Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

Cfp

Uniprot No.

P11680

Research Area

Immunology

Alternative Names

Cfp; Pfc; Properdin; Complement factor P

Species

Mus musculus (Mouse)

Source

Yeast

Expression Region

23-464aa

Target Protein Sequence

SDPVLCFTQYEESSGRCKGLLGRDIRVEDCCLNAAYAFQEHDGGLCQACRSPQWSAWSLWGPCSVTCSEGSQLRHRRCVGRGGQCSENVAPGTLEWQLQACEDQPCCPEMGGWSEWGPWGPCSVTCSKGTQIRQRVCDNPAPKCGGHCPGEAQQSQACDTQKTCPTHGAWASWGPWSPCSGSCLGGAQEPKETRSRSCSAPAPSHQPPGKPCSGPAYEHKACSGLPPCPVAGGWGPWSPLSPCSVTCGLGQTLEQRTCDHPAPRHGGPFCAGDATRNQMCNKAVPCPVNGEWEAWGKWSDCSRLRMSINCEGTPGQQSRSRSCGGRKFNGKPCAGKLQDIRHCYNIHNCIMKGSWSQWSTWSLCTPPCSPNATRVRQRLCTPLLPKYPPTVSMVEGQGEKNVTFWGTPRPLCEALQGQKLVVEEKRSCLHVPVCKDPEEKKP
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

49.9kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The first step of making this recombinant Mouse Cfp protein is synthesizing the Cfp gene, the synthesized gene is then cloned into an expression vector which is a cDNA plasmid that includes a promoter sequence and an antibiotic-resistant gene. It also has N-terminal 6xHis tag encoding a fusion tag for downstream protein purification or identification. The antibiotic-resistant gene enables the selection of cells carrying the plasmid in antibiotic-based media and then transfects cells with a DNA vector that contains the template and then culturing the cells so that they transcribe and translate the desired protein. The recombinant Cfp protein is purified by ion-exchange chromatography or affinity purification. And the purity is 90%+ by SDS-PAGE.

Cfp is a protein coding gene that encodes Properdin (Complement factor P). According to some studies, Cfp may have the following features.
Properdin (Complement factor P) is expressed in choroidal neovascularization in 50%+ of AMD patients. Properdin is a pattern recognition molecule that binds to certain microbial surfaces, apoptotic and necrotic cells. Properdin not only stabilizes but also initiates complement activation. Properdin interacts with immune cells and is involved in many immune responses. Properdin is only expressed in the myeloid cell line HL-60, the monocytic cell line U-937 and the monocytic cell line Mono Mac 6.

Target Background

Function

A positive regulator of the alternate pathway of complement. It binds to and stabilizes the C3- and C5-convertase enzyme complexes. Inhibits CFI-CFH mediated degradation of Inhibits CFI-CFH mediated degradation of Complement C3 beta chain (C3b).

Gene References into Functions

The results show that Properdin plays a key role in allergen-induced airway inflammation and represents a potential therapeutic target for human asthma. PMID: 26116506
Authors conclude that properdin controls the strength of immune responses by affecting humoral as well as cellular phenotypes during acute bacterial infection and ensuing inflammation. PMID: 24728387
Properdin deficiency exacerbated renal injury in mice lacking complement factor H. PMID: 23184055
deficiency of properdin in mice with factor H mutation exacerbates C3 glomerulonephritis PMID: 23204401
Murine antigonococcal antiserum required functional properdin to kill C4BP-binding strains, but not C4BP-nonbinding strains. PMID: 22368277
properdin may be added to the list of complement proteins which influence lipid metabolism, energy storage and insulin resistance, and further support the hypothesis of a dual role of complement in adipose tissue PMID: 22387270
At the initial phase of local, zymosan-induced inflammation, properdin-deficient and wild-type mice showed bone erosion, proteoglycan loss and cell infiltration. PMID: 20226532
Properdin localizes to mast cells, has the ability to directly associate with Escherichia coli DH5alpha, and plays a significant role in the outcome of polymicrobial sepsis. PMID: 18292556
properdin deficiency attenuates zymosan-induced shock and exacerbates LPS-induced shock. PMID: 18453618

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Subcellular Location

Secreted.

Database Links

KEGG: mmu:18636

STRING: 10090.ENSMUSP00000001156

UniGene: Mm.3064

Code	CSB-YP005291MO
Abbreviation	Recombinant Mouse Cfp protein
MSDS	MSDS Datasheet
Size	$368
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of CSB-YP005291MO could indicate that this peptide derived from Yeast-expressed Mus musculus (Mouse) Cfp. Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of CSB-YP005291MO could indicate that this peptide derived from Yeast-expressed Mus musculus (Mouse) Cfp.
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Recombinant Mouse Properdin (Cfp)

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