Purity
Greater than 90% as determined by SDS-PAGE.
Alternative Names
CISH 1; CISH1; Cytokine inducible SH2 protein 1; JAB; JAK binding protein; JAK-binding protein; Janus kinase binding protein ; SOCS 1; SOCS-1; Socs1; SOCS1_HUMAN; SSI 1; SSI-1; SSI1; STAT induced STAT inhibitor 1; STAT-induced STAT inhibitor 1; Suppressor of cytokine signaling 1; Supressor of cytokine signalling 1; TEC interacting protein 3; Tec-interacting protein 3; TIP 3; TIP-3; TIP3
Species
Homo sapiens (Human)
Expression Region
1-211aa
Target Protein Sequence
MVAHNQVAADNAVSTAAEPRRRPEPSSSSSSSPAAPARPRPCPAVPAPAPGDTHFRTFRSHADYRRITRASALLDACGFYWGPLSVHGAHERLRAEPVGTFLVRDSRQRNCFFALSVKMASGPTSIRVHFQAGRFHLDGSRESFDCLFELLEHYVAAPRRMLGAPLRQRRVRPLQELCRQRIVATVGRENLARIPLNPVLRDYLSSFPFQI
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Full Length
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
The preparation of this recombinant Human SOCS1 protein was to use gene recombination DNA technology to obtain a recombinant vector connected with a SOCS1 fragment (1-211aa) that could be translated into the SOCS1 protein and then transferred it into E.coli cells to express the recombinant SOCS1 protein molecule. In order to get the target protein with high purity, N-terminal 6xHis tag was used in the production. The purity is 90% determined by SDS-PAGE.
SOCS1 is a member of the STAT-induced STAT inhibitor (SSI), also known as suppressor of cytokine signaling (SOCS), family. It is highly conserved among various species, including human, chimpanzee, dog, cow, rat and chicken. SOCS1 was also involved in E3 ubiquitin ligases complex, acting as substrate-recognition modules to mediate the polyubiquitination and subsequent degradation of substrate proteins which may be the key components of cytokine signal transduction pathways. Both two functions of SOC1 are very important to regulate the growth and proliferation of cell. Therefore, numerous studies have speculated that SOCS1 may play a role in regulating tumor growth and proliferation, such as hepatocellular carcinoma, melanoma, gastric cancer, prostate cancer and so on.
