Product Details

Purity

>85% (SDS-PAGE)

Target Names

Ikbke

Uniprot No.

Q9R0T8

Alternative Names

Ikbke; Ikke; IkkiInhibitor of nuclear factor kappa-B kinase subunit epsilon; I-kappa-B kinase epsilon; IKK-E; IKK-epsilon; IkBKE; EC 2.7.11.10; Inducible I kappa-B kinase; IKK-i

Species

Mus musculus (Mouse)

Expression Region

1-717

Target Protein Sequence

MQSTTNYLWH TDDLLGQGAT ASVYKARNKK SGEVVAVKVF NSASYRRPPE VQVREFEVLR RLNHQNIVKL FAVEETGGSR QKVLIMEYCS SGSLLSVLED PENTFGLSEE EFLVVLRCVV AGMNHLRENG IVHRDIKPGN IMRLVGEEGQ SIYKLSDFGA ARKLDDDEKF VSVYGTEEYL HPDMYERAVL RKPQQKAFGV TVDLWSIGVT LYHAATGSLP FIPFGGPRRN KEIMYRITTE KPAGAISGTQ KQENGPLEWS YSLPITCRLS MGLQNQLVPI LANILEVEED KCWGFDQFFA ETSDILQRTV IHVFSLPQAV LHHVYIHAHN TIAIFLEAVY EQTNVTPKHQ EYLFEGHPCV LEPSLSAQHI AHTAASSPLT LFSMSSDTPK GLAFRDPALD VPKFVPKVDL QADYSTAKGV LGAGYQALWL ARVLLDGQAL MLRGLHWVLE VLQDTCQQTL EVTRTALLYL SSSLGTERFS SGAGMPDVQE RKEATELRTR LQTLSEILSK CSHNVTETQR SLSCLGEELL KNRDQIHEDN KSIQKIQCCL DKMHFIYKQF KKSRMRPGLS YNEEQIHKLD KVNFSHLAKR LLQVFQEECV QTYQVSLVTH GKRMRQVQRA QNHLHLIGHS VATCNSEARG AQESLNKIFD QLLLDRASEQ GAEVSPQPMA PHPGPDPKDL VFHMQELCND MKLLAFDLQD NNRLIERLHR VPSAPDV

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Serine/threonine kinase that plays an essential role in regulating inflammatory responses to viral infection, through the activation of the type I IFN, NF-kappa-B and STAT signaling. Also involved in TNFA and inflammatory cytokines, like Interleukin-1, signaling. Following activation of viral RNA sensors, such as RIG-I-like receptors, associates with DDX3X and phosphorylates interferon regulatory factors (IRFs), IRF3 and IRF7, as well as DDX3X. This activity allows subsequent homodimerization and nuclear translocation of the IRF3 leading to transcriptional activation of pro-inflammatory and antiviral genes including IFNB. In order to establish such an antiviral state, IKBKE forms several different complexes whose composition depends on the type of cell and cellular stimuli. Thus, several scaffolding molecules including IPS1/MAVS, TANK, AZI2/NAP1 or TBKBP1/SINTBAD can be recruited to the IKBKE-containing-complexes. Activated by polyubiquitination in response to TNFA and interleukin-1, regulates the NF-kappa-B signaling pathway through, at least, the phosphorylation of CYLD. Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. In addition, is also required for the induction of a subset of ISGs which displays antiviral activity, may be through the phosphorylation of STAT1 at 'Ser-708'. Phosphorylation of STAT1 at 'Ser-708' seems also to promote the assembly and DNA binding of ISGF3 (STAT1:STAT2:IRF9) complexes compared to GAF (STAT1:STAT1) complexes, in this way regulating the balance between type I and type II IFN responses. Protects cells against DNA damage-induced cell death. Also plays an important role in energy balance regulation by sustaining a state of chronic, low-grade inflammation in obesity, wich leads to a negative impact on insulin sensitivity. Phosphorylates AKT1.

Gene References into Functions

IKKepsilon promotes NFATc1 phosphorylation and inhibits T cell responses, identifying IKKepsilon as a crucial negative regulator of T cell activation and a potential target for immunotherapy. PMID: 27346349
Virus-activated kinase IKKepsilon phosphorylated YAP at Ser403 and thereby triggered degradation of YAP in lysosomes and, consequently, relief of YAP-mediated inhibition of the cellular antiviral response. PMID: 28481329
REGgamma-mediated control of IkappaBvarepsilon contributes to NFkappaB activation and promotes bowel inflammation and associated tumour formation in response to chronic injury PMID: 26899380
The results highlight the importance of the C-terminal region in the functional activity of IKKepsilon in innate immune response PMID: 24722368
tic ablation of IKBKE resulted in enhanced and prolonged priming of the NLRP3 inflammasome in cultured macrophages, in hypertrophic adipose tissue, and in livers of hypercholesterolemic mice. PMID: 25540417
novel insight into IKKepsilon, which is involved in adipose tissue inflammation during the development of obesity PMID: 25130737
miR-339-5p inhibits expression of the pro-inflammatory factors in alcohol-induced brain inflammation through the NF-kappaB pathway by abolishing IKK-beta and IKK-epsilon activity. PMID: 25172655
IKKepsilon deficiency inhibits inflammation and induces cardiac protection in high-fat diet-induced obesity in mice. PMID: 24789209
IKKepsilon is expressed in neurons and is upregulated in the hypothalamus of obese mice, contributing to insulin and leptin resistance. PMID: 24812431
Data indicate the role for immune-response functions of IkappaBepsilon in B cells; it regulates proliferative capacity via at least two mechanisms involving cRel- and RelA-containing NF-kappaB dimers. PMID: 24591377
IKKepsilon knockout prevents high fat diet induced arterial atherosclerosis and NF-kappaB signaling in mice. PMID: 23741427
IKBKE regulates FOXO3a primarily through phosphorylation of SerS644 PMID: 23691078
IKKvarepsilon might play an important role in ox-LDL-induced injury of endothelial cells. PMID: 23261326
IKKi deficiency exacerbates cardiac hypertrophy by regulating the AKT and NF-kappaB signaling pathway PMID: 23349709
Upon IL-1 stimulation, IKKi was activated to mediate GSK3alpha phosphorylation at S21. PMID: 23142783
Endotoxin uses a distinct pathway to initiate low-grade inflammation in macrophages, requiring interaction with IRAK-1 upstream of inducible kappa B kinase epsilon (inducible kappa B kinase epsilon) to contribute to the induction of C/EBPdelta and proinflammatory mediators. PMID: 21357541
IkappaB kinase epsilon (IKK(epsilon)) regulates the balance between type I and type II interferon responses. PMID: 22171011
we concluded that IKKepsilon modulates inflammatory nociceptive sensitivity PMID: 21813779
these data indicate that IKKepsilon does not have general pro-inflammatory properties in liver and adipose tissue, and suggest that reduced adiposity is the primary mechanism for improved insulin sensitivity in IKKepsilon(-/-) mice on chow diet. PMID: 21371440
these data suggest alternative splicing as a novel regulatory mechanism suitable to shift the balance between different functions of IKKepsilon. PMID: 21182093
IKKi/IKKepsilon plays a key role in integrating signals induced by pro-inflammatory stimuli. PMID: 12736252
In stimulated murine fibroblast-like synoviocyte cultures, induction of matrix metalloproteinases MMP3 and MMP13 is significantly greater in the IKK epsilon-positive cells compared with IKK epsilon-deficient cultures. PMID: 15879144
Ikbke is essential and for the regulation of IFN responses in innate immune cells except plasmacytoid DCs. PMID: 17056502
Antiviral gene expression was also reduced in FLS from IKKepsilon(-/-) mice compared with IKKepsilon(+/+) mice. PMID: 17328045
findings demonstrate that IKKepsilon is activated by IFNbeta & that IKKepsilon directly phosphorylates STAT1, a component of ISGF3; we conclude that IKKepsilon plays a critical role in IFN-inducible antiviral transcriptional response PMID: 17332413
Gene expression analyses indicate that IKKepsilon knockout reduces expression of inflammatory cytokines, and changes expression of certain regulatory proteins and enzymes involved in glucose and lipid metabolism PMID: 19737522

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Subcellular Location

Cytoplasm. Nucleus. Nucleus, PML body.

Protein Families

Protein kinase superfamily, Ser/Thr protein kinase family, I-kappa-B kinase subfamily

Tissue Specificity

Expressed in bone marrow-derived macrophages and at low levels in liver and white adipose tissue (at protein level). Detected in muscle and lung.

Database Links

KEGG: mmu:56489

STRING: 10090.ENSMUSP00000054126

UniGene: Mm.386783

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Recombinant Mouse Inhibitor of nuclear factor kappa-B kinase subunit epsilon (Ikbke)

Product Details

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