Recombinant Mouse Interleukin-1 receptor-associated kinase 1(Irak1)

Code CSB-YP011808MO
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Source Yeast
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Code CSB-EP011808MO
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Source E.coli
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Code CSB-EP011808MO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP011808MO
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Source Baculovirus
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Code CSB-MP011808MO
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Irak1
Uniprot No. Q62406
Alternative Names Irak1; Il1rakInterleukin-1 receptor-associated kinase 1; IRAK; IRAK-1; EC 2.7.11.1; Pelle-like protein kinase; mPLK
Species Mus musculus (Mouse)
Expression Region 1-710
Target Protein Sequence MAGGPGPGEP VVPGAQHFLY EVPPWVMCRF YKVMDALEPA DWCQFAALIV RDQTELRLCE RSEQRTASVL WPWINRNARV ADLVHILTHL QLLRARDIIT AWHPPAPVVP PSTAAPRPSS ISAGSEAGDW SPRKLQSSAS TFLSPAFPGS QTHSESELLQ VPLPVSLGPP LPSSAPSSTK SSPESPVSGL QRAHPSPFCW PFCEISQGTC NFSEELRIGE GGFGCVYRAV MRNTTYAVKR LKEEADLEWT MVKQSFLTEV EQLSRFRHPN IVDFAGYCAE SGLYCLVYGF LPNGSLEDQL HLQTQACSPL SWPQRLDILL GTARAIQFLH QDSPSLIHGD IKSSNVLLDE RLMPKLGDFG LARFSRFAGA KASQSSTVAR TSTVRGTLAY LPEEYIKTGR LAVDTDTFSF GVVILETLAG QRAVRTQGAK TKYLKDLIED EAEEAGVTLK STQPTLWVGV ATDAWAAPIA AQIYKKHLDS RPGPCPPQLG LALAQLACCC MHRRAKKRPP MTQVYKRLEG LQAGPPWELE VAGHGSPSPQ ENSYMSTTGS AQSGDEPWQP LVVTTRAPAQ AAQQLQRSPN QPVESDESVP GLSATLHSWH LTPGSHPSPA SFREASCTQG GTTRESSVRS SPGFQPTTME GSPTGSSSLL SSEPPQIIIN PARQKMVQKL ALYEEGVLDS LQLLSSGFFP GLDLEPEKSQ GPEESDEFQS
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation. Association with MYD88 leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates the interferon regulatory factor 7 (IRF7) to induce its activation and translocation to the nucleus, resulting in transcriptional activation of type I IFN genes, which drive the cell in an antiviral state. When sumoylated, translocates to the nucleus and phosphorylates STAT3.
Gene References into Functions
  1. Findings demonstrate that the pro- and anti-inflammatory responses to gAd in innate immune cells are time-dependent, and mediated by the activation of the IkappaB/NF-kappaB pathway, and IRAK-1 downregulation, respectively. PMID: 30078230
  2. suppression of inflammatory responses and T cells apoptosis were observed with elevated expression of IRAK-M and PDL-1, and interference and neutralization of these 2 molecules led to partly reversed suppression of inflammation. PMID: 29995830
  3. The expression of IL-1 receptor-associated kinase 1 (IRAK1) that regulates NF-kappaB, p38, and the JNK signaling pathways, was also increased by MPQP. PMID: 29804558
  4. role of microRNA-146a in regulating the expression of IRAK1 in cerebral ischemia-reperfusion injury PMID: 29505740
  5. These findings demonstrate that Dectin-1, Irak1, and Rip2 are involved in response to Aspergillus fumigatus infection. Dectin-1 modulates the expression of Irak1 and Rip2. PMID: 29058172
  6. MiR-146a/ MiR-146b could down-regulate the TRAF6 and IRAK1 expression and promote proliferation, migration and angiogenesis ability of endothelial progenitor cells, which could be important for recovery of patients with hyperacute ischemic stroke. PMID: 28811005
  7. miR-146a inhibits inflammatory cytokine production in B cells through directly targeting IRAK1, suggesting a regulatory role of miR-146a in B cell-mediated periodontal inflammation. PMID: 29288795
  8. NLRC3 promoted K48-linked polyubiquitination and degradation of interleukin-1 receptor-associated kinase 1 (IRAK1). PMID: 28731142
  9. these findings identify a transcription-independent, rapid, and nongenomic glucocorticoid suppression of TLR9 ligand-mediated IRAK1 ubiquitination as a novel mechanism for restraining acute inflammatory reactions PMID: 29038250
  10. These results suggest that the IRAK1-binding protein OPTN negatively regulates IL-1beta/LPS-induced NF-kappaB activation by preventing polyubiquitination of TRAF6. PMID: 28882891
  11. Data, including data from studies in knockout mice, suggest that Irak1 is involved in regulating glucose metabolism and insulin sensitivity in skeletal muscle (but not in liver and not in obesity due to high-fat diet); insulin sensitivity is 30% higher in Irak1 knockout mice on chow diet, but Irak1 deletion does not affect development of glucose intolerance due to high-fat diet. PMID: 28572512
  12. Suppression of IRAK1 or IRAK4 Catalytic Activity, but Not Type 1 IFN Signaling, Prevents Lupus Nephritis in Mice Expressing a Ubiquitin Binding-Defective Mutant of ABIN1 PMID: 27807192
  13. Torreya nucifera butanol fraction exhibits anti-inflammatory activities by direct inhibition of macrophage Src/Syk/NF-kappaB and IRAK1/AP-1 signaling. PMID: 27178629
  14. IRAK1 is involved in TLR4-mediated downregulation of ABCA1 expression and lipid accumulation in VSMCs. PMID: 26512959
  15. plays an important role in intestinal inflammation by mediating T cell activation, differentiation, and accumulation in the gut PMID: 26561545
  16. Mangiferin ameliorates colitis by inhibiting IRAK1 phosphorylation in NF-kappaB and MAPK pathways PMID: 24972244
  17. miR-146a ameliorates liver ischemia/reperfusion injury in vivo and hypoxia/reoxygenation injury in vitro by directly suppressing IRAK1 and TRAF6. PMID: 24987958
  18. miR-146a attenuates sepsis-induced cardiac dysfunction by preventing NF-kappaB activation, inflammatory cell infiltration, and inflammatory cytokine production via targeting of IRAK and TRAF6 in both cardiomyocytes and inflammatory monocytic cells. PMID: 26048146
  19. IRAK1 deficiency impacts multiple TLR-dependent pathways and decreases early cytokine responses following polymicrobial sepsis. PMID: 23856940
  20. CD40 signaling induces IRAK1 sumoylation in the presence of TNF receptor-associated factor 2 (TRAF2) and intracellular isoform of osteopontin PMID: 24706909
  21. Mosaicism for IRAK1 expression is accompanied by skewing toward deficient immune cell populations, producing a phenotype that is preconditioned for improved sepsis outcome PMID: 24193737
  22. IRAK-1 bypasses priming and directly links Toll-like receptors to rapid NLRP3 inflammasome activation. PMID: 24379360
  23. Data indicate that IRAK1 is required for LMP1-induced CaMKII activation and p65/RelA serine 536 phosphorylation. PMID: 24248603
  24. These findings define the pathways that lead to rapid NLRP3 activation and identify IRAK1 as a critical mediator of a transcription-independent, inflammasome-dependent early warning response to pathogenic infection. PMID: 24043892
  25. Irak1 accumulation triggers I/R-induced epithelial immune hyper-responsiveness and suggest that the induction of miR-146a offers a promising strategy to prevent I/R tissue injury PMID: 23143987
  26. Data indicate that kalopanaxsaponin B ameliorates lipopolysaccharide (LPS)-induced systemic inflammation by inhibiting IRAK1 kinase. PMID: 23117333
  27. rhinovirus infection delays bacterial clearance in vivo and suppresses non-typeable Hemophilus influenzae-stimulated chemokine responses via degradation of IRAK-1 PMID: 23055935
  28. p16(INK4a) exerts an anti-inflammatory effect through accelerated IRAK1 degradation in macrophages. PMID: 23066149
  29. Endogenous IRAK-M attenuates adverse postinfarction remodeling suppressing leukocyte inflammatory activity, while inhibiting fibroblast-mediated matrix degradation. PMID: 22995519
  30. Low-dose lipopolysaccharide utilizes IRAK-1 and Tollip to induce mitochondrial reactive oxygen species, which subsequently induces activating transcription factor (ATF)2 in chronic low-grade inflammatory disease. PMID: 22706082
  31. TLR2 inhibits IFN-I induction by TLR7/9, may shape immune responses to microbes that express ligands for both TLR2 and TLR7/TLR9, or responses to bacteria/virus coinfection. PMID: 22227568
  32. we have provided first-hand evidence demonstrating the in vivo beneficial effect of IRAK-1 deficiency in LPS-induced oxidative tissue damage and injury PMID: 22364946
  33. Endotoxin uses a distinct pathway to initiate low-grade inflammation in macrophages, requiring interaction with IRAK-1 upstream of IkappaB kinase epsilon to contribute to the induction of C/EBPdelta and proinflammatory mediators. PMID: 21357541
  34. Increased Smad3 linker phosphorylation at Thr-179 and Ser-208 and was dependent on ERK1/2 activity via the TLR4-IRAK1-linked signaling cascade. PMID: 21295571
  35. Although IRAK1 kinase activity is essential for human plasmacytoid dendritic cell activation, it is dispensable in B, T, dendritic, and monocytic cells, which is in contrast with an essential active kinase role in comparable mouse cell types. PMID: 21160042
  36. Leptin increases the expression of IRAK-1 in murine macrophages. IRAK-1 deficient macrophages exhibit significantly lower expression of IL-6 following LPS or LPS plus leptin stimulation. PMID: 20619458
  37. IRAK-1 forms a close complex with PKCepsilon as well as VASP, and participates in phorbol 12-myristate 13-acetate-induced phosphorylation of VASP. PMID: 20044140
  38. Identification and characterization of murine IRAK-M. PMID: 12054681
  39. novel splice site variant activates nuclear factor-kappaB and c-Jun N-terminal kinase PMID: 12418963
  40. TIFA is likely to mediate IRAK-1/TRAF6 interaction upon IL-1 stimulation. PMID: 12566447
  41. IRAK1 function, and by extension Toll/IL-1 signaling, is critical for the induction of experimental autoimmune encephalomyelitis as well as the activation and expansion of autoreactive T cells. PMID: 12626533
  42. lipopolysaccharides (LPS) activate Toll/IL-1 signaling and IL-1 receptor-associated kinase-1 (IRAK1), a critical pathway intermediate in the heart, indicating that the function of this pathway is not limited to immune system tissues. PMID: 12860565
  43. induction of IRAK-M and inhibition of kinase activity of IRAK-1 are crucial to PGN-induced tolerance in macrophages PMID: 14660668
  44. IRAK1 and TRAF6 are essential for LMP1-induced NF-kappaB activation. PMID: 14673102
  45. IRAK-1 plays an important role in the host response to staphylococcal sepsis PMID: 15555532
  46. IRAK-1 is a specific regulator for TLR7- and TLR9-mediated IFN-alpha induction in plasmacytoid dendritic cells. PMID: 15767370
  47. Only LPS, not taxol, caused a dramatic decrease in Irak1 protein levels. PMID: 15829295
  48. IRS-1 is a novel physiological substrate for pelle-like kinase PMID: 15849359
  49. It is therefore concluded that intracellular localization of IRAK-1 depends on its kinase activity and that IRAK-1 may also function as a kinase in the nucleus as shown by a new putative endogenous substrate. PMID: 15896328
  50. Curcumin blocked IRAK thiols in a murine T-cell line stably overexpressing IRAK. PMID: 16046709

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Subcellular Location Cytoplasm. Nucleus. Lipid droplet.
Protein Families Protein kinase superfamily, TKL Ser/Thr protein kinase family, Pelle subfamily
Tissue Specificity Highly expressed in liver, followed by kidney and skeletal muscle.
Database Links

KEGG: mmu:16179

UniGene: Mm.38241

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