Product Details

Purity

Greater than 95% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/ug as determined by LAL method.

Activity

Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis TPBG at 2 μg/mL can bind Anti-TPBG recombinant antibody (CSB-RA024093MA1HU), the EC50 is 1.672-2.181 ng/mL.

Target Names

TPBG

Uniprot No.

Q4R8Y9

Research Area

Cancer

Alternative Names

(Wnt-activated inhibitory factor 1)(WAIF1)

Molecular Characterization

Species

Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)

Source

Mammalian cell

Expression Region

35-355aa

Target Protein Sequence

TSSASSSSSSAPFLASAASAQPPLPDQCPALCECSEAARTVKCVNRNLTEVPTDLPLYVRNLFLTGNQLAVLPAGAFARRPPLAELAALNLSGSRLDEVRGGAFEHLPSLRQLDLSHNPLAYLSPFAFSGSNASISAPSPLVELILNHIVPPDDKRQNRSFEGMVAAALVAGRALQGLHLLELASNHFLYLPRDVLAQLPSLRYLDLSNNSLVSLTYVSFRNLTHLESLHLEDNALKVLHNGTLAELQGLPHVRVFLDNNPWVCDCHMADMVTWLKQTGVVQGKDRLTCAFPEKMRNRVLLELNSADLDCDPILPPSLQTS

Mol. Weight

39.9 kDa

Protein Length

Partial

Tag Info

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

TPBG (also known as WAIF1) plays a notable role in Wnt signaling modulation and is actively investigated as a tumor-associated antigen in oncology research, making a cynomolgus monkey ortholog particularly valuable for preclinical antibody cross-reactivity screening. This recombinant partial construct, spanning residues 35–355, is expressed in mammalian cells to preserve native-like glycosylation and folding critical for accurate binding studies. Functional ELISA confirms robust activity, with immobilized protein at 2 μg/mL binding an anti-TPBG recombinant antibody at an EC50 of 1.672–2.181 ng/mL, supporting use in antibody development, epitope binning, and species cross-reactivity assessments. The protein carries N-terminal 10×His and C-terminal Myc tags for flexible detection and purification strategies, while purity exceeding 95% by SDS-PAGE and endotoxin levels below 1.0 EU/μg satisfy the criteria typical for functional cell-based assays and quantitative binding experiments.

Target Background

Function

May function as an inhibitor of Wnt/beta-catenin signaling by indirectly interacting with LRP6 and blocking Wnt3a-dependent LRP6 internalization.

Subcellular Location

Cell membrane; Single-pass type I membrane protein.

Database Links

KEGG: mcf:102132149

UniGene: Mfa.1408

Code	CSB-MP024093MOV
Abbreviation	Recombinant Cynomolgus monkey TPBG protein, partial (Active)
MSDS	MSDS Datasheet
Size	$9.9
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Activity Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis TPBG at 2 μg/mL can bind Anti-TPBG recombinant antibody (CSB-RA024093MA1HU), the EC₅₀ is 1.672-2.181 ng/mL.
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Recombinant Macaca fascicularis Trophoblast glycoprotein (TPBG), partial (Active)

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