Purity

≥ 95% as determined by SDS-PAGE.
≥ 95% as determined by SEC-HPLC.

Endotoxin

Less than 1.0 EU/ug as determined by LAL method.

Activity

Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis LRRC15 at 2 μg/mL on an Nickel Coated plate can bind Anti-LRRC15 recombinant antibody (CSB-RA819484MA1HU). The EC50 is 4.100-5.074 ng/mL.

Target Names

LRRC15

Uniprot No.

G7NYR2

Alternative Names

Leucine rich repeat containing 15; LRRC15; EGM_11224

Species

Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)

Source

Mammalian cell

Expression Region

22-538aa

Target Protein Sequence

YYGCPSECTCSRASQVECTGARIVAVPTPLPWNAMSLQILNTHITELSESPFLNISALIALRIEKNELSHIMPGAFRNLGSLRYLSLANNKLQVLPIGLFQGLDSLESLLLSSNQLVQIQPAHFSQCSNLKELQLHGNHLEYIPDGAFDHLVGLTKLNLGKNSLTHISPRVFQHLGNLQVLRLYENRLTDIPMGTFDGLVNLQELALQQNQIGLLSPGLFHNNHNLQRLYLSNNHISQLPPSIFMQLPQLNRLTLFGNSLKELSPGIFGPMPNLRELWLYDNHITSLPDNVFSNLRQLQVLILSRNQISFISPGAFNGLTELRELSLHTNALQDLDGNVFRMLANLQNISLQNNRLRQLPGNIFANVNGLMTIQLQNNQLENLPLGIFDHLGKLCELRLYDNPWRCDSDILPLRNWLLLNQPRLGTDTVPVCFSPANVRGQSLIIINVNVAVPSVHVPEVPSYPETSWYPDTSSYPDTTSISSTTELTSPVEDYTDLTTIQVTDDRSVWGMTQAQSG

Mol. Weight

59.4 kDa

Protein Length

Partial

Tag Info

C-terminal 10xHis-tagged

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

LRRC15 is emerging as a stromal marker in cancer-associated fibroblasts and a potential target for tumor microenvironment-directed therapies, making well-characterized reagents essential for antibody development and ligand discovery. This mammalian-expressed protein spanning residues 22–538 captures the extracellular leucine-rich repeat domain architecture required for native receptor conformation and antibody epitope presentation. Functional ELISA validation demonstrates specific antibody binding with an EC50 of 4.100–5.074 ng/mL, directly supporting its use in therapeutic antibody epitope mapping, competitive inhibition screening, and blocking antibody validation assays. The protein meets quality thresholds commonly required for surface plasmon resonance and biolayer interferometry affinity characterization, with purity exceeding 95% by SDS-PAGE and endotoxin levels below 1.0 EU/μg, making it appropriate for drug discovery campaigns targeting stromal interactions in the tumor microenvironment.

Code	CSB-MP5071MOV
Abbreviation	Recombinant Cynomolgus monkey LRRC15 protein, partial (Active)
MSDS	MSDS Datasheet
Size	$138
	Order now
Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Activity Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis LRRC15 at 2 μg/ml on an Nickel Coated plate can bind Anti-LRRC15 recombinant antibody (CSB-RA819484MA1HU). The EC₅₀ is 4.100-5.074 ng/mL. Biological Activity Assay The purity of LRRC15 was greater than 95% as determined by SEC-HPLC
Have Questions?	Leave a Message or Start an on-line Chat

Recombinant Macaca fascicularis Leucine rich repeat containing 15 (LRRC15), partial (Active)

Product Details

Customer Reviews and Q&A

×CloseMessage