Recombinant Mouse Crk-like protein (Crkl)

Code CSB-YP005980MO
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP005980MO
MSDS
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Source E.coli
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Code CSB-EP005980MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP005980MO
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP005980MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Crkl
Uniprot No.
Alternative Names
Crkl; CrkolCrk-like protein
Species
Mus musculus (Mouse)
Expression Region
1-303
Target Protein Sequence
MSSARFDSSD RSAWYMGPVT RQEAQTRLQG QRHGMFLVRD SSTCPGDYVL SVSENSRVSH YIINSLPNRR FKIGDQEFDH LPALLEFYKI HYLDTTTLIE PAPRYPSPPV GSVSAPNLPT AEENLEYVRT LYDFPGNDAE DLPFKKGELL VIIEKPEEQW WSARNKDGRV GMIPVPYVEK LVRSSPHGKH GNRNSNSYGI PEPAHAYAQP QTTTPLPTVA STPGAAINPL PSTQNGPVFA KAIQKRVPCA YDKTALALEV GDIVKVTRMN INGQWEGEVN GRKGLFPFTH VKIFDPQNPD DNE
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
May mediate the transduction of intracellular signals.
Gene References into Functions
  1. CRKL plays an important role in hepatocarcinoma cell proliferation, invasion and migration as well hepatocarcinoma malignancy and metastasis. PMID: 25960220
  2. CRKL is shown to act as a potential suppressor and to provide new insight for both the malignant behaviors of hepatocarcinoma cells and lymphatic metastasis mechanism of hepatocarcinoma. PMID: 25661331
  3. Sprouty2 acts as an inhibitor of CrkL-Rap1 signaling. PMID: 20136656
  4. The molecular signaling set off by ERalpha and CrkL association may have a central role in pregnancy and cancer. PMID: 21700719
  5. Reelin-induced Dab1 tyrosine phosphorylation may generate a multifaceted signaling scaffold containing a rich array of Crk/CrkL-SH3 binding effectors PMID: 21879738
  6. CrkL plays a specific role in integrin-induced migration as a downstream mediator of Src. PMID: 12665586
  7. CrkL is not absolutely required for T cell development or function. PMID: 14515252
  8. These results suggest a role for CrkL in the Reelin signaling pathway to control neuronal migration. PMID: 17394141
  9. Crkol is essential for cranial and cardiac neural crest development. The chromosomal location of the human CRKL gene (22q11.21) and the phenotype of Crkol mutant mice suggest that defects in CRKL-mediated pathways underlie DiGeorge syndrome. PMID: 11242111

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Protein Families
CRK family
Database Links
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