Recombinant Macaca fascicularis ADAM metallopeptidase domain 9 (ADAM9), partial (Active)

ADAM9 metalloprotease plays critical roles in ectodomain shedding and cell-surface remodeling, making it a key target in neuroscience research focused on synaptic plasticity and neuroinflammation. This cynomolgus monkey ortholog, spanning residues 29–698 with a C-terminal 10xHis tag, demonstrates specific antibody binding in functional ELISA with an EC50 of 1.329–1.813 ng/mL, confirming proper folding and epitope accessibility that supports its use in inhibitor screening assays and IC50 determination studies. The greater than 95% purity determined by SDS-PAGE, combined with endotoxin levels below 1.0 EU/μg, meets the quality thresholds commonly required for kinetic parameter analysis including Km and Vmax measurements, where contaminants would otherwise confound enzymatic rate calculations. Expressed in mammalian cells to preserve native glycosylation patterns, this protein provides a suitable basis for substrate specificity profiling and serves as a positive control in enzyme-linked assays investigating ADAM9-mediated proteolytic events in primate neurological models.