Recombinant Macaca fascicularis Thymic stromal lymphopoietin (TSLP) (R127A,R130S) (Active)

TSLP drives type 2 immune responses by activating dendritic cells and promoting Th2 differentiation, making it a central mediator in allergic inflammation and asthma pathogenesis. This cynomolgus variant carries R127A and R130S substitutions and spans the full mature sequence (aa 29–159), enabling direct comparison with wild-type TSLP in structure-function studies and therapeutic antibody screening. Functional ELISA demonstrates antibody binding with an EC50 of 45.77–53.37 ng/mL, while biolayer interferometry confirms high-affinity interaction (KD = 4.41 nM), supporting use in antibody development workflows including epitope mapping, candidate ranking, and neutralization assays. The protein meets the purity and endotoxin criteria typical for cell-based assays (>95% pure, <1.0 EU/μg), providing a suitable basis for PBMC activation studies, dendritic cell maturation experiments, and JAK/STAT signaling pathway analysis where endotoxin contamination would confound cytokine-specific responses.