellular Location
Cytoplasm. Cytoplasm, cytoskeleton.
Protein Families
Hook family
Database Links

HGNC: 19884

OMIM: 607820

KEGG: hsa:51361

STRING: 9606.ENSP00000360252

UniGene: Hs.378836

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