Recombinant Mouse E3 ubiquitin-protein ligase Mdm2(Mdm2)

Code CSB-EP013626MO
Size US$2466
Image
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Have Questions? Leave a Message or Start an on-line Chat

Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names Mdm2
Uniprot No. P23804
Research Area Others
Alternative Names Mdm2; E3 ubiquitin-protein ligase Mdm2; EC 2.3.2.27; Double minute 2 protein; Oncoprotein Mdm2; RING-type E3 ubiquitin transferase Mdm2; p53-binding protein Mdm2
Species Mus musculus (Mouse)
Source E.coli
Expression Region 1-489aa
Target Protein Sequence MCNTNMSVSTEGAASTSQIPASEQETLVRPKPLLLKLLKSVGAQNDTYTMKEIIFYIGQYIMTKRLYDEKQQHIVYCSNDLLGDVFGVPSFSVKEHRKIYAMIYRNLVAVSQQDSGTSLSESRRQPEGGSDLKDPLQAPPEEKPSSSDLISRLSTSSRRRSISETEENTDELPGERHRKRRRSLSFDPSLGLCELREMCSGGSSSSSSSSSESTETPSHQDLDDGVSEHSGDCLDQDSVSDQFSVEFEVESLDSEDYSLSDEGHELSDEDDEVYRVTVYQTGESDTDSFEGDPEISLADYWKCTSCNEMNPPLPSHCKRCWTLRENWLPDDKGKDKVEISEKAKLENSAQAEEGLDVPDGKKLTENDAKEPCAEEDSEEKAEQTPLSQESDDYSQPSTSSSIVYSSQESVKELKEETQDKDESVESSFSLNAIEPCVICQGRPKNGCIVHGKTGHLMSCFTCAKKLKKRNKPCPVCRQPIQMIVLTYFN
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 58.6kDa
Protein Length Full Length
Tag Info N-terminal 6xHis-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its degradation by the proteasome
Gene References into Functions
  1. The proximal p53 inhibitor MDM2 is markedly downregulated in subcutaneous white and brown adipose tissues of mice during aging. Genetic disruption of MDM2 in adipocytes triggers canonical p53-mediated apoptotic and senescent programs, leading to age-dependent lipodystrophy and its associated metabolic disorders, including type 2 diabetes, nonalcoholic fatty liver disease, hyperlipidemia, and energy imbalance. PMID: 30131393
  2. To investigate whether MDM2C462A, which retains p53 binding, has p53-suppressing activity, we generated Mdm2C462A/C462A;p53ER/- mice. Adult Mdm2-null mice died approximately 7 days after tamoxifen-induced p53 activation, indicating that in the absence of MDM2, MDMX cannot suppress p53. p53 activity is higher in the presence of MDM2C462A than in the absence of MDM2. PMID: 29123033
  3. These results show that ischemic preconditioning increased neuronal MDM2 protein levels, which prevented ischemia-induced p53 stabilization and neuronal death. PMID: 29371613
  4. the disruption of Mdm2/p53 interaction affects the early-embryonic otic progenitor cells and their descendants. PMID: 28181574
  5. E2F6 suppresses Mdm2 expression in cells harboring the SNP309G allele but not the SNP309T allele. PMID: 28925402
  6. the MDM2-p53-PC signalling axis links mitochondrial metabolism to insulin secretion and glucose homeostasis, and could represent a therapeutic target in diabetes. PMID: 27265727
  7. Selective dysregulation of Mdm2 and Mdm4 alternative splicing underlies p53 anti-repression and motor neuron death in a mouse model of spinal muscular atrophy. PMID: 30012555
  8. Aging mouse models have revealed the complexity of the p53-Mdm2 axis and have solidly placed the p53 network as being key to many aspects of both pathological aging conditions and normal aging (review). PMID: 29192902
  9. The results indicated that simultaneously knocking down MDM2 and overexpressing p53 was able to inhibit proliferation and induce G1 cell cycle arrest in H1299 cells, compared with either alone. PMID: 29039579
  10. These results illustrate the importance of the cooperative activities of p53 and Mdm2 in a network of miRNAs that function to impose a barrier against aberrant cardiomyocyte cell cycle re-entry to maintain cardiac homeostasis. PMID: 28745540
  11. c-Abl phosphorylation of Mdm2 has a role in regulation of p53 tumor suppression and bone marrow failure PMID: 27956626
  12. Bre enhances osteoblastic differentiation by promoting the Mdm2-mediated degradation of p53. PMID: 28436570
  13. genetic and biochemical data support a role for Mdm2 in cardiac growth control through the regulation of p53, the Pgc-1 family of transcriptional coactivators and the pivotal antioxidant Pink1 PMID: 29267372
  14. The availability of large-scale genomic profiling datasets, like those from The Cancer Genome Atlas Research Network, have provided the opportunity to evaluate the consequences of MDM2 amplification and SNP inheritance across high-quality tumor samples from diverse cancer indications. [review] PMID: 27194168
  15. findings document contrasting effects of ATM-Mdm2 signaling on p53 tumor suppression and reveal that destabilizing Mdm2 by promoting its phosphorylation by ATM would be effective in treating oncogene-induced malignancies. PMID: 27568562
  16. the existence of an unusual functional interplay between STATs and CREB at the onset of adipogenesis through shared CRTC cofactors, is reported. PMID: 27362806
  17. Mdm2 expression is required for cell survival even in the absence of p53. Moreover, results suggest that p73 compensates for loss of p53. PMID: 28576884
  18. In Fmr1 KO neurons, Mdm2 is hyperphosphorylated, nuclear localized basally, and unaffected by MEF2 activation, which our data suggest due to an enhanced interaction with Eukaryotic Elongation Factor 1alpha (EF1alpha), whose protein levels are elevated in Fmr1 KO. Expression of a dephosphomimetic of Mdm2 rescues PSD-95 ubiquitination, degradation and synapse elimination in Fmr1 KO neurons. PMID: 28025327
  19. MDM2 is a non-redundant survival factor for proximal tubular cells by protecting them from spontaneous p53 overexpression-related cell death. PMID: 27882940
  20. The case emphasizes that MDM2 expression represents a possible pitfall in the diagnosis of spindle cell tumors. The differential diagnostic distinction between FDCS and a dedifferentiated liposarcoma is discussed. PMID: 27271257
  21. MDM2 is involved in fibroblast activation, mediating renal tubulointerstitial fibrosis via a p53-independent pathway dependant on Notch1 ubiquitination and proteasome degradation. PMID: 28100501
  22. These findings suggest that Mdm2 splice isoforms may play critical roles in the regulatory loop of p53/Mdm2-Mdm4 via a RING domain-mediated biochemical mechanism. PMID: 28166445
  23. both MDM2 and MDMX deletion-caused pancreatic defects are completely rescued by loss of p53, verifying the crucial role of the MDM2 and/or MDMX in regulating p53 in a spatio-temporal manner during the development, functional maintenance, and related disease progress of endocrine pancreas. PMID: 28118981
  24. Vif stabilization by CBFbeta is mainly caused by impairing MDM2-mediated degradation. PMID: 27758855
  25. These results demonstrated a critical prosurvival role for MDM2 in the oocytes PMID: 27912078
  26. we failed to detect any increase in p53 level in mutant oocytes, nor any other apoptotic marker, introgression of this targeted invalidation in p53-/- mice restored the fertility of females. This study is the first to show that Mdm2, but not Mdm4, has a critical role in oocyte survival and would be involved in premature ovarian insufficiency phenotype. PMID: 27364741
  27. Inhibition of MDM2 re-sensitizes rapamycin resistant renal cancer cells via the activation of p53. PMID: 27825169
  28. Inactivation of Mdm2 in sertoli cells triggers p53 activation and apoptosis as early as 15.5 days post conception with a significant increase in apoptosis. PMID: 26470726
  29. These findings elucidate a critical role of Mdm2-p53-Nedd4-2 signaling underlying the regulation of neural network synchrony and seizure susceptibility. PMID: 27000207
  30. These studies demonstrate that Mdm2 holds promise as a therapeutic target in combination with conventional therapy and may lead to new clinical therapies for Triple-negative breast cancers . PMID: 26494859
  31. there is a fine tuned balance in the interaction of ribosomal proteins with the MDM2/p53 axis which is important in normal hematopoiesis. PMID: 27042854
  32. Study showed that demonstrate that Mdm2 intertwines the mammalian target of RAPA, mTOR, and the receptor kinase GRK2 in regulating the desensitization/inactivation of the GPR17 receptor PMID: 26228571
  33. MDM2 mediates p73 ubiquitination PMID: 26025930
  34. MDM2 supports the PRC2 mediated repression of lineage-specific genes in stem cells, independent of p53. PMID: 26748827
  35. physiological activation of the 5S RNP-Mdm2-p53 pathway may contribute to functional decline of the hematopoietic system in a cell-autonomous manner over time. PMID: 25987256
  36. Ribosomal proteins L11 and L5 activate TAp73 by overcoming MDM2 inhibition. PMID: 25301064
  37. The Mdm2(SNP309-)(G) allele significantly impacts CRC through mechanisms outside the p53 pathway. PMID: 25435368
  38. MDM2 maintains homeostasis and long survival in podocytes preventing apoptosis. PMID: 25349197
  39. our results show MDM4-MDM2/p53-IGF1R as an original regulatory mechanism for CNS regeneration PMID: 25981963
  40. Mir-660 inhibits lung tumorigenesis by targeting MDM2-p53 interaction. PMID: 25501825
  41. L-GILZ stabilizes p53 proteins by decreasing p53 ubiquitination and increasing MDM2 ubiquitination. PMID: 25168242
  42. Interaction of BAI1 with the N-terminus (AA 1-200) of MDM2 in the brain modulates PSD-95 levels and thereby regulates synaptic plasticity. PMID: 25751059
  43. Mdm2regulates entry into myogenesis by targeting CEBPb for degradation by the 26 S proteasome. PMID: 25720496
  44. Src phosphorylation converts Mdm2 from a ubiquitinating to a neddylating E3 ligase. PMID: 25624478
  45. Data show that NEDD4-1 E3 ligase as a novel component of the tumor suppressor protein p53/proto-oncogene proteins c-mdm2 regulatory feedback loop that controls p53 activity during stress responses. PMID: 24413081
  46. Restoration of wild-type p53 expression in Mdm2-overexpressing tumors suppresses tumor growth. PMID: 24598047
  47. results reveal a novel p53- and Mdm2-independent oncogenic function of Mdmx that provides new insight into the many cancers that overexpress Mdmx. PMID: 24608433
  48. both MDM2 and MDMX are required for monitoring p53 activity during lens development, and they may function independently or synergistically to control p53 and maintain normal lens morphogenesis PMID: 25263199
  49. The regulation of Mdm2 by the E3 ubiquitin ligase APC/C is shown. It has important therapeutic implications for tumors with Mdm2 overexpression. PMID: 24804778
  50. TSLP induces mast cell development and aggravates allergic reactions through the activation of MDM2 and STAT6. PMID: 24751726
  51. the action of MDM2 on HIF1alpha under hypoxia occurs in the cytoplasm and is controlled by the PTEN-PI3K-AKT signaling axis. PMID: 24982421
  52. The in vivo role of Mdm2 E3 ubiquitin ligase activity, believed to be a critical Mdm2 function in p53 regulation, is dispensable in embryogenesis and development in a mouse model. PMID: 25117711
  53. Mdm2 is essential for mouse viability at all ages. PMID: 24789767
  54. Data indicate that a full-dosage of p53 and an intact ribosomal protein-murine double minute 2 protein (Mdm2)-p53 pathway are required for the induction of malonyl coA decarboxylase (MCD), a critical regulator of fatty acid oxidation. PMID: 24872453
  55. Mdm2 regulates p53 in the adult lens in vivo. PMID: 24339722
  56. Mdm2 through its regulation of the pro-apoptotic, growth-suppressive protein, p53, is essential for the renewal/survival of nephron progenitor cells. PMID: 24440154
  57. MDM2 links inflammation and renal epithelial healing during acute kidney injury. PMID: 22297670
  58. This review focuses on the functions of mitochondrial p53 and alternative reading frame (ink4a/ARF) via interactions with mitochondrial proteins. PMID: 22998186
  59. MDM2-mediated mitotic catastrophe is a previously unrecognized variant of podocyte loss where MDM2 forces podocytes to complete the cell cycle, which in the absence of cytokinesis leads to podocyte aneuploidy, mitotic catastrophe, and loss by detachment. PMID: 23749457
  60. We provide information helpful for clarifying biomolecular responsive machinery present in 3T3-L1 adipocytes. PMID: 23682023
  61. Data indicate that double minute 2 (MDM2) was involved in miR-17-5p/20a regulation and function. PMID: 23333058
  62. Formation of HSP70- and MDM2-dependent protein coaggregates in tumours with high levels of these two proteins could be one of the mechanisms by which mutant p53 is stabilized. PMID: 23251530
  63. these results indicate that 1,25-dihydroxy vitamin D3 and its receptor have a role in the regulation of P2-initiated Mdm2 gene expression in a p53-dependent way. PMID: 23149414
  64. We show that Mdm2 binds to Nbs1 of the Mre11-Rad50-Nijmegen breakage syndrome (Nbs) 1 DNA repair complex. In addition, we provide evidence that Mdm2 inhibitors delay DNA repair. PMID: 23115126
  65. Mdm2 haploinsufficiency significantly delayed tumorigenesis in mice deficient in Arf and p53. PMID: 23029416
  66. These results define Mdm2 as a crucial regulator of capillary maintenance and exercise-induced angiogenesis in skeletal muscle. PMID: 22835827
  67. As Mdm2 is able to promote adipogenesis in the myoblast cell line C2C12, it is conceivable that Mdm2 acts as a switch in cell fate determination. PMID: 22388350
  68. Synergistic regulation of p53 by Mdm2 and Mdm4 is critical in cardiac endocardial cushion morphogenesis during heart development. PMID: 22821713
  69. Overexpression of p53 causes rapid SHP protein degradation, which does not require the presence of Mdm2 and is mediated by the proteosome pathway PMID: 22737255
  70. disruption of p53-MDM2 interaction by Nutlin-3a might reduce cell proliferation and promote apoptosis in osteosarcoma with MDM2 amplification and wt p53 status PMID: 22843172
  71. the role of Mdm2's RING domain in the control of p53 PMID: 22666487
  72. Mdm2 was depleted by infecteing with wtHAdV or a mutant virus lacking the E1B-55K gene (dl 1520). PMID: 22262167
  73. ATM phosphorylation of Mdm2 Ser394 governs p53 protein levels and functions in cells undergoing DNA damage. PMID: 22624716
  74. Mdm2 inhibitors not only trigger growth arrest, but may also stimulate p53's reported ability to slow homologous recombination repair. PMID: 21557332
  75. the mechanisms of radioprotection by GSK-3beta inhibitors in hippocampal neurons involve regulation of MDM2-dependent p53 accumulation and interactions between GSK-3beta, MDM2 and p53. PMID: 21738215
  76. NS undergoes a ubiquitin- and MDM2-independent proteasomal degradation when intracellular GTP levels are markedly reduced PMID: 22318725
  77. We observed greater expression and activation of Mdm2 in the spleen and kidneys in a mouse model of lupus (MRL-Fas(lpr) mice) than healthy controls. PMID: 21949095
  78. Data suggest that systemic inhibition of MdmX is both a feasible therapeutic strategy for restoring p53 function in tumors that retain wild-type p53 and likely to be significantly safer than inhibition of Mdm2. PMID: 21852537
  79. disruption of the RP-Mdm2-p53 pathway by an Mdm2(C305F) mutation does not accelerate prostatic tumorigenesis induced by inactivation of the pRb family proteins (pRb/p107/p130) PMID: 21747916
  80. Heterodimerization of Mdm2 and Mdm4 is critical for regulating p53 activity during embryogenesis but dispensable for p53 and Mdm2 stability. PMID: 21730132
  81. MDM2 and MDMX function as an integral complex in p53 control. PMID: 21730163
  82. a mechanism underlying the SENP2-mediated regulation of Mdm2 that is critical for genome integrity in p53-dependent stress responses. PMID: 21183956
  83. Trp53 regulates Notch 4 signaling through physical interactions with Mdm2. PMID: 21402876
  84. These results place MDM2 at a major nexus between the p53 and Shh signaling pathways in granular neuronal precursor. PMID: 21437245
  85. the ureteric bud (mdm2-/-) mutant phenotype is mediated by aberrant p53 activity because it is rescued by ureteric bud-specific deletion of the p53 gene PMID: 21420949
  86. Murine double minute clone 2 (MDM2)protein regulates estrogen receptor alpha and estrogen responsiveness in breast cancer cells. PMID: 21169420
  87. Mouse double minute 2 (Mdm2) gene links genotoxic stress and metabolism to tumor suppressor, p53. (Review) PMID: 21213101
  88. that NPC-related and cholesterol perturbation-induced axonal pathology is associated with an abnormal signaling pathway consisting in p38 MAPK activation leading to Mdm2-mediated p53 degradation, followed by ROCK activation PMID: 20386595
  89. activation of endogenous p53 by ablation of Mdm2 can induce accelerated aging phenotypes in mice. PMID: 21334322
  90. loss of CSN6 enhances p53-mediated tumor suppression in vivo and CSN6 plays an important role in regulating DNA damage-associated apoptosis and tumorigenesis through control of the MDM2-p53 signaling pathway PMID: 21317535
  91. novel evidence that the inhibition of Nrf2 can suppress Mdm2 expression, which may result in p53 signaling modulation PMID: 21211512
  92. Mdm2 is required to control reactive oxygen species-induced p53 levels for sustainable hematopoiesis PMID: 21040902
  93. the LIM domain protein Enigma directly interacts with MDM2 to form a ternary complex with p53 in embryonic fibroblasts PMID: 21060154
  94. results demonstrate a pathway for survival of activated T cells through NF-kappaB-induced Mdm2, which blocks Bim-dependent apoptosis through binding and inhibition of p73 PMID: 20921405
  95. Data show that although both Mdm2 and Mdm4 transgenes are designed to be expressed ubiquitously and at comparable levels, only the Mdm4 transgenic protein is produced at high levels in vivo. PMID: 20855528
  96. overall negative control of MDM4 by p76(MDM2) reflects on p53 function as p76(MDM2) impairs MDM4-mediated inhibition of p53 activity PMID: 20697359
  97. E1B-55kD-deleted oncolytic adenovirus carrying mutant KRAS-regulated hdm2 transgene exerts specific antitumor efficacy on colorectal cancer cells PMID: 20124454
  98. These data provide a possible mechanism on how p53 regulate HBx stability and also a new clue for the study of p53 mutation and hepatocellular carcinoma development. PMID: 19842060
  99. MDM2 mediates ubiquitination and degradation of activating transcription factor 3. PMID: 20592017
  100. SCYL1-BP1 can be ubiquitinated and degraded by Pirh2 but not by MDM2, which suggests that SCYL1-BP1 can be regulated by Pirh2. PMID: 20598683

Show More

Hide All

Subcellular Location Nucleus, nucleoplasm, Cytoplasm, Nucleus, nucleolus
Protein Families MDM2/MDM4 family
Tissue Specificity Ubiquitously expressed at low-level throughout embryo development and in adult tissues. MDM2-p90 is much more abundant than MDM2-p76 in testis, brain, heart, and kidney, but in the thymus, spleen, and intestine, the levels of the MDM2 proteins are roughly
Database Links

KEGG: mmu:17246

STRING: 10090.ENSMUSP00000020408

UniGene: Mm.22670

Most popular with customers

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

© 2007-2020 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1