Recombinant Human Protein Wnt-9b(WNT9B)

Code CSB-YP026146HU
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Source Yeast
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Code CSB-EP026146HU
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Source E.coli
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Code CSB-EP026146HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP026146HU
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Source Baculovirus
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Code CSB-MP026146HU
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names WNT9B
Uniprot No. O14905
Alternative Names WNT9B; WNT14B; WNT15; UNQ6973/PRO21956; Protein Wnt-9b; Protein Wnt-14b; Protein Wnt-15
Species Homo sapiens (Human)
Expression Region 23-357
Target Protein Sequence SYFGLTGR EVLTPFPGLG TAAAPAQGGA HLKQCDLLKL SRRQKQLCRR EPGLAETLRD AAHLGLLECQ FQFRHERWNC SLEGRMGLLK RGFKETAFLY AVSSAALTHT LARACSAGRM ERCTCDDSPG LESRQAWQWG VCGDNLKYST KFLSNFLGSK RGNKDLRARA DAHNTHVGIK AVKSGLRTTC KCHGVSGSCA VRTCWKQLSP FRETGQVLKL RYDSAVKVSS ATNEALGRLE LWAPARQGSL TKGLAPRSGD LVYMEDSPSF CRPSKYSPGT AGRVCSREAS CSSLCCGRGY DTQSRLVAFS CHCQVQWCCY VECQQCVQEE LVYTCKH
Protein Length Full Length of Mature Protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Data

Function Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters (By similarity).
Gene References into Functions
  1. Our study further supports the involvement of WNT9B as a cleft susceptibility gene in Brazilian families experiencing NSCL+/-P. Although additional studies are still necessary to unveil the exact mechanism by which WNT genes would contribute to NSCL+/-P, allelic polymorphisms in these genes and their interactions may partly explain the variance of individual susceptibility to NSCL+/-P. PMID: 24437584
  2. Multi-layered mutation in hedgehog-related genes in Gorlin syndrome may affect the phenotype PMID: 28915250
  3. This association study successfully identified two susceptibility Single Nucleotide Polymorphisms, (WNT9B and PBX1) associated with Mayer-Rokitansky-Kuster-Hauser syndrome risk, both separately and interactively. PMID: 26075712
  4. results indicate that mutations in the coding sequence of WNT9B are not responsible for Mullerian duct abnormalities in the Chinese population PMID: 24581601
  5. Two novel mutations (a missense mutation in exon 1, and one in the 3-UTR) may be pathogenic variants in Mayer-Rokitansky-Kuster-Hauser syndrome patients and warrant further functional study. PMID: 24268733
  6. The signals from the stromal fibroblasts cooperate with Wnt9b to promote differentiation of the progenitor cells. PMID: 23974041

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Subcellular Location Secreted, extracellular space, extracellular matrix
Protein Families Wnt family
Tissue Specificity Moderately expressed in fetal kidney and adult kidney. Also found in brain.
Database Links

HGNC: 12779

OMIM: 602864

KEGG: hsa:7484

STRING: 9606.ENSP00000290015

UniGene: Hs.326420

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