Recombinant Mouse Guanylate cyclase soluble subunit alpha-3 (Gucy1a3)

Code CSB-YP861695MO
MSDS
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Source Yeast
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Code CSB-EP861695MO
MSDS
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Source E.coli
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Code CSB-EP861695MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP861695MO
MSDS
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Source Baculovirus
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Code CSB-MP861695MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Gucy1a1
Uniprot No.
Alternative Names
Gucy1a1; Gucy1a3Guanylate cyclase soluble subunit alpha-1; GCS-alpha-1; EC 4.6.1.2; Guanylate cyclase soluble subunit alpha-3; GCS-alpha-3; Soluble guanylate cyclase large subunit
Species
Mus musculus (Mouse)
Expression Region
1-691
Target Protein Sequence
MFCRKFKDLK ITGECPFSLL APGQVPKEPT EEVAGGSEGC QATLPICQYF PEKNAEGSLP QRKTSRNRVY LHTLAESICK LIFPECERLN LALQRTLAKH KIEENRKSSE KEDLEKIIAE EAIAAGAPVE ALKDSLGEEL FKICYEEDEH ILGVVGGTLK DFLNSFSTLL KQSSHCQEAE RRGRLEDASI LCLDKDQDFL NVYYFFPKRT TALLLPGIIK AAARILYESH VEVSLMPPCF RSDCTEFVNQ PYLLYSVHVK STKPSLSPGK PQSSLVIPAS LFCKTFPFHF MLDRDLAILQ LGNGIRRLVN KRDFQGKPNF EEFFEILTPK INQTFSGIMT MLNMQFVIRV RRWDNSVKKS SRVMDLKGQM IYIVESSAIL FLGSPCVDRL EDFTGRGLYL SDIPIHNALR DVVLIGEQAR AQDGLKKRLG KLKATLEHAH QALEEEKKRT VDLLCSIFPS EVAQQLWQGQ IVQAKKFSEV TMLFSDIVGF TAICSQCSPL QVITMLNALY TRFDQQCGEL DVYKVETIGD AYCVAGGLHR ESDTHAVQIA LMALKMMELS NEVMSPHGEP IKMRIGLHSG SVFAGVVGVK MPRYCLFGNN VTLANKFESC SVPRKINVSP TTYRLLKDCP GFVFTPRSRE ELPPNFPSDI PGICHFLDAY HHQGPNSKPW FQDKDVEDGN ANFLGKASGI D
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Gene References into Functions
  1. Exogenous or paracrine sources of NO promote the specification towards the myocyte lineage and expression of cardiac sarcomeric proteins of adult cardiac progenitor cells. This is contingent upon the expression and activity of the alpha1 subunit of guanylyl cyclase in CPC that is necessary for NO-mediated inhibition of the canonical Wnt/beta-catenin pathway. PMID: 27520736
  2. These data demonstrate that a reduction in cardiac sGC activity worsens doxorubicin-induced cardiotoxicity in mice and identify sGC as a potential therapeutic target. PMID: 27505125
  3. We suggest that Gucy1a3 activity promotes the phenotypic switching of smooth muscle cells from a contractile to a synthetic state, fostering the formation of atherosclerosis. PMID: 27315776
  4. The authors elucidated this dependency by showing that guanylate cyclase-1 is a novel rhodopsin-binding protein. PMID: 26590321
  5. Cellular markers revealed that vascular SMC differentiation decreased, whereas myofibroblast activation increased in the hyperoxic sGC-alpha1 KO pup lung. PMID: 23934926
  6. Soluble guanylate cyclase alpha1-deficient mice: a novel murine model for primary open angle glaucoma. PMID: 23527308
  7. Male mice deficient in a NO receptor component, the alpha1 subunit of soluble guanylate cyclase (sGCalpha1), are prone to hypertension in some, but not all, mouse strains, suggesting that additional genetic factors contribute to the onset of hypertension PMID: 22565307
  8. results show that the loss of sGCalpha1 has a positive inotropic and lusitropic effect on basal cardiac function, indicating an important role for sGCalpha1 in regulating basal myocardial contractility PMID: 21394564
  9. RD3 mediates the export of GC1 from the endoplasmic reticulum to endosomal vesicles, and that the C terminus of GC1 is required for RD3 binding PMID: 21078983
  10. Requirement of both alpha-2 and alpha-1 guanylate cyclases for signaling in long-term potentiation (LTP) indicates the existence of two distinct nitric oxide/cGMP-mediated pathways, which have to work in concert for expression of LTP. PMID: 17251421
  11. sGCalpha1 is essential for NO-mediated pulmonary vasodilation and limits chronic hypoxia-induced pulmonary vascular remodeling PMID: 17679618
  12. guanylate cyclase has a role in pulmonary arterial hypertension PMID: 18550612
  13. NFATc3 and HuR are important regulators of sGC-alpha1 expression in pulmonary vascular smooth muscle cells during chronic hypoxia-induced pulmonary hypertension. PMID: 19592461

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Subcellular Location
Cytoplasm.
Protein Families
Adenylyl cyclase class-4/guanylyl cyclase family
Database Links
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