Recombinant Mouse Long-chain-fatty-acid--CoA ligase ACSBG2(Acsbg2)

Code CSB-YP652065MO
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Source Yeast
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Code CSB-EP652065MO
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Source E.coli
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Code CSB-EP652065MO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP652065MO
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Source Baculovirus
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Code CSB-MP652065MO
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Acsbg2
Uniprot No. Q2XU92
Alternative Names Acsbg2; BgrLong-chain-fatty-acid--CoA ligase ACSBG2; EC 6.2.1.3; Acyl-CoA synthetase bubblegum family member 2; Arachidonate--CoA ligase ACSBG2; EC 6.2.1.15; Bubblegum-related protein
Species Mus musculus (Mouse)
Expression Region 1-667
Target Protein Sequence MTQEKKAEDL ERGTDATSAV PRLWSTHCDG EVLLRLSKHG PGHETPMTIP ELFQESAERF SAYPALASKN GKKWDTLTFS QYYEMCRKAA KSLIKLGLQR FQCVGILGFN SVEWVVTALG TILAGGLCVG IYATNSAEAC QYVIQQANVS ILIVENDQQL QKILLIPPDK METVKAIVQY KLPLMESMAN LYSWNDFMEL GNDIPNIQLD RVILSQKANQ CAVILYTSGT TGTPKGVLLS HDNITWTAGA MSQEMEINRV SGKQNTIVSY LPLSHIAAQL TDIWIPIKIG ALTFFAQPDA LRGTLVYTLQ EVKPTLFMGV PRIWEKMQDT IKENVARSSR LRKKAFAWAK MLGLKVNTKR MLGKRDIPMN YRMAKALVFA KVRTSLGLDN CHAFFSSASP LSQDVSEFFL SLDIPIGEIY GMSECSGPHT VSNKSVYRVL SCGKVLSGCK NMLYNQNKEG VGEVCMWGRH VFMGYLNKEE ATLEALDENG WLHSGDIGRL DSHDFLYITG RIKEILITAG GENVSPIPIE TLVKEKIPII SHAMLVGDKA KFLCMLLTLK CETDRKSGEP LNKLSVEAKS FCQMLGSQAT TVSDILKSRD QVVYTAIQYG IDIVNQQAMS DSHRIRKWII LEKDFSIQGG ELGPTSKLKR SVITQKYKAQ IDSMYLS
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Catalyzes the conversion of fatty acids such as long chain and very long-chain fatty acids to their active form acyl-CoAs for both synthesis of cellular lipids, and degradation via beta-oxidation. Can activate diverse saturated, monosaturated and polyunsaturated fatty acids. Has increased ability to activate oleic and linoleic acid. May play a role in spermatogenesis.
Gene References into Functions
  1. The limited tissue expression pattern and limited substrate specificity rule out a likely role for this enzyme in X-linked adrenoleukodystrophy pathology. PMID: 16371355
Subcellular Location Cytoplasm. Membrane; Peripheral membrane protein.
Protein Families ATP-dependent AMP-binding enzyme family, Bubblegum subfamily
Tissue Specificity Testis- and brainstem-specific. Expressed in pubertal and adult testis. Enriched in germ cells and Sertoli cells while present at a lower level in Leydig cells. Present in testicular Sertoli cells and large motoneurons in the medulla oblongata and cervica
Database Links

KEGG: mmu:328845

STRING: 10090.ENSMUSP00000042352

UniGene: Mm.179421

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