Recombinant Mouse EGF-like repeat and discoidin I-like domain-containing protein 3 (Edil3)

Code CSB-YP007399MO
MSDS
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Source Yeast
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Code CSB-EP007399MO
MSDS
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Source E.coli
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Code CSB-EP007399MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP007399MO
MSDS
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Source Baculovirus
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Code CSB-MP007399MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Edil3
Uniprot No.
Alternative Names
Edil3; Del1EGF-like repeat and discoidin I-like domain-containing protein 3; Developmentally-regulated endothelial cell locus 1 protein; Integrin-binding protein DEL1
Species
Mus musculus (Mouse)
Expression Region
24-480
Target Protein Sequence
DICNPNP CENGGICLSG LADDSFSCEC PEGFAGPNCS SVVEVASDEE KPTSAGPCIP NPCHNGGTCE ISEAYRGDTF IGYVCKCPRG FNGIHCQHNI NECEAEPCRN GGICTDLVAN YSCECPGEFM GRNCQYKCSG PLGIEGGIIS NQQITASSTH RALFGLQKWY PYYARLNKKG LINAWTAAEN DRWPWIQINL QRKMRVTGVI TQGAKRIGSP EYIKSYKIAY SNDGKTWAMY KVKGTNEEMV FRGNVDNNTP YANSFTPPIK AQYVRLYPQI CRRHCTLRME LLGCELSGCS EPLGMKSGHI QDYQITASSV FRTLNMDMFT WEPRKARLDK QGKVNAWTSG HNDQSQWLQV DLLVPTKVTG IITQGAKDFG HVQFVGSYKL AYSNDGEHWM VHQDEKQRKD KVFQGNFDND THRKNVIDPP IYARFIRILP WSWYGRITLR SELLGCAEEE
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Promotes adhesion of endothelial cells through interaction with the alpha-v/beta-3 integrin receptor. Inhibits formation of vascular-like structures. May be involved in regulation of vascular morphogenesis of remodeling in embryonic development.
Gene References into Functions
  1. these results indicate a protective role for Del-1 in postoperative peritoneal adhesion formation. PMID: 29684347
  2. these data reveal an unrecognised function of endogenous Del-1 as a local inhibitor of ischaemia-induced angiogenesis by restraining LFA-1-dependent homing of pro-angiogenic haematopoietic cells to ischaemic tissues PMID: 28447099
  3. Del-1-induced HSC proliferation and myeloid lineage commitment were mediated by beta3 integrin on hematopoietic progenitors. PMID: 28846069
  4. Del-1 intercepted oxLDL before its receptor binding to reduce atherogenesis PMID: 27784857
  5. EDIL3-mediated activation of TGF-beta and ERK signaling could provide therapeutic implications for HCC. PMID: 25980764
  6. Study identified Del-1 as an endogenous homeostatic factor of the CNS protecting from neuroinflammation in the course of CNS autoimmunity PMID: 25385367
  7. Del-1 controls NF-kappaB-activated MIF production in macrophages. PMID: 24504014
  8. The reciprocal regulation between Del-1 and inflammation may contribute to optimally balance the protective and the potentially harmful effects of inflammatory cell recruitment. PMID: 24416060
  9. Bleomycin-induced pulmonary fibrosis increased collagen deposition and TGF-beta production in the lung of Del-1(-/-) mice. PMID: 24525119
  10. Decreased Del-1 expression is associated with salivary gland inflammation in Sjogren syndrome. PMID: 24060278
  11. Observed increased leukocyte accumulation, inflammation, and higher apoptosis in the adrenal glands of Del-1-deficient mice. Del-1 deficiency was also associated with reduced corticosterone and ACTH levels 24 hours after lipopolysaccharide administration. PMID: 23364949
  12. Del-1 suppressed LFA-1-dependent recruitment of neutrophils and IL-17-triggered inflammatory pathology and may thus be a promising therapeutic agent for inflammatory diseases. PMID: 22447028
  13. Del-1 plays a physiological role in the clearance of phosphatidylserine-expressing microparticles by endothelium. PMID: 22388320
  14. The apoptosis-inducing activity of Del1 could be mapped to the third EGF motif, which fitted the consensus sequence CX(D/N)XXXX(F/Y)XCXC, wherein the aspartic acid residue (D) could be beta-hydroxylated. PMID: 20171188
  15. mediates vascular smooth muscle cells adhesion, migration, and proliferation through interaction with integrin alpha(v)beta(3) PMID: 11959660
  16. acts as an angiogenic factor in the context of solid tumor formation; the increase in vascularization accelerates tumor growth through decreased apoptosis PMID: 12074641
  17. Del-1 is expressed in ischemic tissue. Del-1 stimulates angiogenesis, an effect that is dependent on the RGD motif and a second signaling sequence in the discoidin-I-like domain. PMID: 14981004
  18. Demonstration of Del-1 and milk fat globule-EGF-factor 8 (MFG-E8) binding to phosphatidylserine exposed on apoptotic cells suggests the existence of macrophage subsets that use Del-1 or MFG-E8 differently to engulf apoptotic cells. PMID: 15004195
  19. Del1 (Edil3) is induced by a number of growth factors/cytokines including VEGF, indicating the possible involvement of Del1 in tumor angiogenesis. PMID: 15964546
  20. Fusion with the deposition domain of Del1 dramatically alters the distribution of exogenous proteins in mice. PMID: 17701220
  21. Constitutive expression of Del1 in skin does not affect skin vascularity or improve wound healing. PMID: 17764695
  22. Del-1 may participate in modulating cerebral angiogenesis, and that gene transfer of Del-1 may provide a novel and potent means for stimulating cerebral angiogenesis. PMID: 18534562
  23. study describes Del-1 as an anti-adhesive factor that interferes with the integrin LFA-1-dependent leukocyte-endothelial adhesion; Del-1 is an endogenous inhibitor of inflammatory cell recruitment PMID: 19008446

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Subcellular Location
Secreted.
Tissue Specificity
Expressed in angioblasts and early endothelial cells. By embryonic day 13.5, also expressed in a restricted group of non-endothelial cells including chondrocytes and retinal neurons.
Database Links
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