Recombinant Mouse [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4, mitochondrial (Pdk4), partial

Code CSB-YP017730MO
MSDS
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Source Yeast
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Code CSB-EP017730MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP017730MO
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP017730MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Pdk4
Uniprot No.
Alternative Names
Pdk4; [Pyruvate dehydrogenase; acetyl-transferring)] kinase isozyme 4; mitochondrial; EC 2.7.11.2; Pyruvate dehydrogenase kinase isoform 4
Species
Mus musculus (Mouse)
Expression Region
-
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Inhibition of pyruvate dehydrogenase decreases glucose utilization and increases fat metabolism in response to prolonged fasting and starvation. Plays an important role in maintaining normal blood glucose levels under starvation, and is involved in the insulin signaling cascade. Via its regulation of pyruvate dehydrogenase activity, plays an important role in maintaining normal blood pH and in preventing the accumulation of ketone bodies under starvation. In the fed state, mediates cellular responses to glucose levels and to a high-fat diet. Regulates both fatty acid oxidation and de novo fatty acid biosynthesis. Plays a role in the generation of reactive oxygen species. Protects detached epithelial cells against anoikis. Plays a role in cell proliferation via its role in regulating carbohydrate and fatty acid metabolism.
Gene References into Functions
  1. In conclusion, our data indicated that PDK4 potentially contributes to the hepatic steatosis in NASH via regulating several signaling pathway and PDK4 may be a new therapeutic strategy against NAFLD. PMID: 29128353
  2. our results suggest that PDK2/4 can be a potential target for the development of pharmacotherapy for the treatment of acute inflammatory pain PMID: 26931482
  3. Taken together these data support a model where PDHK4 regulates KRAS signalling and its tumorigenic properties and suggest that inhibition of PDHK4 could represent a novel therapeutic strategy to target KRAS mutant colorectal and lung cancers PMID: 28692044
  4. PDK4 mediates cisplatin-induced acute kidney injury. PMID: 28040265
  5. Pyruvate dehydrogenase kinase 4 is a direct transcriptional target of FoxO1 in the heart. FoxO1 directly regulates Pdk4 transcription in the heart, thereby controlling PDH activity and subsequent glucose oxidation rates. PMID: 28687587
  6. inhibition of PDK4 activity in Hepatocellular carcinoma cells increased cyclin E1, cyclin A2, and E2F1 proteins. PMID: 28003426
  7. FXR may promote the proliferation of tumor cells and the hepatocytes in the process of liver regeneration by activating the PDK4-mediated metabolic reprogramming to generate glycolytic intermediates essential for rapid biomass generation, establishing a mechanistic link between cell proliferation and metabolic switch. PMID: 26728993
  8. Taken together, our results suggest that LPS induces PDK4 expression and alters glucose metabolism via the JNK pathway. PMID: 26740179
  9. upregulation of PDK4 promotes vascular calcification by increasing osteogenic markers with no adverse effect on bone formation, demonstrating that PDK4 is a therapeutic target for vascular calcification. PMID: 26560812
  10. PDK2/4 induction and the subsequent lactate surge induce the metabolic shift in the diabetic dorsal root ganglion thereby contributing to the pathogenesis of painful diabetic neuropathy. PMID: 26769971
  11. These findings reveal that there are divergent roles of PDKs during oocyte maturation and indicate a new mechanism controlling meiotic structure. PMID: 25991547
  12. Double-knockout mice with global deletion of PDK2 and PDK4, which results in constitutively activated pyruvate dehydrogenase, preferentially oxidize glucose in muscle. PMID: 25368185
  13. PDK4 promotes tumorigenesis through activation of the CREB-RHEB-mTORC1 signaling cascade. PMID: 25164809
  14. Diminished insulin signaling evident at 1 wk on the high fat diet did not occur in pyruvate dehydrogenase kinase 4 knockout mice. PMID: 24116221
  15. The Pdk4 gene knockdown led to better glucose tolerance than the Pdk2 gene knockdown. Hepatic Pdk4 may be critically involved in the pathogenesis of diabetes. PMID: 23940800
  16. These results suggest a role for PDK4 in regulating the PDH complex in muscle and promoting gluconeogenic precursor recirculation during recovery from exhaustive exercise. PMID: 24305065
  17. loss of both Pdk2 and Pdk4 attenuated HSC quiescence, glycolysis, and transplantation capacity. PMID: 23290136
  18. Deletion of PDK4 prevented Angiotensin II-induced diastolic dysfunction and normalized glucose oxidation to basal levels. PMID: 23396452
  19. the role of IL-6 signalling in adipose tissue during exercise PMID: 22844518
  20. Data suggest that up-regulation of cardiac PDK4 by high-fat diet (HFD) occurs in conjunction with time-dependent activation of E2F1 transcription factor (E2F1); HFD may initiate early cardiac metabolic alterations through cyclin D1/E2F1/PDK4 axis. PMID: 22569253
  21. PDK4 plays a role in reducing pyruvate dehydrogenase complex activation during low to moderate-intensity muscle stimulation, and that absence of PDK4 and the subsequent changes in carbohydrate utilization may alter force production. PMID: 22196220
  22. These findings indicate that Pdk4 plays an important role in bone loss at unloading by promoting osteoclastogenesis. PMID: 21803180
  23. PDK4 is a novel pivotal factor in adaptation to chemical stress. PMID: 21182459
  24. upregulation of PDK4 in skeletal muscle PMID: 12099888
  25. activation of FXR may suppress glycolysis and enhance oxidation of fatty acids via inactivation of the pyruvate dehydrogenase complex by increasing PDK4 expression PMID: 15721319
  26. PGC-1alpha expression in skeletal muscle coordinately upregulate the expression of pyruvate dehydrogenase kinase 4 (PDK4), a negative regulator of glucose oxidation. PMID: 16314495
  27. Pyruvate dehydrogenase kinase 4-deficient mice have lower fasting blood glucose levels, slightly improved glucose tolerance, and slightly greater insulin sensitivity compared with wild-type mice. PMID: 18430968
  28. PDK4 upregulation in adipocytes participates in the hypolipidemic effect of thiazolidinediones through modulation of glyceroneogenesis PMID: 18519799
  29. Regulation of the PDK4 isozyme by the Rb-E2F1 complex PMID: 18667418
  30. PDHK4 deficiency creates conditions that alter upstream signalling components involved in the regulation of lipid metabolism. PMID: 19627255
  31. The activation of pyruvate dehydrogenase kinase 4 during starvation is mediated by peroxisome proliferator-activated receptor alpha PMID: 11554740

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Subcellular Location
Mitochondrion matrix.
Protein Families
PDK/BCKDK protein kinase family
Database Links
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