Recombinant Human herpesvirus 1 ICP47 protein (US12)

The fusion tag N-terminal 6xHis tag gene was added to the gene sequence corresponding to the yeast of the HHV-1 US12 protein to form the recombinant DNA. The recombinant DNA was cloned into the expression vector and then transfected into the yeast cells for expression. Following purification, the product is the recombinant HHV-1 US12 protein carrying N-terminal 6xHis tag. The SDS-PAGE assessed the purity of this recombinant US12 protein up to 90%. It had an apparent molecular weight of approximately 14 kDa.

US12 is gene providing instructions for making a protein named human herpesvirus 1 ICP47 protein (abbreviated US12) and belongs to Herpesviridae US12 family. US12, a HSV immediate early protein, is small protein produced by the herpes simplex virus, is considered as an important factor in the evasion of cellular immune responses in HSV-infected cells. It is expressed very early after infection and can inhibit CD8+ T cell recognition of infected cells. Recent studies have established clearly that ICP47 can bind with high affinity to human TAP and inhibit the transport of peptides into the endoplasmic reticulum.