Recombinant Human Sorting nexin-10 (SNX10)

Code CSB-YP896538HU
MSDS
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Source Yeast
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Code CSB-EP896538HU
MSDS
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Source E.coli
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Code CSB-EP896538HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP896538HU
MSDS
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Source Baculovirus
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Code CSB-MP896538HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
SNX10
Uniprot No.
Alternative Names
2410004M09Rik; MGC109202; MGC33054; OPTB8; SNX10; SNX10_HUMAN; Sorting nexin 10; Sorting nexin-10
Species
Homo sapiens (Human)
Expression Region
1-201
Target Protein Sequence
MFPEQQKEEF VSVWVRDPRI QKEDFWHSYI DYEICIHTNS MCFTMKTSCV RRRYREFVWL RQRLQSNALL VQLPELPSKN LFFNMNNRQH VDQRRQGLED FLRKVLQNAL LLSDSSLHLF LQSHLNSEDI EACVSGQTKY SVEEAIHKFA LMNRRFPEED EEGKKENDID YDSESSSSGL GHSSDDSSSH GCKVNTAPQE S
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Probable phosphoinositide-binding protein involved in protein sorting and membrane trafficking in endosomes. Plays a role in cilium biogenesis through regulation of the transport and the localization of proteins to the cilium. Required for the localization to the cilium of V-ATPase subunit ATP6V1D and ATP6V0D1, and RAB8A. Involved in osteoclast differentiation and therefore bone resorption.
Gene References into Functions
  1. supplementation with calcium gluconate rescued mice from the rachitic phenotype and extended life span in global Snx10-deficient mice, suggesting that this may be a life-saving component of the clinical approach to Snx10-dependent human osteopetrosis PMID: 25811986
  2. Data suggest Tyr32 and Arg51 in SNX10 are important for protein stability and play critical roles in vacuolation in osteoclasts; mutation Arg16Leu (seen in autosomal recessive osteopetrosis patients) affects protein-protein interactions of SNX10. PMID: 25212774
  3. results confirm the involvement of the SNX10 gene in human ARO and identify a new subset with a relatively favorable prognosis as compared to TCIRG1-dependent cases PMID: 23280965
  4. Structure of sorting nexin 11 (SNX11) reveals a novel extended phox homology (PX) domain critical for inhibition of SNX10-induced vacuolation. PMID: 23615901
  5. Since inhibition of vesicular trafficking is essential for osteoclast formation and activity and SNX10 is involved in vesicular trafficking, these studies may identify a new gene involved in the development of bone diseases including osteoporosis. PMID: 22174188
  6. Identification of SNX10 as a new osteopetrosis associated gene in consanguineous families of Palestinian origin. PMID: 22499339
  7. SNX10 regulates the ciliary trafficking of Rab8a, which is a critical regulator of ciliary membrane extension. PMID: 21844891
  8. SNX10 activity may be involved in the regulation of endosome homeostasis PMID: 17012226

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Involvement in disease
Osteopetrosis, autosomal recessive 8 (OPTB8)
Subcellular Location
Cytoplasm. Endosome membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=May also localize to nucleus and endoplasmic reticulum.
Protein Families
Sorting nexin family
Database Links

HGNC: 14974

OMIM: 614780

KEGG: hsa:29887

STRING: 9606.ENSP00000343709

UniGene: Hs.741316

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