Recombinant Mouse Lymphocyte-specific helicase (Hells), partial

Code CSB-YP733715MO
MSDS
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Source Yeast
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Code CSB-EP733715MO
MSDS
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Source E.coli
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Code CSB-EP733715MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP733715MO
MSDS
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Source Baculovirus
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Code CSB-MP733715MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Hells
Uniprot No.
Alternative Names
Hells; Lsh; Pasg; Lymphocyte-specific helicase; EC 3.6.4.-; Proliferation-associated SNF2-like protein
Species
Mus musculus (Mouse)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Plays an essential role in normal development and survival. Involved in regulation of the expansion or survival of lymphoid cells. Required for de novo or maintenance DNA methylation. May control silencing of the imprinted CDKN1C gene through DNA methylation. May play a role in formation and organization of heterochromatin, implying a functional role in the regulation of transcription and mitosis.
Gene References into Functions
  1. Lsh assists epigenetic gene repression upon binding to the Oct4 promoter region. PMID: 28621576
  2. These results suggest that Lsh exerts epigenetic regulation at key regulators of neural stem cell fate ensuring adequate neural stem/progenitor cells self-renewal and maintenance during development. PMID: 28442710
  3. fibroblasts derived from chromatin remodeling ATPase LSH (HELLS)-null mouse embryos, which lack DNA methylation from centromeric repeats, transposons and a number of gene promoters, are capable of reestablishing DNA methylation and silencing of misregulated genes upon re-expression of LSH. PMID: 27179028
  4. Data suggest that HELLS controls cytosine methylation in a nuclear compartment that is in part defined by lamin B1 attachment regions. PMID: 25170028
  5. histone H3 lysine 4 methylation modification was assessed in murine embryonic fibroblasts (MEFs) derived from the DNA methylation mutant Lsh(-/-) mice. PMID: 24711395
  6. These results indicate that in the absence of HELLS, proliferation of spermatogonia is reduced and germ cell differentiation arrested at the midpachytene stage, implicating an essential role for HELLS during male meiosis. PMID: 21349825
  7. LSH is essential for developmentally programmed de novo DNA methylation at the promoters of protein coding genes and recruitment of G9a/GLP complex to a subset of genomic loci. PMID: 21149390
  8. Data show that hypomethylation in Lsh-/- cells is associated with efficient transcriptional elongation and splicing. PMID: 20161795
  9. model in which Lsh is recruited by intact heterochromatin structure and then assists in maintaining heterochromatin organization by establishing CpG methylation patterns PMID: 14612388
  10. PASG mutant mice display signs of growth retardation and premature aging, including low birth weight, failure to thrive, graying and loss of hair, reduced skin fat deposition, osteoporosis, kyphosis, cachexia, and premature death. PMID: 15105378
  11. Lsh has a critical and previously unidentified role in epigenetic gene silencing and maintenance of genomic stability during female meiosis. PMID: 17115026
  12. Lsh is part of a physiological feedback loop that reinforces DNA methylation and silencing of polycomb repressive complex targets PMID: 17726103
  13. DNA hypomethylation caused by Lsh depletion is linked to transcriptional upregulation of retroviral elements and oncogenes such as PU.1 which in turn may promote the development of erythroleukemia in mice PMID: 18487951

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Subcellular Location
Nucleus. Note=Closely associated with pericentric heterochromatin.
Protein Families
SNF2/RAD54 helicase family
Tissue Specificity
Highly expressed in thymus, bone marrow, and testis. Not detected in heart, brain, liver, kidney, skeletal muscle, spleen, peripheral blood lymphocytes, small intestine, colon, prostate or ovary.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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