Recombinant Mouse Scinderin (Scin)

Code CSB-YP713881MO
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP713881MO
MSDS
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Source E.coli
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Code CSB-EP713881MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP713881MO
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP713881MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Scin
Uniprot No.
Alternative Names
ScinAdseverin; Gelsolin-like protein; Scinderin
Species
Mus musculus (Mouse)
Expression Region
1-715
Target Protein Sequence
MAQELQHPEF ARAGQQAGLQ VWRVEKLELV PVPQGAYGDF YVGDAYLVLH TTKSSRGFSY RLHFWLGKEC SQDESTAAAI FTVQMDDYLG GKPVQSRELQ GYESTDFVGY FKGGLKYKAG GVASGLNHVL TNDLTAKRLL HVKGRRVVRA TEVPLSWESF NKGDCFIIDL GTEIYQWCGS SCNKYERLKA SQVAIGIRDN ERKGRSQLIV VEEGSEPSEL MKVLGRKPEL PDGDNDDDVV ADISNRKMAK LYMVSDASGS MKVTLVAEEN PFSMGMLLSE ECFILDHGAA KQIFVWKGKN ANPQERKTAM KTAEEFLQKM KYSTNTQIQV LPEGGETPIF KQFFKDWKDK DQSDGFGKVY ITEKVAQIKQ IPFDASKLHS SPQMAAQHNM VDDGSGGVEI WRVENSGRVQ IDPSSYGEFY GGDCYIILYT YPRGQIIYTW QGANATRDEL TMSAFLTVQL DRSLGGQAVQ VRVSQGKEPA HLLSLFKDKP LIIYKNGTSK KEGQAPAPPT RLFQVRRNLA SITRIVEVDV DANSLNSNDT FVLKLPRNNG FIWIGKGASQ EEEKGAEYVA DVLKCKASRI QEGKEPEEFW NSLGGRGDYQ TSPLLETRAE DHPPRLYGCS NKTGRFIIEE VPGEFTQDDL AEDDVMLLDA WEQIFIWIGK DANEVEKKES VKSAKMYLET DPSGRDKRTP IVIIKQGHEP PTFTGWFLGW DSSRW
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Ca(2+)-dependent actin filament-severing protein that has a regulatory function in exocytosis by affecting the organization of the microfilament network underneath the plasma membrane. Severing activity is inhibited by phosphatidylinositol 4,5-bis-phosphate (PIP2). In vitro, also has barbed end capping and nucleating activities in the presence of Ca(2+). Required for megakaryocyte differentiation, maturation, polyploidization and apoptosis with the release of platelet-like particles. Plays a role in osteoclastogenesis (OCG) and actin cytoskeletal organization in osteoclasts. Regulates chondrocyte proliferation and differentiation. Inhibits cell proliferation and tumorigenesis. Signaling is mediated by MAPK, p38 and JNK pathways.; fails to nucleate actin polymerization, although it severs and caps actin filaments in a Ca(2+)-dependent manner.
Gene References into Functions
  1. indicate that in healthy, developing tissues, adseverin contributes to the regulation of osteoclast structure but not to bone metabolism in vivo PMID: 28389691
  2. Data indicate that adseverin (Ads) regulates actin cytoskeleton in osteoclasts. PMID: 25681458
  3. Ads is a RANKL induced early regulator of osteoclastogenesis with a potential role in pre-osteoclast differentiation and fusion. PMID: 25275604
  4. adseverin induction is a specific gene regulatory effect by TCDD on thymocytes. PMID: 11883943
  5. Overexpression of Scin is associated with asthma. PMID: 19028979

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Subcellular Location
Cytoplasm, cytoskeleton. Cell projection, podosome.
Protein Families
Villin/gelsolin family
Tissue Specificity
Ubiquitous. Highly expressed in mature osteoclasts (at protein level). Isoform 2 is expressed in blood cells.
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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