LRRC15

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
Measured by its binding ability in a functional ELISA. Immobilized Human LRRC15 at 2 μg/ml on an Nickel Coated plate can bind Anti-LRRC15 recombinant antibody (CSB-RA819484MA1HU). The EC₅₀ is 12.18-15.75 ng/mL.

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Immunofluorescence staining of SH-SY5Y cells with CSB-PA819484LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunohistochemistry of paraffin-embedded human heart tissue using CSB-PA819484LA01HU at dilution of 1:100