Recombinant Mouse Platelet-derived growth factor D (Pdgfd)

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Code CSB-EP852889MO
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Size US$306
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
Pdgfd
Uniprot No.
Research Area
Others
Alternative Names
Pdgfd; Scdgfb; Platelet-derived growth factor D; PDGF-D; Spinal cord-derived growth factor B; SCDGF-B) [Cleaved into: Platelet-derived growth factor D; latent form; PDGFD latent form); Platelet-derived growth factor D; receptor-binding form; PDGFD receptor-binding form)]
Species
Mus musculus (Mouse)
Source
E.coli
Expression Region
24-370aa
Target Protein Sequence
TPQRASIKALRNANLRRDESNHLTDLYQREENIQVTSNGHVQSPRFPNSYPRNLLLTWWLRSQEKTRIQLSFDHQFGLEEAENDICRYDFVEVEEVSESSTVVRGRWCGHKEIPPRITSRTNQIKITFKSDDYFVAKPGFKIYYSFVEDFQPEAASETNWESVTSSFSGVSYHSPSITDPTLTADALDKTVAEFDTVEDLLKHFNPVSWQDDLENLYLDTPHYRGRSYHDRKSKVDLDRLNDDVKRYSCTPRNHSVNLREELKLTNAVFFPRCLLVQRCGGNCGCGTVNWKSCTCSSGKTVKKYHEVLKFEPGHFKRRGKAKNMALVDIQLDHHERCDCICSSRPPR
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
56.2kDa
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal 6xHis-SUMO-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description

The recombinant Mouse Pdgfd was expressed with the amino acid range of 24-370. The expected molecular weight for the Pdgfd protein is calculated to be 56.2 kDa. This Pdgfd recombinant protein is manufactured in e.coli. The N-terminal 6xHis-SUMO tag was fused into the coding gene segment of Pdgfd, making it easier to detect and purify the Pdgfd recombinant protein in the later stages of expression and purification.

Platelet-derived growth factor D (Pdgfd) primarily functions as a signaling molecule that regulates cell growth and differentiation. It plays a crucial role in processes like wound healing, tissue repair, and angiogenesis. Research areas for Pdgfd applications include investigations into its involvement in tissue regeneration, wound healing mechanisms, and understanding its role in diseases such as cancer, fibrosis, and cardiovascular disorders. Exploring the therapeutic potential of Pdgfd modulation may provide insights into developing interventions for conditions characterized by dysregulated cell growth and tissue repair.

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Target Background

Function
Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Plays an important role in wound healing. Has oncogenic potential and can induce tumor formation. Induces macrophage recruitment, increased interstitial pressure, and blood vessel maturation during angiogenesis. Can initiate events that lead to a mesangial proliferative glomerulonephritis, including influx of monocytes and macrophages and production of extracellular matrix.
Gene References into Functions
  1. Data (including data from studies using transgenic/mutant mice and cells from such mice) suggest that Pdgfd promotes cell proliferation, cell migration, and expression of inflammatory factors in adventitial fibroblasts; in obese mice, inhibition of Pdgfd significantly reduces aortic aneurysm formation (induced by infusion of angiotensin II). PMID: 29794241
  2. upregulated in kidney fibrosis, may mediate renal scarring, and is dispensable for normal kidney development and physiological functions PMID: 26924050
  3. Inactivation of the Pdgfd gene resulted in a mild phenotype in C57BL/6 mice, and the offspring was viable, fertile and generally in good health. PMID: 27032083
  4. PDGF-D intensifies fibrogenesis by interfering with the fibrolytic activity of the TIMP-1/MMP-2/MMP-9 system, and PDGF-D signaling is mediated through both PDGF-alpha and -beta receptors. PMID: 25576870
  5. Radial glia require PDGFD-PDGFRbeta signalling in human but not mouse neocortex PMID: 25391964
  6. PDGF D represents a potentially promising target for prostate carcinoma treatment resistance in the absence of PTEN function, and warrants further laboratory evaluation and clinical study. PMID: 24331662
  7. Data indicate that the proteolytic processing of full-length PDGF-D is required for PDGF-D activation of preosteoclasts, and that beta-PDGFR is present in activated osteoclasts. PMID: 22158043
  8. identified PDGF-DD as an important target gene for antiangiogenic therapy due to its pleiotropic effects on vascular and non-vascular cells. PDGF-DD inhibition may offer new therapeutic options to treat neovascular diseases PMID: 20231273
  9. Failure of laminin alpha4-mediated down-regulation of PDGF activity contributes to the progressive renal lesions in this animal model. PMID: 20035058
  10. These results suggest that PDGF-D induce cellular transformation and promote tumour growth by accelerating the proliferation rate of the tumour cells, and by stimulation of tumour neovascularization. PMID: 12629513
  11. splicing variant results in significant differences in peptide expression and function PMID: 12890490
  12. PDGF-D plays an important role in the pathogenesis of tubulointerstitial injury through binding of PDGF-Rbeta in obstructive ureteral nephropathy PMID: 14514732
  13. PDGF-DD, a novel mediator of smooth muscle cell phenotypic modulation, is upregulated in endothelial cells exposed to atherosclerosis-prone flow patterns. PMID: 19028801

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Subcellular Location
Secreted.
Protein Families
PDGF/VEGF growth factor family
Tissue Specificity
Expressed at high levels in developing heart, lung, kidney and some muscle derivatives. Moderately expressed in liver, brain and testis. In the kidney, localized to glomerular mesangial cells and vascular smooth muscle cells. Up-regulated in areas of rena
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