Product Details

Purity

>85% (SDS-PAGE)

Target Names

Fbxw7

Uniprot No.

Q8VBV4

Alternative Names

Fbxw7; Fbw7; Fbwd6; Fbxw6F-box/WD repeat-containing protein 7; F-box and WD-40 domain-containing protein 7; F-box protein FBW7; F-box protein Fbxw6; F-box-WD40 repeat protein 6; SEL-10

Species

Mus musculus (Mouse)

Expression Region

1-629

Target Protein Sequence

MRVCVPSSVL VLSCVCWCWG VLLPVPLPNL PFLACLSMST LESVTYLPEK GLYCQRLPSS RTHGGTESLK GKNTENMGFY GTLKMIFYKM KRKLDHGSEV RSFSLGKKPC KVSDYTSTTG LVPCSATPTT FGDLRAANGQ GQQRRRITSV QPPTGLQEWL KMFQSWSGPE KLLALDELID SCEPTQVKHM MQVIEPQFQR DFISLLPKEL ALYVLSFLEP KDLLQAAQTC RYWRILAEDN LLWREKCKEE GIDEPLHIKR RKIIKPGFIH SPWKSAYIRQ HRIDTNWRRG ELKSPKVLKG HDDHVITCLQ FCGNRIVSGS DDNTLKVWSA VTGKCLRTLV GHTGGVWSSQ MRDNIIISGS TDRTLKVWNA ETGECIHTLY GHTSTVRCMH LHEKRVVSGS RDATLRVWDI ETGQCLHVLM GHVAAVRCVQ YDGRRVVSGA YDFMVKVWDP ETETCLHTLQ GHTNRVYSLQ FDGIHVVSGS LDTSIRVWDV ETGNCIHTLT GHQSLTSGME LKDNILVSGN ADSTVKIWDI KTGQCLQTLQ GPSKHQSAVT CLQFNKNFVI TSSDDGTVKL WDLKTGEFIR NLVTLESGGS GGVVWRIRAS NTKLVCAVGS RNGTEETKLL VLDFDVDMK

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Related Products

Target Background

Function

Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes and binds phosphorylated sites/phosphodegrons within target proteins and thereafter bring them to the SCF complex for ubiquitination. Mediates ubiquitination and subsequent degradation of CCNE1 and MYC. Identified substrates include cyclin-E (CCNE1 or CCNE2), DISC1, JUN, MYC, NOTCH1 released notch intracellular domain (NICD), NOTCH2, MCL1 and probably PSEN1. Acts as a negative regulator of JNK signaling by binding to phosphorylated JUN and promoting its ubiquitination and subsequent degradation. SCF(FBXW7) complex mediates the ubiquitination and subsequent degradation of NFE2L1. Involved in bone homeostasis and negative regulation of osteoclast differentiation. Regulates the amplitude of the cyclic expression of hepatic core clock genes and genes involved in lipid and glucose metabolism via ubiquitination and proteasomal degradation of their transcriptional repressor NR1D1; CDK1-dependent phosphorylation of NR1D1 is necessary for SCF(FBXW7)-mediated ubiquitination. Also able to promote 'Lys-63'-linked ubiquitination in response to DNA damage. The SCF(FBXW7) complex facilitates double-strand break repair following phosphorylation by ATM: phosphorylation promotes localization to sites of double-strand breaks and 'Lys-63'-linked ubiquitination of phosphorylated XRCC4, enhancing DNA non-homologous end joining.

Gene References into Functions

Loss of Fbxw7 in the presence of BrafV600E mutation is consequential and sufficient to drive melanoma development. PMID: 28581198
A novel mouse line carrying a conditional knockin allele of a cancer-specific FBXW7 mutation was established for carcinogenesis study. PMID: 29386660
results thus suggest that Fbxw7 controls the transcription of MyRF target genes in various tissues through regulation of MyRF protein stability in a manner dependent on MyRF phosphorylation by GSK-3. PMID: 29472293
FBXW7 is critical for RIG-I stabilization during antiviral responses. PMID: 28287082
FBXW7 is markedly downregulated in the liver of obese mice. Mechanistically, FBXW7 directly binds to hepatokine fetuin-A to induce its ubiquitination and subsequent proteasomal degradation, comprising an important mechanism maintaining glucose homeostasis. PMID: 29475832
the regulatory crosstalk between KLF5, miR-29a, and Fbw7/CDC4 cooperatively promotes atherosclerotic development PMID: 29074464
found that Fbw7 loss caused activation of NF-kappaB signaling. Thus, FBW7 plays a protective role in acute intestinal inflammation by modulating the inflammatory response of NF-kappaB pathway. PMID: 29550488
EglN2 might act as an FBW7 ubiquitin ligase substrate contributing to the progression of triple negative breast cancer. PMID: 28036276
These findings highlight the molecular basis of Hajdu-Cheney syndrome (HCS) pathogenesis and provide clinical insights into potential targeted therapeutic strategies for skeletal disorders associated with the aberrant FBW7/NOTCH2 pathway as observed in patients with HCS. PMID: 29149593
The findings reveal a PLK1-Fbw7-Myc signaling circuit that underlies tumorigenesis and validate PLK1 inhibitors, alone or with Bcl2 antagonists, as potential effective therapeutics for MYC-overexpressing cancers. PMID: 27773673
Fbxw7 suppresses KrasG12D-induced pancreatic tumorigenesis via a Yap-dependent mechanism. PMID: 27764699
Myoblast differentiation potential and muscle regeneration can be regulated by Fbxw7beta. PMID: 27594513
Study identifies a REV-ERBalpha post-translational regulatory circuit in which cyclin-dependent kinase 1 (CDK1) phosphorylation of REV-ERBalpha is recognized by the F-box protein, FBXW7alpha, to direct REV-ERBalpha degradation via the proteasome. Disruption of this CDK1-FBXW7-mediated REV-ERBalpha degradation pathway in mouse liver alters circadian rhythmicity, in particular amplitude, and whole-body lipid/glucose home... PMID: 27238018
Gene expression profiling reveals transcriptional regulation by Fbxw7/mTOR pathway in radiation-induced mouse thymic lymphomas. PMID: 26575021
Prion infection induced the expression of FBXW7 in brain. PMID: 25579381
FBXW7 facilitates nonhomologous end-joining via K63-linked polyubiquitylation of XRCC4 in tumor cells. PMID: 26774286
data demonstrate that Fbw7alpha negatively regulates osteogenesis by targeting Runx2 for ubiquitin-mediated degradation in a GSK3beta-dependent manner PMID: 26542806
In mice, an unusually direct antagonism between an E3 ligase and a deubiquitinase, Fbw7 and Usp28, modulate intestinal homeostasis and cancer. PMID: 25716680
These data show that cell cycle-dependent mechanisms can control ciliary length through a CDK5-FBW7-NDE1 pathway. PMID: 26206584
Dual regulation of Fbw7 activity by Usp28 is a safeguard mechanism for maintaining physiological levels of proto-oncogenic Fbw7 substrates, which is equivalently disrupted by loss or overexpression of Usp28. PMID: 25437563
Ubiquitin-dependent degradation of GATA 2 is promoted by Fbw7, is cyclin B-CDK1-mediated Thr176 phosphorylation-dependent, and influences hematopoietic cell differentiation. PMID: 25670854
Fbw7 is a master regulator of cell fate decisions in the pancreas PMID: 25105579
FBXW7 modulates cellular stress response and metastatic potential through HSF1 post-translational modification. PMID: 25720964
these results suggest that FBXW7 antagonizes cancer development in not only a cell-autonomous manner, but also a non-cell-autonomous manner PMID: 25555218
demonstrate the importance of Fbw7-dependent cyclin E control to the hematopoietic system and highlight chromosome instability as a characteristic feature of dysfunction and malignancy induced by deregulated cyclin E PMID: 24958101
p50 upregulated c-Myc protein expression mainly through inhibiting its degradation. p50 exhibited this novel property by suppression of FBW7 expression. PMID: 24457827
Data propose that control of GATA3 levels by Fbw7 contributes to the fine-tuning of T-cell development. PMID: 24820417
FBXW7 regulates spermatogonial stem cell self-renewal in a negative manner by degradation of MYC PMID: 24879440
Heterozygous Fbxw7 propellor tip (R482Q) mutations promote intestinal tumors in mice. Heterozygous null Fbxw7 mutations also promote tumours, but the effect is weaker than R482Q. Findings explain the FBXW7 mutation spectrum found in human cancers. PMID: 23676439
Fbw7 together with GSK3beta negatively regulates G-CSFR expression and its downstream signaling. PMID: 23820376
the downstream Notch signalling effector HES5 directly represses transcription of the E3 ligase Fbw7beta. PMID: 23776410
Loss of parkin function through biallelic mutation of PARK2 may lead to death of dopaminergic neurons through unregulated SCF(Fbw7beta)-mediated ubiquitylation-dependent proteolysis of Mcl-1. PMID: 23858059
inhibition of mTOR signaling pathway suppresses the contribution of Fbxw7 loss toward tumor development. PMID: 23454868
Fbxw7 controls proliferation and differentiation of keratinocytes, and exerts both inhibitory and stimulatory actions in skin carcinogenesis by counteracting the proliferation-promoting effect of c-Myc and tumor-suppressive effect of Notch. PMID: 22665065
findings show that Fbxw7 plays a pivotal role in maintenance of quiescence in nondividing leukemia-initiating cells of chronic myeloid leukemia by reducing the level of c-Myc PMID: 23518349
We demonstrate here the essential function of the Fbw7 E3 ligase for the initiation and the progression of chronic myelogenous leukemia. PMID: 23518350
a number of miR-25 candidate gene targets PMID: 22912667
SCF(Fbw7) modulates the NFkB signaling pathway by targeting NFkB2 for ubiquitination and destruction. PMID: 22708077
Fbxw7 is a potent positive regulator of angiogenesis that limits the activity of Notch in the endothelium of the growing vasculature. PMID: 22848434
Depletion of Fbxw7 resulted in promotion of induced pluripotent stem cell generation. PMID: 22897623
Fbxw7 was decreased in NAFLD and negatively correlated with SREBP-1, indicating that the Fbxw7-SREBP-1 axis may play a key pathological role in the development of NAFLD. PMID: 22710480
identify Fbxw7 as a p53-dependent tumor susceptibility gene. PMID: 22348067
The F-box protein Fbw7 is required for cerebellar development. PMID: 21827743
Fbxw7 as a key regulator of the maintenance and differentiation of neural stem cells in the brain. PMID: 21349854
Loss of Fbxw7 is associated with oxidative stress. PMID: 21205095
FBXW7 influences murine intestinal homeostasis and cancer, targeting Notch, Jun, and DEK for degradation. PMID: 21282377
the E3 ubiquitin ligase SCF(FBW7) governs cellular apoptosis by targeting MCL1, a pro-survival BCL2 family member, for ubiquitylation and destruction in a manner that depends on phosphorylation by glycogen synthase kinase 3 PMID: 21368833
Hepatic ablation of Fbxw7 resulted in hepatomegaly and steatohepatitis, with massive deposition of triglyceride, a phenotype similar to that observed in humans with nonalcoholic steatohepatitis. PMID: 21123947
C/EBPdelta directly inhibits expression of the tumour suppressor F-box and WD repeat-domain containing 7 gene (FBXW7, FBW7, AGO, Cdc4), encoding an F-box protein that promotes degradation of the mammalian target of rapamycin (mTOR). PMID: 21076392
miR-223 expression is responsive to acute alterations in cyclin E regulation by the Fbw7 pathway. PMID: 20826802

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Subcellular Location

Nucleus, nucleoplasm. Chromosome.

Tissue Specificity

Widely expressed with highest levels in brain, heart and testis.

Database Links

KEGG: mmu:50754

STRING: 10090.ENSMUSP00000103305

UniGene: Mm.196475

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Recombinant Mouse F-box/WD repeat-containing protein 7 (Fbxw7)

Product Details

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