Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

Pdk4

Uniprot No.

O70571

Research Area

Metabolism

Alternative Names

Pdk4; [Pyruvate dehydrogenase; acetyl-transferring)] kinase isozyme 4; mitochondrial; EC 2.7.11.2; Pyruvate dehydrogenase kinase isoform 4

Species

Mus musculus (Mouse)

Source

E.coli

Expression Region

138-368aa

Target Protein Sequence

ILEYKDTCTVDPVTNQNLQYFLDRFYMNRISTRMLMNQHILIFSDSKTGNPSHIGSIDPNCDVVAVVQDAFECAKMLCDQYYLTSPELNLTQVNGKFPGQPIHIVYVPSHLHHMLFELFKNAMRATVEHQENRPSLTPVEATVVLGKEDLTIKISDRGGGVPLRITDRLFSYTYSTAPTPVMDNSRNAPLAGFGYGLPISRLYAKYFQGDLNLYSMSGYGTDAIIYLKALS
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

30.0 kDa

Protein Length

Partial

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Our Recombinant Mouse Pdk4 protein is expertly designed for researchers studying metabolism in mouse models. This protein features the pyruvate dehydrogenase (acetyl-transferring) kinase isozyme 4, a mitochondrial enzyme crucial for the regulation of the pyruvate dehydrogenase complex activity. The offered protein focuses on a partial region of Pdk4 with an expression range of 138-368aa, ensuring optimal functionality and consistency in your research endeavors.

Produced in an E. coli expression system, our Mouse Pdk4 protein is fused with an N-terminal 6xHis tag, facilitating efficient purification and detection procedures. With a purity greater than 85% as determined by SDS-PAGE, our recombinant protein is available in both liquid and lyophilized powder forms, accommodating various experimental needs. Trust our precision-engineered Recombinant Mouse Pdk4 to deliver consistent and reliable results for your metabolism research projects.

Target Background

Function

Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Inhibition of pyruvate dehydrogenase decreases glucose utilization and increases fat metabolism in response to prolonged fasting and starvation. Plays an important role in maintaining normal blood glucose levels under starvation, and is involved in the insulin signaling cascade. Via its regulation of pyruvate dehydrogenase activity, plays an important role in maintaining normal blood pH and in preventing the accumulation of ketone bodies under starvation. In the fed state, mediates cellular responses to glucose levels and to a high-fat diet. Regulates both fatty acid oxidation and de novo fatty acid biosynthesis. Plays a role in the generation of reactive oxygen species. Protects detached epithelial cells against anoikis. Plays a role in cell proliferation via its role in regulating carbohydrate and fatty acid metabolism.

Gene References into Functions

In conclusion, our data indicated that PDK4 potentially contributes to the hepatic steatosis in NASH via regulating several signaling pathway and PDK4 may be a new therapeutic strategy against NAFLD. PMID: 29128353
our results suggest that PDK2/4 can be a potential target for the development of pharmacotherapy for the treatment of acute inflammatory pain PMID: 26931482
Taken together these data support a model where PDHK4 regulates KRAS signalling and its tumorigenic properties and suggest that inhibition of PDHK4 could represent a novel therapeutic strategy to target KRAS mutant colorectal and lung cancers PMID: 28692044
PDK4 mediates cisplatin-induced acute kidney injury. PMID: 28040265
Pyruvate dehydrogenase kinase 4 is a direct transcriptional target of FoxO1 in the heart. FoxO1 directly regulates Pdk4 transcription in the heart, thereby controlling PDH activity and subsequent glucose oxidation rates. PMID: 28687587
inhibition of PDK4 activity in Hepatocellular carcinoma cells increased cyclin E1, cyclin A2, and E2F1 proteins. PMID: 28003426
FXR may promote the proliferation of tumor cells and the hepatocytes in the process of liver regeneration by activating the PDK4-mediated metabolic reprogramming to generate glycolytic intermediates essential for rapid biomass generation, establishing a mechanistic link between cell proliferation and metabolic switch. PMID: 26728993
Taken together, our results suggest that LPS induces PDK4 expression and alters glucose metabolism via the JNK pathway. PMID: 26740179
upregulation of PDK4 promotes vascular calcification by increasing osteogenic markers with no adverse effect on bone formation, demonstrating that PDK4 is a therapeutic target for vascular calcification. PMID: 26560812
PDK2/4 induction and the subsequent lactate surge induce the metabolic shift in the diabetic dorsal root ganglion thereby contributing to the pathogenesis of painful diabetic neuropathy. PMID: 26769971
These findings reveal that there are divergent roles of PDKs during oocyte maturation and indicate a new mechanism controlling meiotic structure. PMID: 25991547
Double-knockout mice with global deletion of PDK2 and PDK4, which results in constitutively activated pyruvate dehydrogenase, preferentially oxidize glucose in muscle. PMID: 25368185
PDK4 promotes tumorigenesis through activation of the CREB-RHEB-mTORC1 signaling cascade. PMID: 25164809
Diminished insulin signaling evident at 1 wk on the high fat diet did not occur in pyruvate dehydrogenase kinase 4 knockout mice. PMID: 24116221
The Pdk4 gene knockdown led to better glucose tolerance than the Pdk2 gene knockdown. Hepatic Pdk4 may be critically involved in the pathogenesis of diabetes. PMID: 23940800
These results suggest a role for PDK4 in regulating the PDH complex in muscle and promoting gluconeogenic precursor recirculation during recovery from exhaustive exercise. PMID: 24305065
loss of both Pdk2 and Pdk4 attenuated HSC quiescence, glycolysis, and transplantation capacity. PMID: 23290136
Deletion of PDK4 prevented Angiotensin II-induced diastolic dysfunction and normalized glucose oxidation to basal levels. PMID: 23396452
the role of IL-6 signalling in adipose tissue during exercise PMID: 22844518
Data suggest that up-regulation of cardiac PDK4 by high-fat diet (HFD) occurs in conjunction with time-dependent activation of E2F1 transcription factor (E2F1); HFD may initiate early cardiac metabolic alterations through cyclin D1/E2F1/PDK4 axis. PMID: 22569253
PDK4 plays a role in reducing pyruvate dehydrogenase complex activation during low to moderate-intensity muscle stimulation, and that absence of PDK4 and the subsequent changes in carbohydrate utilization may alter force production. PMID: 22196220
These findings indicate that Pdk4 plays an important role in bone loss at unloading by promoting osteoclastogenesis. PMID: 21803180
PDK4 is a novel pivotal factor in adaptation to chemical stress. PMID: 21182459
upregulation of PDK4 in skeletal muscle PMID: 12099888
activation of FXR may suppress glycolysis and enhance oxidation of fatty acids via inactivation of the pyruvate dehydrogenase complex by increasing PDK4 expression PMID: 15721319
PGC-1alpha expression in skeletal muscle coordinately upregulate the expression of pyruvate dehydrogenase kinase 4 (PDK4), a negative regulator of glucose oxidation. PMID: 16314495
Pyruvate dehydrogenase kinase 4-deficient mice have lower fasting blood glucose levels, slightly improved glucose tolerance, and slightly greater insulin sensitivity compared with wild-type mice. PMID: 18430968
PDK4 upregulation in adipocytes participates in the hypolipidemic effect of thiazolidinediones through modulation of glyceroneogenesis PMID: 18519799
Regulation of the PDK4 isozyme by the Rb-E2F1 complex PMID: 18667418
PDHK4 deficiency creates conditions that alter upstream signalling components involved in the regulation of lipid metabolism. PMID: 19627255
The activation of pyruvate dehydrogenase kinase 4 during starvation is mediated by peroxisome proliferator-activated receptor alpha PMID: 11554740

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Subcellular Location

Mitochondrion matrix.

Protein Families

PDK/BCKDK protein kinase family

Database Links

KEGG: mmu:27273

STRING: 10090.ENSMUSP00000019721

UniGene: Mm.235547

Code	CSB-EP017730MO
Abbreviation	Recombinant Mouse Pdk4 protein, partial
MSDS	MSDS Datasheet
Size	US$306
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Mouse [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4,mitochondrial (Pdk4), partial

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