Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

SFTPB

Uniprot No.

P07988

Research Area

Cell Biology

Alternative Names

SFTPB; SFTP3; Pulmonary surfactant-associated protein B; SP-B; 18 kDa pulmonary-surfactant protein; 6 kDa protein; Pulmonary surfactant-associated proteolipid SPL(Phe

Species

Homo sapiens (Human)

Source

Yeast

Expression Region

201-279aa

Target Protein Sequence

FPIPLPYCWLCRALIKRIQAMIPKGALAVAVAQVCRVVPLVAGGICQCLAERYSVILLDTLLGRMLPQLVCRLVLRCSM
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

8.7kDa

Protein Length

Full Length of Mature Protein

Tag Info

Tag-Free

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The recombinant Human SFTPB protein is encoded by the gene of SFTPB (201-279aa). The gene of SFTPB was cloned in a system (Yeast) that supported the expression of SFTPB. Modification of SFTPB by recombinant DNA technology could lead to the expression of the target protein. The purity is 90% determined by SDS-PAGE.

SFTPB is a protein coding gene that encodes Pulmonary surfactant-associated protein B. According to some studies, SFTPB may have the following features.
The genetic relationship between lung surfactant protein genes SFTPA1, SFTPA2, SFTPB, SFTPC and SFTPD and cystic fibrosis. Mutations in the thyroid transcription factor gene NKX2-1 resulted in decreased expression of SFTPB and SFTPC. The pattern in the CA repeat-rich region of surfactant protein B (SFTPB) intron 4 has different effects on mRNA splicing. Analyze 40 cases of sporadic or familial neonatal and pediatric severe unexplained respiratory distress. The relationship with SFTPB. SFTPB gene lineage evolutionary event. Alu recombination-mediated deletion (ARMD), exonization and alternative splicing events. The upregulation of Sftpb induced by estrogen requires the transcriptional control of the neuroglutenin receptor ErbB4 in mouse lung type II epithelial cells.

Target Background

Function

Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter.

Gene References into Functions

rs7316 polymorphism associated with respiratory distress syndrome in Iranian premature newborns PMID: 28738720
TGF beta markedly decreased expression of SP-A, SP-B, SP-C, fatty acid synthase, and the phospholipid transporter ABCA3. However, TGF beta increased protein levels of SP-D with little change in mRNA levels. PMID: 29621540
Surfactant protein B suppresses lung cancer progression by inhibiting secretory phospholipase A2 activity and arachidonic acid production. PMID: 28743125
Data show that the thermal transitions observed in N-terminal propeptide of proSP-B (SP-BN) may correspond to the unfolding of certain protein domains or subdomains and/or to oligomers dissociation whereas the overall protein shows resistance to temperature-induced unfolding up to ~ 86 degrees C. PMID: 27380171
SP-A (+186A/G) and SP-B (1580C/T) polymorphisms are strongly associated with the risk of respiratory distress syndrome (RDS) in preterm infants. Notably, reduced serum SP-A levels were correlated with a high risk of RDS and may serve as novel biomarkers for RDS detection and monitoring. PMID: 28011976
Differences were found regarding SPA and pro-SPB expression in the vast majority of subjects: in some lungs, SPA was more expressed whereas in others pro-SPB showed an higher degree of immunoreactivity. The expression of both surfactant proteins was not strictly correlated with gestational age. PMID: 27734990
There is no significant association between the gene polymorphism of the R236C site in exon 7 of SP-B gene and the incidence ofneonatal respiratory distress syndrome (NRDS) in Han populations in that region. PMID: 27655533
Rare mutations in surfactant-associated genes contribute to neonatal respiratory distress syndrome. The frequency of mutations in these genes in the Chinese population is unknown. We resequenced all exons of the surfactant protein-B (SFTPB) and we did not find any rare mutations in SFTPB PMID: 26547207
mice with SP-B-C allele are more susceptible to S. aureus pneumonia than mice with SP-B-T allele, and that CMC2.24 attenuates lung injury thus reducing mortality. PMID: 26863117
In term newborns with pneumonia, SP-B increases with respect to total phospholipids, and disaturated-phosphatidylcholine and is turned over at a faster rate. PMID: 26107393
SP-B -18C/A and 1580C/T polymorphisms are associated with bronchopulmonary dysplasia PMID: 26045806
These results demonstrate for the first time that human SP-B C allele is more susceptible to bacterial pneumonia than SP-B T allele in vivo. PMID: 26620227
Significant differences in frequency of occurrence of unfavorable genotypes CC rs1965708, AA rs1059046 of SFTPA2 gene and CC rs1130866 of SFTPB gene in influenza patients in comparison with individuals of the control group were not detected. PMID: 26950992
Investigated the relationship between SP-A2 and SP-B gene polymorphisms and respiratory distress syndrome in preterm neonates. PMID: 26061924
Given these fact SP-B deficiency may be considered as a further field for research on regenerative therapy i. e. stem cell administration in models of postnatal SP-B deficiency. PMID: 25565191
Results support a critical role of SP-B for promoting pulmonary surface film formation. PMID: 25360829
SFTPB variants are associated with chronic obstructive pulmonary disease susceptibility and lung function in the Chinese Han population. PMID: 25299874
Surfactant protein B gene polymorphism is associated with severe influenza. PMID: 24337193
data support the hypothesis that proSP-B glycosylation due to Ile131Thr variation may have a causal role in genetic susceptibility to acute respiratory distress PMID: 24002332
It was concluded that the polymorphisms of SP-B intron 4 and C/A-18 could be associated with BPD in Chinese Han infants, and the del allele of intron 4 and A allele of C/A-18 might be used as markers of susceptibility in the disease. PMID: 23771654
there was no evidence for any association between common polymorphisms in the SP-B and SP-D genes and COPD risk (all p>0.05). PMID: 24093802
Glucocorticoids mediated surfactant protein B production, maturation, and release is amplified by caffeine. PMID: 24163141
macrophages participate in the repression of SFTPB expression by LPS, and that macrophage-released cytokines (including TNF) regulate the transcription factor CEBPB PMID: 23590297
D2S388-5 microsatellite polymorphism of surfactant protein B may be associated with susceptibility to COPD in Xinjiang Kazakhs. PMID: 23088317
elevated expression in nasal mucosa in chronic rhinosinusitis with polyps PMID: 23406594
Mutations in exon 4 of the surfactant protein B gene demonstrate an association between homozygous mutations with C/C genotype in SP-B gene and neonatal respiratory distress. PMID: 23330012
caffeine is able to induce the expression of SP-transcription factors and affects the signaling pathways of glucocorticoids, amplifying their effects PMID: 23272120
The results indicate that tryptophan oxidation causes substantial disruptions in helical structure and lipid interactions. PMID: 21128671
review focuses on published association studies of surfactant proteins A and D genetic polymorphisms with respiratory, and non-respiratory diseases in adults, children, and newborns [review] PMID: 22201752
The present study demonstrated that the AG genotype of AG 9306 polymorphism was a protective factor against the development of respiratory distress syndrome, other polymorphisms studied (G/C at 8714, C/T at 1580, A/C at -18) were not associated with RDS. PMID: 21180884
In patients undergoing major vascular abdominal surgery, SP-B values increase progressively during mechanical ventilation stages compared to advanced glycation end-products (RAGE) kinetics. PMID: 21736957
SP-B 1580C/T polymorphism contributes to the etiology of respiratory distress syndrome. PMID: 22289747
Circulating levels of SP-B increase with greater smoking burden and independently associate with abdominal abdominal aortic plaque among current smokers. PMID: 21817103
Genetic variations in the gene coding for surfactant protein B are associated with more severe lung injury as indicated by the association of specific single nucleotide polymorphism genotypes and haplotypes PMID: 21283003
Surfactant protein B and RAGE increases in the plasma during cardiopulmonary bypass PMID: 20650982
Surfactant protein B polymorphisms have a role in pulmonary function and COPD PMID: 20693256
Disaturated-phosphatidylcholine and surfactant protein-B have a different turnover both in healthy and diseased lungs. PMID: 21429235
Absence of this protein resulted in Congenital pulmonary alveolar proteinosis in two newborn children PMID: 20005435
Surfactant protein-B 121ins2 heterozygosity is associated with reduced lung function and increased risk for COPD among smokers PMID: 19833825
review of lipid-protein interactions of hydrophobic proteins SP-B and SP-C in lung surfactant assembly and dynamics PMID: 11699574
Data suggest that SMAD3 interactions with the positive regulators NKX2.1 and HNF-3 underlie the molecular basis for TGF-beta-induced repression of surfactant protein B gene transcription. PMID: 12161428
role of Differences in N-linked glycosylation between human surfactant protein-B variants of the C or T allele at the single-nucleotide polymorphism at position 1580 in disease PMID: 12356334
suggest that polymorphisms in intron 4 of the surfactant protein B gene independently modify the course of neonatal respiratory distress syndrome PMID: 12424586
An association between SP-B polymorphism and RDS was found in premature, first-born twins. In particular, the threonine allele was associated with an increased risk of RDS. PMID: 12483294
dimeric SP-B(1-25) is more efficient in restoring lung function in neonatal RDS and ARDS than monomeric SP-B(1-25) surfactant. PMID: 12490037
surfactant protein B expression is regulated by an enhancer region which binds to thyroid transcription factor-1, retinoic acid receptor, signal transducers and activators of transcription 3, nuclear receptor coactivators (SRC-1, ACTR, TIF2, and CBP/p300 PMID: 12573987
Mutataions and Polymorphisms in SFTPB is associated with severe unexplained respiratory distress PMID: 12784301
SP-B expression is regulated by PPARgamma in the lung PMID: 12829715
Reduced SP-B expression due to elevated nitric oxide levels can contribute to lung injury. PMID: 12896877
In human lungs, mature SP-B is involved in the structural organization of lamellar bodies and tubular myelin by the formation of core particles. PMID: 12972403

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Involvement in disease

Pulmonary surfactant metabolism dysfunction 1 (SMDP1); Respiratory distress syndrome in premature infants (RDS)

Subcellular Location

Secreted, extracellular space, surface film.

Database Links

HGNC: 10801

OMIM: 178640

KEGG: hsa:6439

STRING: 9606.ENSP00000377409

UniGene: Hs.512690

Code	CSB-YP021173HU
Abbreviation	Recombinant Human SFTPB protein
MSDS	MSDS Datasheet
Size	$472
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Recombinant Human Pulmonary surfactant-associated protein B (SFTPB)

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