Recombinant Mouse A-kinase anchor protein 8-like (Akap8l)

Code CSB-YP889923MO
MSDS
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Source Yeast
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Code CSB-EP889923MO
MSDS
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Source E.coli
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Code CSB-EP889923MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP889923MO
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP889923MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Akap8l
Uniprot No.
Alternative Names
Akap8l; Nakap; Nakap95A-kinase anchor protein 8-like; AKAP8-like protein; Neighbor of A-kinase-anchoring protein 95; Neighbor of AKAP95
Species
Mus musculus (Mouse)
Expression Region
1-642
Target Protein Sequence
MSYTGFVQGS ETTLQSTYCD TSAQPTCDYG YGTWNSGTNR GYENYGYGYG YGQDNTTNYG YGMATSHSWE MASSDTNANP SASGSASADS VLSRINQRLD MMPHLETDMI QGGVYGSGGG ERYDSYEACD SRAILSERDL YRSSYDYGEL DPEMEMAYEG QYDAYRDQFR MRGGDTFGPR AQGWARDARS GRPMASGYGR MWEDPMGARG QCMPGASRLP SLFSQNIIPE YGMFQGMRGG GAFSGGSRFG FGFGNGMKQM RRTWKTWTTA DFRTKKKKRK QGGSPDEPDS KATRTDCSDN SDSDNDEGTE GEAAEGTESA EAMEKGSRVD GEDEEGKEDG REEGKEDPEK GALTAQDESS QAKRKLQASK KSQDKQKKRQ RDRMVERIQF VCSLCKYRTF YEDEMGSHLD SKFHKEHFKY VGTKLPKQTA DFLQEYVTNK TKKTEELRKT VEDLDGLIQQ IYRDQDLTQE IAMEHFVKKV EAAHCAACDL FIPMQFGIIQ KHLKTMDHNR NRRLMMEQSK KSSLMVARSI LNNKLISKKL ERYLKGENPF TDNPEEEKEQ DEVEAGALDE GAPSEATELT EGVPAQPPVP LEPAPGTTTP PPPPPPEEEE SPVPLLGGAL QCQIRGIPGL DMEDDEEGGG GP
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Could play a role in constitutive transport element (CTE)-mediated gene expression by association with DHX9. Increases CTE-dependent nuclear unspliced mRNA export. Proposed to target PRKACA to the nucleus but does not seem to be implicated in the binding of regulatory subunit II of PKA. May be involved in nuclear envelope breakdown and chromatin condensation. May be involved in anchoring nuclear membranes to chromatin in interphase and in releasing membranes from chromating at mitosis. May regulate the initiation phase of DNA replication when associated with TMPO isoform Beta. Required for cell cycle G2/M transition and histone deacetylation during mitosis. In mitotic cells recruits HDAC3 to the vicinity of chromatin leading to deacetylation and subsequent phosphorylation at 'Ser-10' of histone H3; in this function seems to act redundantly with AKAP8. May be involved in regulation of pre-mRNA splicing.
Subcellular Location
Nucleus. Nucleus matrix. Nucleus speckle. Nucleus, PML body. Cytoplasm.
Protein Families
AKAP95 family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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