Recombinant Mouse Insulin-like growth factor-binding protein 3 (Igfbp3)

Code CSB-YP011097MO
MSDS
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Source Yeast
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Code CSB-EP011097MO
MSDS
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Source E.coli
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Code CSB-EP011097MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP011097MO
MSDS
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Source Baculovirus
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Code CSB-MP011097MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Igfbp3
Uniprot No.
Alternative Names
Igfbp3; Igfbp-3; Insulin-like growth factor-binding protein 3; IBP-3; IGF-binding protein 3; IGFBP-3
Species
Mus musculus (Mouse)
Expression Region
28-292
Target Protein Sequence
GAG AVGAGPVVRC EPCDARALSQ CAPPPTAPAC TELVREPGCG CCLTCALREG DACGVYTERC GTGLRCQPRP AEQYPLRALL NGRGFCANAS AAGSLSTYLP SQPAPGNISE SEEEHNAGSV ESQVVPSTHR VTDSKFHPLH AKMDVIKKGH ARDSQRYKVD YESQSTDTQN FSSESKRETE YGPCRREMED TLNHLKFLNV LSPRGVHIPN CDKKGFYKKK QCRPSKGRKR GFCWCVDKYG QPLPGYDTKG KDDVHCLSVQ SQ
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R. Promotes testicular germ cell apoptosis.
Gene References into Functions
  1. Igfbp3-null mouse model was generated in combination with Kras(G12D) to compare the tumor burden. Then, IGF-dependent signaling was assessed after expressing wild-type or a mutant IGFBP3 without IGF binding capacity in non-small cell lung cancer (NSCLC) cells PMID: 28330997
  2. endogenous (circulating and/or stromal) IGFBP-3 is stimulatory to adipose tissue expansion and enhances mammary tumor growth in immune-competent mice, potentially by suppressing T-cell infiltration into tumors. PMID: 27448965
  3. High Igfbp3 expression is associated with skin squamous cell carcinoma. PMID: 28807942
  4. The study shows that the anti-tumoral effect of IGFBP-3 is due to inhibition of the Wnt pathway and depends upon the presence of CD44, a receptor protein known to modulate Wnt signaling. PMID: 27377812
  5. IGFBP-3 influences severity of DSS-induced colitis. PMID: 27253188
  6. our studies found that the deletion of IGFBP3 results in behavioral impairments that are associated with abnormal synaptic function and monoaminergic neurotransmission, which helps to characterize the critical role of IGFBP3 in the brain PMID: 28088287
  7. IGFBP-3 is an aggravating factor during hepatic ischemia-repefusion injury. PMID: 26073647
  8. An increase in IGFBP-3 post-trauma may play an important role in limiting trauma-induced inflammatory and apoptotic pathways leading to retinal damage. PMID: 25082650
  9. Even though knockout of IGFBP-3 is associated with only a subtle phenotype under control conditions, our results reveal that loss of this gene has measurable effects on breast carcinogenesis and breast cancer metastasis. PMID: 25614235
  10. Igfbp3 is a marker for culture-activated hepatic stellate cells and plays a role in HSC migration. PMID: 24358328
  11. IGFBP-3 has novel protective effects on retinal and systemic vasculature and may be a therapeutic candidate for ocular complications such as diabetic retinopathy. PMID: 22792172
  12. IGFBP-3 activates inhibitory Smad signaling in 3T3-L1 cells and that endogenous IGFBP-3 modulates their adipogenic differentiation by regulating cell sensitivity towards the inhibitory effect of TGFbeta. PMID: 22910030
  13. Wnt signaling exerts an antiproliferative effect on adult cardiac progenitor cells through IGFBP3. PMID: 22034491
  14. IGFBP-3 positively and negatively impacts on IGF1-induced lung cancer development. PMID: 21447628
  15. Bone morphogenetic protein-1 processes insulin-like growth factor-binding protein 3. PMID: 21697095
  16. Mice genetically deficient in IGFBP-3 have weaker growth of primary prostate tumors but higher incidence of metastases. PMID: 21697285
  17. The IGFBP-3/IGFBP-3R system therefore plays a pivotal role in the pathogenesis of asthma and can serve as a newly identified potential therapeutic target for this debilitating disease. PMID: 21383009
  18. IGFBP-3 has a role in enhanced pericyte ensheathment, reduced microglial activation, increased microglial apoptosis, and neuronal protection after ischemic retinal injury PMID: 21435441
  19. The authors showed that expression of insulin-like growth factor binding protein 3 mRNA increases not only in Borna disease virus P-expressing cultured cells but also in astrocytes from the cerebella of P transgenic mice. PMID: 21325425
  20. Association between Igfbp3 and risk of prostatic cancer are examined. PMID: 20473871
  21. This study provides evidence that genetic deletion of IGFBP-3 modulates hepatic carbohydrate and lipid metabolism. PMID: 20926583
  22. Data indicate that impairment of IGF-1 complex formation with insulin-like growth factor binding protein, acid labile subunit in bone marrow alters cell fate, leading to increased adipogenesis. PMID: 20007694
  23. IGFBP3 and Humanin play key roles in the coordinated regulation of testicular germ cell homeostasis. PMID: 19952275
  24. ta indicate that Zik1 and Gja9 demonstrated cancer-specific aberrant DNA methylation, whereas, Cdkn2a/p16, Igfbp3, Mgmt, Id4, and Cxcr4 were methylated in both the AOM tumors and normal colon mucosa. PMID: 19777566
  25. response to nutritional stsatus chanes in PPARalpha knockout mice PMID: 11735249
  26. overexpression of hIGFBP-3 results in fasting hyperglycemia, impaired glucose tolerance, and insulin resistance. PMID: 12376320
  27. IGFBP-3 increases osteoclast number and bone resorption, impairs osteoblast proliferation, and has a significant negative effect on bone formation PMID: 14584894
  28. N- and C-terminal residues of IGFBP-3 have roles in regulating IGF complex formation and receptor activation PMID: 15485880
  29. the growth inhibitory effect of mutant IGFBP-3 may be compensated for by other mechanisms PMID: 15550509
  30. IGFBP-3 induces apoptosis through an insulin-like growth factor-independent nucleomitochondrial translocation of RXRalpha/Nur77 PMID: 15731112
  31. Overexpression of Hbegf in transgenic mice downregulates Igfbp3. PMID: 16019424
  32. Igfbp3 modulates cell proliferation in the hair follicle. PMID: 16185287
  33. IGFBP-3 is upregulated by apigenin in a pathway leading to cancer growth inhibition and apoptosis PMID: 16230333
  34. the IGF-independent effects of IGFBP-3 may be important in inhibiting tumor progression PMID: 16469805
  35. IGFBP-3 is a growth inhibitor molecule (news) PMID: 16617154
  36. provide direct evidence for combinatorial effects of IGFBP-3, -4, and -5 in both metabolism and at least some soft tissues PMID: 16675541
  37. IGFBP-3 has direct, IGF-independent inhibitory effects on angiogenesis providing an additional mechanism by which it exerts its tumor suppressive effects PMID: 16983336
  38. IGFBP3 is a downstream gene regulated by MeCP2 and MeCP2 may contribute directly to the transcriptional expression of IGFBP3 in the brain. PMID: 17278996
  39. In summary, the absence of the IGFBP-5 gene did not block the ability of IGF-I to stimulate intestinal growth, possibly because greater jejunal expression of IGFBP-3 compensates for the absence of IGFBP-5. PMID: 17332154
  40. IGFBP-3 induces angiogenesis through IGF-I- and SphK1-dependent mechanisms PMID: 17388800
  41. Igfbp3 regulates hematopoietic stem cell and endothelial precurosr cell function during vascular development. PMID: 17567755
  42. Igfbp3 suppresses retinopathy through suppression of oxygen-induced vessel loss and promotion of vascular regrowth. PMID: 17567756
  43. SRC-3 maintains IGF-I in the circulation through enhancing VDR-regulated IGFBP-3 expression. PMID: 18212051
  44. Data suggest that loss of expression of IGFBPs (IGFBP-3 and IGFBP-4) in tumor cells treated with EGFR tyrosine kinase inhibitors derepresses IGFIR signaling, which in turn mediates resistance to EGFR antagonists. PMID: 18568074
  45. IGF-I, IGFBP-1 and IGFBP-3 are all inversely related to adiposity in Hispanic children. PMID: 18924579
  46. IGFBP-3 inhibits adipocyte differentiation. PMID: 19141684
  47. IGFBP3 is a new regulator of hypocretin cell physiology that may be involved not only in the pathophysiology of narcolepsy, but also in the regulation of sleep in normal individuals, most notably during adolescence PMID: 19158946
  48. IGFBP-3 binds to fibronectin cell binding domain. PMID: 19236847
  49. These studies reveal multisite phosphorylation of IGFBP-3 that both positively and negatively regulate its apoptotic potential. PMID: 19556345
  50. IGFBP-3 mediated chondrocyte apoptosis is regulated by Gli2 and p53 in the progression from benign to malignant cartilage tumors. PMID: 19647223

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Subcellular Location
Secreted.
Database Links
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