Purity
Greater than 90% as determined by SDS-PAGE.
Research Area
Cell Biology
Alternative Names
SFTPB; SFTP3; Pulmonary surfactant-associated protein B; SP-B; 18 kDa pulmonary-surfactant protein; 6 kDa protein; Pulmonary surfactant-associated proteolipid SPL(Phe
Species
Homo sapiens (Human)
Expression Region
201-279aa
Target Protein Sequence
FPIPLPYCWLCRALIKRIQAMIPKGALAVAVAQVCRVVPLVAGGICQCLAERYSVILLDTLLGRMLPQLVCRLVLRCSM
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Full Length of Mature Protein
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
The recombinant Human SFTPB protein is encoded by the gene of SFTPB (201-279aa). The gene of SFTPB was cloned in a system (Yeast) that supported the expression of SFTPB. Modification of SFTPB by recombinant DNA technology could lead to the expression of the target protein. The purity is 90% determined by SDS-PAGE.
SFTPB is a protein coding gene that encodes Pulmonary surfactant-associated protein B. According to some studies, SFTPB may have the following features.
The genetic relationship between lung surfactant protein genes SFTPA1, SFTPA2, SFTPB, SFTPC and SFTPD and cystic fibrosis. Mutations in the thyroid transcription factor gene NKX2-1 resulted in decreased expression of SFTPB and SFTPC. The pattern in the CA repeat-rich region of surfactant protein B (SFTPB) intron 4 has different effects on mRNA splicing. Analyze 40 cases of sporadic or familial neonatal and pediatric severe unexplained respiratory distress. The relationship with SFTPB. SFTPB gene lineage evolutionary event. Alu recombination-mediated deletion (ARMD), exonization and alternative splicing events. The upregulation of Sftpb induced by estrogen requires the transcriptional control of the neuroglutenin receptor ErbB4 in mouse lung type II epithelial cells.