Recombinant Human Transcriptional enhancer factor TEF-1(TEAD1)

In Stock
Code CSB-YP023363HU
Size US$1298Purchase it in Cusabio online store
(only available for customers from the US)
Image
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of CSB-YP023363HU could indicate that this peptide derived from Yeast-expressed Homo sapiens (Human) TEAD1.
  • Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of CSB-YP023363HU could indicate that this peptide derived from Yeast-expressed Homo sapiens (Human) TEAD1.

Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names TEAD1
Uniprot No. P28347
Research Area Cardiovascular
Alternative Names NTEF-1;Protein GT-IICTEA domain family member 1 ;TEAD-1Transcription factor 13 ;TCF-13
Species Homo sapiens (Human)
Source Yeast
Expression Region 1-426aa
Target Protein Sequence MEPSSWSGSESPAENMERMSDSADKPIDNDAEGVWSPDIEQSFQEALAIYPPCGRRKIILSDEGKMYGRNELIARYIKLRTGKTRTRKQVSSHIQVLARRKSRDFHSKLKDQTAKDKALQHMAAMSSAQIVSATAIHNKLGLPGIPRPTFPGAPGFWPGMIQTGQPGSSQDVKPFVQQAYPIQPAVTAPIPGFEPASAPAPSVPAWQGRSIGTTKLRLVEFSAFLEQQRDPDSYNKHLFVHIGHANHSYSDPLLESVDIRQIYDKFPEKKGGLKELFGKGPQNAFFLVKFWADLNCNIQDDAGAFYGVTSQYESSENMTVTCSTKVCSFGKQVVEKVETEYARFENGRFVYRINRSPMCEYMINFIHKLKHLPEKYMMNSVLENFTILLVVTNRDTQETLLCMACVFEVSNSEHGAQHHIYRLVKD
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 49.9kDa
Protein Length Full Length
Tag Info N-terminal 6xHis-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA Please contact us to get it.
Still Have Questions? Leave a Message or Start an on-line Chat

Q&A and Customer Reviews

 Q&A
Q:

I have a question about TEAD1 (CSB-YP023363HU).
Is it possible to request this product in PBS instead of the Tris-based buffer it is normally supplied in?

A:
Thanks for your inquiry,
We could use PBS as storage buffer. Please kindly remark this requirement when send your PO. We need to tell you in advance that we're not sure whether this protein can mantain stable in PBS. If this protein is unstable in this buffer, we will deliver this protein in default buffer (Tris buffer with 50% glycerol).
Q:

Is there an option to elute the protein into water and then lyophilize it, so that there is no salt in the protein? Additionally, would there be an additional charge?

A:
Thanks for your inquiry. For CSB-YP023363HU, we don't suggest to elute the protein into water and then lyophilize it, because the protein will be unstable if there is no suitable PH and salt ion concentration.
For the "salt" you mentioned, if you refer to Nacl, we could guarantee no salt in the protein. We could use tris-based buffer or HEPES. Please kindly confirm with the customer which buffer they want. As long as the protein is stable, we could replace any buffer that customer specifies.
You only need to remark this requirement before placing your order. There is no extra cost.
 Customer Reviews

Target Data

Function Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds specifically and cooperatively to the SPH and GT-IIC 'enhansons' (5'-GTGGAATGT-3') and activates transcription in vivo in a cell-specific manner. The activation function appears to be mediated by a limiting cell-specific transcriptional intermediary factor (TIF). Involved in cardiac development. Binds to the M-CAT motif.
Involvement in disease Sveinsson chorioretinal atrophy (SCRA)
Subcellular Location Nucleus
Tissue Specificity Preferentially expressed in skeletal muscle. Lower levels in pancreas, placenta, and heart.
Database Links

HGNC: 11714

OMIM: 108985

KEGG: hsa:7003

STRING: 9606.ENSP00000354588

UniGene: Hs.655331

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