Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

PPP2R2B

Uniprot No.

Q00005

Research Area

Neuroscience

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

1-443aa

Target Protein Sequence

MEEDIDTRKINNSFLRDHSYATEADIISTVEFNHTGELLATGDKGGRVVIFQREQESKNQVHRRGEYNVYSTFQSHEPEFDYLKSLEIEEKINKIRWLPQQNAAYFLLSTNDKTVKLWKVSERDKRPEGYNLKDEEGRLRDPATITTLRVPVLRPMDLMVEATPRRVFANAHTYHINSISVNSDYETYMSADDLRINLWNFEITNQSFNIVDIKPANMEELTEVITAAEFHPHHCNTFVYSSSKGTIRLCDMRASALCDRHTKFFEEPEDPSNRSFFSEIISSISDVKFSHSGRYIMTRDYLTVKVWDLNMENRPIETYQVHDYLRSKLCSLYENDCIFDKFECVWNGSDSVIMTGSYNNFFRMFDRNTKRDVTLEASRENSKPRAILKPRKVCVGGKRRKDEISVDSLDFSKKILHTAWHPSENIIAVAATNNLYIFQDKVN
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

56.7 kDa

Protein Length

Full Length

Tag Info

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The expression of recombinant human serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B beta isoform (PPP2R2B) is achieved in E.coli, where a suitable expression vector is utilized to encode the full-length human PPP2R2B protein (1-443aa). This expression construct incorporates an N-terminal 10xHis-tag and a C-terminal Myc-tag to facilitate the purification and detection of the recombinant PPP2R2B protein. The purity of the resulting protein is determined to be up to 85% through SDS-PAGE analysis. On the gel, the purified recombinant human PPP2R2B protein appears as a band with an approximate molecular weight of 57 kDa. This recombinant human PPP2R2B protein enables researchers to investigate its role and impact on various neurobiological phenomena.

Target Background

Function

The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. Within the PP2A holoenzyme complex, isoform 2 is required to promote proapoptotic activity. Isoform 2 regulates neuronal survival through the mitochondrial fission and fusion balance.

Gene References into Functions

Dephosphorylation and total levels of beta-catenin were dependent on PR55beta in an adenoid cystic carcinoma tumor cell line. PMID: 29275362
New pathogenic threshold of PPP2R2B CAG-43 to be pathogenic for SCA12 diagnosis. PMID: 27864267
CAG triplet repeat expansion has been found in untranslated region of SCA12 gene in Indian spinocerebellar ataxia 12 patients. PMID: 27350687
Thus, Fcp1 coordinates Cdk1 and Gwl inactivation to derepress PP2A-B55, generating a dephosphorylation switch that drives mitosis progression. PMID: 26653855
investigated CAG copies within PPP2R2B in 29 patients with spinocerebellar ataxia who are excluded from the most common subtypes; 3 patients carried 53, 46 and 54 CAG repeats respectively, while the other 26 cases harbored CAG repeats less than 30 PMID: 25634432
IER5 functions as a positive feedback regulator of HSF1 and that this process involves PP2A/B55 and HSF1 dephosphorylation. PMID: 25816751
Bcl-2 phosphorylation at Ser70 by selective tyrosine nitration of PP2A-B56delta stabilizes its antiapoptotic activity PMID: 25082878
Inactivation of protein-phosphatase 2A causing hyperphosphorylation of paraprotein targets in monoclonal gammopathy of undetermined significance, multiple myeloma and Waldenstrom's macroglobulinemia is due to an exchange of its regulatory subunits PMID: 24676687
We demonstrate that PP2A/B55 is required for Gwl dephosphorylation at the essential Cdk site Thr194. Ensa/ARPP19 dephosphorylation is mediated by the RNA PMID: 24391510
PP2A-B55beta promotes cyclin E1 overexpression by antagonizing its degradation and its inhibition could represent a therapeutic mechanism for abrogating cyclin E1 function in cancers PMID: 24509904
Bod1 regulates protein phosphatase 2A at mitotic kinetochores. PMID: 24157919
Differential autophagic neuronal cell death under stress is determined by the cytoplasmic and mitochondrial-specific PPP2R2B isoforms. PMID: 23381641
Single nucleotide polymorphisms in PPP2R2B is associated with drug response in breast cancer. PMID: 23034890
Data indicate that PPP2R2B gene product PP2A/Bbeta2 activates the mitochondrial fission enzyme dynamin-related protein 1 (Drp1) by dephosphorylating Ser656. PMID: 22583914
This study suggested that PPP2R2B CAG expansion mutation might lead increase induction of Spinocerebellar ataxia type 12. PMID: 21827912
defective production of PP2A Bbeta upon IL-2 deprivation results in apoptosis resistance and longer survival of autoreactive T cells, in a subset of SLE patients PMID: 21746932
oxidative stress induced by mitochondrial dysfunction causes elevated expression of ppp2r2b and plays a causal role in SCA12; and reduction of ROS is a potential therapeutic intervention for this neuropathy PMID: 21471219
results indicate that the A variant of the rs319217 SNP of the PPP2R2B gene is a marker of better prognosis in breast cancer PMID: 20669227
CAG repeat lengths in the PPP2R2B gene may be potential genetic markers for Alzheimer disease susceptibility in the Japanese population. PMID: 21029765
PPP2R2B, encoding the B55beta regulatory subunit of the PP2A complex, is epigenetically inactivated by DNA hypermethylation in colorectal cancer. PMID: 21075311
Autosomal dominant cerebellar ataxia mapping to 5q31-q33.1 has no CAG repeat expansion or other mutations of the PPP2R2B gene. PMID: 20937954
Quantitative methylation analysis identified ABCB1, FOXC1, PPP2R2B and PTEN as novel genes to be methylated in ductal carcinoma in situ. PMID: 20056007
The CAG repeat in SCA12 functions as a cis element to up-regulate PPP2R2B expression. PMID: 20533062
We investigated methylation patterns in the promoter regions of ABCB1, ATM, BRCA1, CDH3, CDKN2A, CXCR4, ESR1, FBXW7, FOXC1, GSTP1, IGF2, HMLH1, PPP2R2B, and PTEN75 in well-described pre-treatment samples from locally advanced breast cancer PMID: 20338046
Cells with ectopically expressed PPP2R2B were shown predisposed to autophagy and oxidative stress induced cell death. PMID: 20017961
a novel ATM-dependent mechanism is regulating association of B55 subunits with nuclear PP2A in response to IR PMID: 11723136
Cyclin G recruits PP2A in order to modulate the phosphorylation of Mdm2 and thereby to regulate both Mdm2 and p53. PMID: 11983168
Phosphorylation of bestrophin is regulated by PP2A. PMID: 12058047
SCA12 triplet expansion rate in Italian ataxia patients PMID: 12140678
Analysis of CTA/CTG and CAG polymorphism in a Polish control group. PMID: 14960773
phospholipase D suppresses protein phosphatase 2A and is involved in the mTOR survival pathway in the transformation of human cells PMID: 16109716
PP2A plays a positive rather than a negative role in the regulation of IKKbeta PMID: 16126728
analysis of a founder for SCA12 in the Indian population PMID: 16138911
We therefore propose that PP2A plays a crucial role in the maintenance of cell-cell adhesion through recruitment of IQGAP1 to the Rac-bound E-cadherin-catenins complex. PMID: 16245300
The tumor suppressor PP2A is functionally inactivated in blast crisis CML through the inhibitory activity of the BCR/ABL-regulated SET protein. PMID: 16286244
PP2A-mediated dephosphorylation of HSP27 and tau correlated with PP2A-induced preservation of endothelial cell cytoskeleton PMID: 16475161
Cyclin G2 may modulate the cell cycle and cellular division processes through modulation of PP2A and centrosomal associated activities. PMID: 17123511
The small t antigen (ST) of DNA tumor virus SV40 inhibits PP2A phosphatase activity through its N-terminal J domain. PMID: 17529992
results suggest that the PPP2R2B gene CAG repeat polymorphism may be functional and may, in part, play a role in conferring susceptibility to Alzheimer disease and essential tremor in Taiwan PMID: 18484086
Adenovirus E4orf4 protein downregulates MYC expression through interaction with the PP2A-B55 subunit PMID: 18653458
Results suggest that Parkinsonism (PD) and multiple system atrophy (MSA) are not associated with spinocerebellar ataxia 12 (SCA12) and it is not necessary to screen SCA12 in PD and MSA patients. PMID: 18973067
Clinical trial and genome-wide association study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator) PMID: 18519826

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Involvement in disease

Spinocerebellar ataxia 12 (SCA12)

Subcellular Location

[Isoform 1]: Cytoplasm. Cytoplasm, cytoskeleton. Membrane.; [Isoform 2]: Cytoplasm. Mitochondrion. Mitochondrion outer membrane.

Protein Families

Phosphatase 2A regulatory subunit B family

Tissue Specificity

Brain.

Database Links

HGNC: 9305

OMIM: 604325

KEGG: hsa:5521

STRING: 9606.ENSP00000336591

UniGene: Hs.627618

Code	CSB-EP018565HU
Abbreviation	Recombinant Human PPP2R2B protein
MSDS	MSDS Datasheet
Size	$306
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B beta isoform (PPP2R2B)

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