Recombinant Human Sodium-coupled neutral amino acid transporter 2 (SLC38A2), partial

Code CSB-YP847210HU
MSDS
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Source Yeast
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Code CSB-EP847210HU
MSDS
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Source E.coli
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Code CSB-EP847210HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP847210HU
MSDS
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Source Baculovirus
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Code CSB-MP847210HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
SLC38A2
Uniprot No.
Alternative Names
Amino acid transporter 2; Amino acid transporter A2; ATA2; KIAA1382; PRO1068; Protein 40-9-1; S38A2_HUMAN; SAT2; Slc38a2; SNAT2; Sodium-coupled neutral amino acid transporter 2; Solute carrier family 38 member 2; System A amino acid transporter; System A amino acid transporter 2; System A transporter 1; System N amino acid transporter 2
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Functions as a sodium-dependent amino acid transporter. Mediates the saturable, pH-sensitive and electrogenic cotransport of neutral amino acids and sodium ions with a stoichiometry of 1:1. May function in the transport of amino acids at the blood-brain barrier and in the supply of maternal nutrients to the fetus through the placenta.
Gene References into Functions
  1. there is a disulfide bond between Cys245 and Cys279 in SNAT2 which has no effect on cell surface trafficking, as well as transporter function PMID: 27355203
  2. In the absence of SLC1A5 there is a crucial role of SNAT1 in supplying glutamine for glutaminolysis with SNAT2 acting as a "backup" for glutamine transport. PMID: 27129276
  3. Propose regulation of placental SNAT2/LAT1 ubiquitination by mTORC1 and Nedd4-2. PMID: 26608079
  4. Decreased placental mTOR activity causes down-regulation of placental system A activity by shifting SNAT-2 trafficking towards proteasomal degradation, thereby contributing to decreased fetal amino acid availability and restricted fetal growth in IUGR. PMID: 26374858
  5. GADD34 promotes cell survival and adaptation to increased extracellular osmolarity by increasing the uptake of small neutral amino acids via the amino acid transporter SNAT2. PMID: 26041779
  6. Increased availability of unsaturated fatty acids can compromise the stress-induced induction/adaptation in SNAT2 expression. PMID: 25653282
  7. SLC38A1 and SLC38A2 transcript levels are altered in placental malaria with intervillositis. PMID: 23408887
  8. SNAT2 mRNA levels were significantly decreased in intrauterine growth-restricted placentas with reduced umbilical blood flows. PMID: 23728383
  9. Placental mRNA expression of system A transporter isoforms SLC38A1 and -2 was lower in teenagers than in adults which may underlie their susceptibility to delivering small-for-gestational-age infants. PMID: 22028413
  10. findings show methylation status of rRNA differentially influenced the mechanism of 80S complex formation on IRES elements from SNAT2)versus the hepatitis C virus mRNA PMID: 21930789
  11. Proteomic analysis of TAP-tag purified SNAT2 fusion proteins identified two novel SNAT2-interacting proteins that may potentially function in conjunction with SNAT2 transceptor to regulate signalling pathways influencing protein turnover and cell growth PMID: 21622135
  12. Lower placental system A activity in women who reported strenuous exercise and had a lower arm muscle area may reflect an adaptation in placental function which protects maternal resources in those with lower nutrient reserves. PMID: 20206993
  13. SNAT2 amino acid response element, along with a nearby conserved CAAT box, has enhancer activity and it confers regulated transcription to a heterologous promoter PMID: 14623874
  14. The synthesis of SNAT2 is required for the hypertonic stimulation of system A transport activity. PMID: 15581851
  15. Up-regulation of SNAT2 is essential for the rapid restoration of cell volume after hypertonic stress. PMID: 15922329
  16. Amino acid depletion was associated with an up-regulation of amino acid transport system A activity, largely mediated through an enhancement of SNAT2 expression at both the protein and mRNA level in BeWo cells. PMID: 16125834
  17. deficient in the amino acid response pathway exhibited little or no induction of SNAT2 mRNA PMID: 16445384
  18. The strong expression of Na(+)-coupled neutral amino acid transporter 2 in the somato-dendritic compartment and in non-neuronal elements that are integral parts of the blood-brain and brain-cerebrospinal fluid barrier. PMID: 16616430
  19. SNAT2 expression can be modulated by specific signaling pathways in response to different stresses PMID: 16621798
  20. Results suggest that cortisol may be involved in upregulation of system A (SNAT2)in the placenta to ensure sufficient amino acid supply to the developing fetus. PMID: 16621896
  21. Therefore, we can speculate that such anion-conducting pathways are general features of Na+-transporting systems. PMID: 17237199
  22. Despite increased ATF4 binding at the C/EBP-ATF composite site following activation of the unfolded protein response, system A transporter 2 (SNAT2) transcription activity is repressed PMID: 18697751
  23. Report SNAT2 activity in fetal T lymphocytes in intrauterine growth restriction and with tumor necrosis factor-alpha treatment. PMID: 18703994
  24. analysis of a conserved Na(+) binding site of the sodium-coupled neutral amino acid transporter 2 PMID: 19589777
  25. we have identified a novel regulatory pathway involving increased gene expression of the SNAT2 isoform mediated by a STAT-dependent pathway, which links IL-6 to increased activity of amino acid transporter system A PMID: 19741197

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Subcellular Location
Cell membrane; Multi-pass membrane protein.
Protein Families
Amino acid/polyamine transporter 2 family
Tissue Specificity
Ubiquitously expressed. Widely expressed in the central nervous system with higher concentrations in caudal regions. Expressed by glutamatergic and GABAergic neurons together with astrocytes and other non-neuronal cells in the cerebral cortex (at protein
Database Links

HGNC: 13448

OMIM: 605180

KEGG: hsa:54407

STRING: 9606.ENSP00000256689

UniGene: Hs.221847

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